Effects of commercial queen rearing methods on queen fecundity and genome methylation

Yao, Yi; Liu, Yi Bo; Barron, Andrew B.; Zeng, Zhen

doi:10.1007/s13592-020-00817-7

Cited by 9 publications

(21 citation statements)

References 28 publications

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“…(2021); Yi et al (2021) and Yagound et al (2020). We find around 1,500 genes per sample show allele-specific DNA methylation with slightly lower numbers in worker thorax tissue (Fig.…”

Section: Consistency Of Parent-of-origin Dna Methylation In the Honeybeementioning

confidence: 74%

“…We then called allele-specific DNA methylation in independently generated honeybee female data sets (n = 33) spanning various tissues of both workers and queens from Cardoso-Júnior et al . (2021); Yi et al . (2021) and Yagound et al .…”

Section: Resultsmentioning

confidence: 99%

“…(2019, 2020); Cardoso-Júnior et al . (2021); Yi et al . (2021), totalling 58 unique samples after discarding poor quality libraries.…”

Section: Methodsmentioning

confidence: 99%

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Caste- and sex-specific DNA methylation in a bumblebee is associated with codon degeneracy

Marshall

Nicholas

Zweden

et al. 2021

Preprint

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Genomic imprinting is defined as parent-of-origin allele-specific expression. In order for genes to be expressed in this manner an `imprinting' mark must be present to distinguish the parental alleles within the genome. In mammals imprinted genes are primarily associated with DNA methylation. Genes exhibiting parent-of-origin expression have recently been identified in two species of Hymenoptera with functional DNA methylation systems; Apis mellifera and Bombus terrestris. We carried out whole genome bisulfite sequencing of parents and offspring from reciprocal crosses of two B. terrestris subspecies in order to identify parent-of-origin DNA methylation. We were unable to survey a large enough proportion of the genome to draw a conclusion on the presence of parent-of-origin DNA methylation however we were able to characterise the sex- and caste-specific methylomes of B. terrestris for the first time. We find males differ significantly to the two female castes, with differentially methylated genes involved in many histone modification related processes. We also analysed previously generated honeybee whole genome bisulfite data to see if genes previously identified as showing parent-of-origin DNA methylation in the honeybee show consistent allele-specific methylation in independent data sets. We have identified a core set of 12 genes in female castes which may be used for future experimental manipulation to explore the functional role of parent-of-origin DNA methylation in the honeybee. Finally, we have also identified allele-specific DNA methylation in honeybee male thorax tissue which suggests a role for DNA methylation in ploidy compensation in this species.

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“…(2021); Yi et al (2021) and Yagound et al (2020). We find around 1,500 genes per sample show allele-specific DNA methylation with slightly lower numbers in worker thorax tissue (Fig.…”

Section: Consistency Of Parent-of-origin Dna Methylation In the Honeybeementioning

confidence: 74%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Caste- and sex-specific DNA methylation in a bumblebee is associated with codon degeneracy

Marshall

Nicholas

Zweden

et al. 2021

Preprint

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“…All the queen cells with egg and larvae were kept in the same colony, and newly emerged queens were harvested immediately for weight. Some of emerged queens were kept in queen-less colonies for the number of ovarioles, the scoring ovariole number method was the same as previous report (Yi et al,2020), and 4 newly emerged queens in each group were kept in mating colonies and mated naturally. The rest of emerged queens were snap-frozen in liquid N 2 for queen thorax size measurement (queen thorax length, width, weight).…”

Section: Insects and Queen Rearingmentioning

confidence: 99%

“…In addition, worker bees will reform worker cell with egg or larva into queen cell and the egg or larva will develop to a new queen when the colony loss the old queen bee unexpectedly. Studies revealed that the quality of queens reared by young larvae were less favored than queens reared by eggs, and the quality of queens decreased with the reared larvae age (Woyle.,1971;Rangel.,2012;Yi et al,2020). Also, the growth of colonies with queens reared with older larvae is slower than that reared with younger larvae (Rangel.,2012).…”

Section: Introductionmentioning

confidence: 99%

Ovipositor sources for queen-rearing of honey bee (Apis mellifera) revealed inheritable maternal effect on reared queen

Yu¹,

Shi²,

He³

et al. 2021

Preprint

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Queen is arguably the most important member of a honey bee colony, and queen quality is crucial for honey bee colony growth and development. In this study, queens were reared with eggs laid in queen cells (QE), eggs laid in worker cells (WE) and 2-day old larvae in worker cells (L). Those physiological indexes (the weight, thorax size and number of ovarioles) of newly reared queens in each group were measured. Moreover, the reproductive potential of the newly reared queens and foraging ability of worker bees laid by the newly reared queens in each group were further explored. In addition, we also examined whether maternal effects would be transmitted to the offspring queens in honey bee. We found that the weight, number of ovarioles and thorax weight of newly emerged queens in QE were significantly higher than those in WE and L, suggesting the reproductive potential was stronger in QE group than WE and L group. Furthermore, offspring worker bees and queens of QE queens had higher weight at emergence than those from the other two groups. This study proved profound honey bee maternal effects on queen quality, which can be transmitted to their offspring. Our results of the present study were important for improving queen quality and promoting the development of beekeeping and agriculture.

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Honey bee (Apis cerana) queen rearing methods influence queen morphological characteristics and reproductive related gene expression

Zhong,

Ding,

Yuan

et al. 2024

Apidologie

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The quality of the queen is crucial for the survival and growth of honey bee colonies. There have been studies related to raising queens with excellent performance since the invention of artificial queen rearing technologies. However, relatively few studies have been undertaken that evaluate the relationship between queen quality and queen morphology and the expression level of reproduction-related genes using different-aged brood in artificial queen rearing practices of Eastern honey bees (Apis cerana). Therefore, we transferred eggs, 1-day-old larvae, 2-day-old larvae, and 3-day-old larvae to queen cells to rear queens until the queen emerged. We measured 12 indicators (the acceptance rate, birth weight, length, caliber and maximum diameter of queen cells, thorax length, width and weight, head width, wing length and width, and number of ovarian tubes). Moreover, the relative expression levels of Hexamerin, Vitellogenin, and Transferrin were measured using fat bodies and ovaries dissected from the newly emerged virgin queens. The results showed that queens reared from eggs exhibited the best morphological indexes and occupied the most abundant reproductive related gene expression level. Parameter values for queen from 1-day-old larvae were significantly higher than those for queens from 2-day-old larvae and 3-day-old larvae, and there was no significant difference between values for queens from 2-day-old larvae and 3-day-old larvae in most data, except for the relative gene expression. In conclusion, as the age of the brood increased, the quality of the queen bees declined. Rearing queens from fertilized eggs are far more likely to yield a better outcome for queen performance and colony function. This study provides data support for raising high-quality queens of Apis cerana, which would be benefit for the protection and better utilization of our native honey bee species.

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Effects of commercial queen rearing methods on queen fecundity and genome methylation

Cited by 9 publications

References 28 publications

Caste- and sex-specific DNA methylation in a bumblebee is associated with codon degeneracy

Caste- and sex-specific DNA methylation in a bumblebee is associated with codon degeneracy

Ovipositor sources for queen-rearing of honey bee (Apis mellifera) revealed inheritable maternal effect on reared queen

Honey bee (Apis cerana) queen rearing methods influence queen morphological characteristics and reproductive related gene expression

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