Effects of combustion-derived ultrafine particles and manufactured nanoparticles on heart cells in vitro

Helfenstein, Maria; Miragoli, Michele; Rohr, Stephan; Müller, Loretta; Wick, Peter; Mohr, Martin; Gehr, Peter; Rothen‐Rutishauser, Barbara

doi:10.1016/j.tox.2008.08.018

Cited by 64 publications

(52 citation statements)

References 47 publications

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“…The moderate effect of TiO 2 and DEP we observed here is consistent with their moderate effects on cardiomyocyte electrophysiology (Helfenstein et al 2008).…”

supporting

confidence: 89%

Biomechanical effects of environmental and engineered particles on human airway smooth muscle cells

Berntsen

Park²,

Rothen‐Rutishauser

et al. 2010

J. R. Soc. Interface.

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The past decade has seen significant increases in combustion-generated ambient particles, which contain a nanosized fraction (less than 100 nm), and even greater increases have occurred in engineered nanoparticles (NPs) propelled by the booming nanotechnology industry. Although inhalation of these particulates has become a public health concern, human health effects and mechanisms of action for NPs are not well understood. Focusing on the human airway smooth muscle cell, here we show that the cellular mechanical function is altered by particulate exposure in a manner that is dependent upon particle material, size and dose. We used Alamar Blue assay to measure cell viability and optical magnetic twisting cytometry to measure cell stiffness and agonist-induced contractility. The eight particle species fell into four categories, based on their respective effect on cell viability and on mechanical function. Cell viability was impaired and cell contractility was decreased by (i) zinc oxide (40 -100 nm and less than 44 mm) and copper(II) oxide (less than 50 nm); cell contractility was decreased by (ii) fluorescent polystyrene spheres (40 nm), increased by (iii) welding fumes and unchanged by (iv) diesel exhaust particles, titanium dioxide (25 nm) and copper(II) oxide (less than 5 mm), although in none of these cases was cell viability impaired. Treatment with hydrogen peroxide up to 500 mM did not alter viability or cell mechanics, suggesting that the particle effects are unlikely to be mediated by particle-generated reactive oxygen species. Our results highlight the susceptibility of cellular mechanical function to particulate exposures and suggest that direct exposure of the airway smooth muscle cells to particulates may initiate or aggravate respiratory diseases.

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“…The moderate effect of TiO 2 and DEP we observed here is consistent with their moderate effects on cardiomyocyte electrophysiology (Helfenstein et al 2008).…”

supporting

confidence: 89%

Biomechanical effects of environmental and engineered particles on human airway smooth muscle cells

Berntsen

Park²,

Rothen‐Rutishauser

et al. 2010

J. R. Soc. Interface.

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“…Also, CNTs did not promote an increase in oxidative stress, nor did they alter myofibrillar structure. 138 The same results were obtained by Ladeira et al, who used CNTs as siRNA transfection agents. 47 In 2010, Fung et al concluded that interactions of cultured cardiac myocytes with nanotubes were optimized when CNTs were vertically aligned (so the position is important for interactions to be established).…”

Section: -105supporting

confidence: 70%

Carbon nanotube interaction with extracellular matrix proteins producing scaffolds for tissue engineering

Tonelli¹,

Santos²,

Gomes³

et al. 2012

IJN

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Abstract:In recent years, significant progress has been made in organ transplantation, surgical reconstruction, and the use of artificial prostheses to treat the loss or failure of an organ or bone tissue. In recent years, considerable attention has been given to carbon nanotubes and collagen composite materials and their applications in the field of tissue engineering due to their minimal foreign-body reactions, an intrinsic antibacterial nature, biocompatibility, biodegradability, and the ability to be molded into various geometries and forms such as porous structures, suitable for cell ingrowth, proliferation, and differentiation. Recently, grafted collagen and some other natural and synthetic polymers with carbon nanotubes have been incorporated to increase the mechanical strength of these composites. Carbon nanotube composites are thus emerging as potential materials for artificial bone and bone regeneration in tissue engineering.

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“…Nowadays it is well documented that different cell types e.g. mesothelioma cells (MSTO-211H), primary neurons and glial cells, heart cells and co-cultures consisting of human epithelial lung cells (A549), human monocyte-derived macrophages, monocyte-derived dendritic cells (MDDC) are not severely affected when exposed to short SWCNT (Belyanskaya et al, 2009;Helfenstein et al, 2008;Kaiser et al, 2008;Muller et al, 2010;Wick et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Influence of single walled carbon nanotubes at subtoxical concentrations on cell adhesion and other cell parameters of human epithelial cells

Kaiser

Buerki-Thurnherr

Wick

2013

Journal of King Saud University - Science

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The production of SWCNT has increased tremendously in the last decade, due to high expectations of their benefits. Despite all precautions SWCNT may come into contact with organisms and may be taken up by them. The aim of the present study was to assess and elucidate the effects of SWCNT bundles (purified SWCNT) and SWCNT raw material on the behaviour of human epithelial cells (A549). When cells were exposed to SWCNT (bundles and raw material) cell adhesion was significantly affected. The effects of SWCNT were dependent on the SWCNT purity (purified SWCNT or SWCNT raw material), the SWCNT concentration and the incubation period.ª 2012 King Saud University. Production and hosting by Elsevier B.V. All rights reserved.Abbreviations: SWCNT, single walled carbon nanotubes; Total-FAK, focal adhesion kinase; p-FAK, phosphorylated focal adhesion kinase (activated FAK); Total-Erk1/2, mitogen-activated protein kinases; p-Erk1/2, phosphorylated mitogen-activated protein kinases (activated Erk1/2); ROS, Reactive oxygen species.

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Effects of combustion-derived ultrafine particles and manufactured nanoparticles on heart cells in vitro

Cited by 64 publications

References 47 publications

Biomechanical effects of environmental and engineered particles on human airway smooth muscle cells

Biomechanical effects of environmental and engineered particles on human airway smooth muscle cells

Carbon nanotube interaction with extracellular matrix proteins producing scaffolds for tissue engineering

Influence of single walled carbon nanotubes at subtoxical concentrations on cell adhesion and other cell parameters of human epithelial cells

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