Effects of combining Beauveria bassiana and Nosema pyrausta on the mortality of Ostrinia nubilalis

Rahman, K. M. Abdel; Barta, Marek; Cagáň, Ľudovít

doi:10.2478/s11535-010-0035-z

Cited by 9 publications

(5 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As the baiting insect G. mellonella belongs to Lepidoptera , isolates should have potential to infect it. Several previous studies (Abdel Rahman, Barta, & Cagáň, 2010; Demír, Secil, Demirbag, & Sevim, 2012) reported similar mortality; however, it depends on used strains as well as bioassay design. Safavi et al (2012) found average mortality for 10 7 conidia in ml in the range 12%–42% and highest mortality 57% after application of 10 8 dose.…”

Section: Resultsmentioning

confidence: 89%

“…Strain was able to overgrow 70% of cadavers by mycelia and sporulate when applied in dose 10 8 conidia in ml. Higher mortality can be achieved when fungus is applied together with another biocontrol agent like Bacillus thuringiensis (Wraight & Ramos, 2005) or N. pyrausta (Abdel Rahman et al, 2010) alternatively with sub‐lethal dose of chemical insecticide (Foschi & Grassi, 1985). In comparison with mentioned studies, our results indicate that SUAa83 can be good candidate for O. nubilalis regulation and will be subjected to field testing of efficiency to O. nubilalis .…”

Section: Resultsmentioning

confidence: 99%

Section: Re Sults and Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Variability in virulence of Beauveria spp. soil isolates against Ostrinia nubilalis

Medo

Medová

Michalko

et al. 2020

J Applied Entomology

Self Cite

View full text Add to dashboard Cite

European corn borer (Ostrinia nubilalis) is an important maize pest which is treated mainly by synthetic pesticides with many negative effects for ecosystem and human health. Soil borne entomopathogenic fungi of species belonging to Beauveria genus may provide an alternative strategy for biological control of this pest. In this article, we report results of virulence testing of 46 soil isolates of Beauveria spp. against O. nubilalis in relation to isolates origin and haplotype. All strains were collected from soil using Galleria mellonella bait method. Conidia suspension with concentration 10 7 conidia in ml was used for virulence test against 4th instar larvae of O. nubilalis at temperature 25°C. After 14 days, mortality of larvae was in the range 34%-96%. Median lethal time LT50 was estimated to 5.5-21.3 days. Mortality was not in relation to habitat or any other environmental parameter of sites where isolates came from. Differences among species were insignificant. Isolates were divided into 8 genetic lineages and 14 haplotypes using sequencing of ITS and Bloc nuclear regions. None of the lineages showed higher mortality than others. Despite more or less virulent isolates being found in each lineage, the three most virulent isolates belonged to the same lineage Beauveria bassiana A3. The four most virulent isolates were compared to commercial isolate GHA to assess medium lethal concentration. LC50 were estimated in range 7.5 × 10 4 to 9.5 × 10 5. Three of the four isolates showed equal or greater efficiency than strain GHA.

show abstract

Section: Resultsmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Section: Re Sults and Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Variability in virulence of Beauveria spp. soil isolates against Ostrinia nubilalis

Medo

Medová

Michalko

et al. 2020

J Applied Entomology

Self Cite

View full text Add to dashboard Cite

show abstract

“…When the combination of Beauveria bassiana and Nosema pyrausta (Paillot) Weiser c was used in laboratory, the mortality of the European corn borer larvae, Ostrinia nubilalis, increased significantly in all instars. Relative to the B. bassiana treatment alone, the B. bassiana + N. pyrausta treatment decreased the LC50s by 42.16 %, 37.63 %, 21.60 %, 27.11 % and 33.95 % for the first to the fifth instars, respectively (Rahman et al, 2010).…”

Section: Case Studies In Generalmentioning

confidence: 92%

Current Knowledge About Microorganisms Used in Biological Control of Pests and Their Relation to Bees: A Review

KŇAZOVICKÁ,

BENČAŤOVÁ,

STAROŇ

et al. 2022

Slovak J. Anim. Sci.

View full text Add to dashboard Cite

The aim of the review is to describe a control of pests by microorganisms and its possible effect on bees, other pollinators and beekeeping. Biological control seems to be a natural way to solve the problem with pests in agriculture as an alternative to the use of pesticides. However, the proposed solution must be closely associated with the safety to pollinators, which are an important part of plant production as well as forest ecosystems. Entomopathogenic bacteria (mainly bacilli) and entomopathogenic fungi (e.g., Beauveria bassiana and Metarhizium anisopliae) are often used to suppress the pests in agriculture. The application of entomopathogenic microsporidia is controversial because their frequent representatives are pollinators' pests. Moreover, biocontrol can be applied in the form of pollinator strips near the fields with monocultures resulting in plant and pollinator protection. In some countries, bees are also used as biovectors of control agents for the plant protection in the fields. On the other hand, specific pests pose a threat to bees themselves in the hives. Varoosis is a problem in beekeeping all over the world. The suppression of bee pests using microorganisms was tested. An activity of Beauveria bassiana against Varroa destructor shows promising results. Surprisingly, Beauveria bassiana can be isolated from cadavers of Galleria mellonella larvae, another bee pest, which destroy wax combs. Therefore, understanding of various links between the organisms could be helpful for sustainable beekeeping. Overall, humans are more conscious that everything is connected to each other. Protecting agents designed on natural basis often possess excellent results in practice. Therefore, testing them is more than desirable.

show abstract

“…B. bassiana is the most well studied entomopathogenic fungi and has been frequently used as a commercial mycoinsecticide in the feld [5,6]. For example, B. bassiana was used to control insect pests, including Pissodes castaneus, Ostrinia nubilalis, and Rhynchophorus ferrugineus, infeld through direct spraying [7][8][9]. It is known that B. bassiana is safe to human, natural benefcial insects, and benefcial microorganisms.…”

Section: Introductionmentioning

confidence: 99%

A Sensitive Method for Detecting Beauveria bassiana, an Insecticidal Biocontrol Agent, Population Dynamics, and Stability in Different Substrates

Gu,

Chen,

Zhang

et al. 2023

Canadian Journal of Infectious Diseases and Medical Microbiolog

View full text Add to dashboard Cite

Beauveria bassiana is a well-known insecticidal biocontrol agent. Despite its broad field applications, its survival, colonization, and stability under field conditions remained unclear, mainly due to the lack of a quick and reliable detection method. In this study, we developed a quantitative real-time PCR technology to monitor the stability and population dynamics of B. bassiana in different substrates (water, soil, and on the cotton leaves surface), different spores of B. bassiana applied on Chinese cabbage leaves surface, and the lethality of Pieris rapae spraying with different spores of B. bassiana. Our results showed a decreased concentration of B. bassiana DNA in all three substrates from the 1st day till 9th day of post inoculation (dpi) period, possibly due to the death of B. bassiana. After this decrease, a quick and significant rebound of B. bassiana DNA concentration was observed, starting from the 11th dpi in all three substrates. The B. bassiana DNA concentration reached the plateau at about 13th dpi in water and 17th dpi in the soil. On cotton leaves surface, the B. bassiana DNA concentration reached the highest level at the 17th dpi followed by a small decline and then stabilized. This increase of DNA concentration suggested recovery of B. bassiana growth in all three substrates. We found that the most suitable killing effectiveness of P. rapae was the 1.0 × 107 spores/mL of B. bassiana. In summary, we have established a detection technology that allows a fast and reliable monitoring for the concentration and stability of B. bassiana under different conditions. This technology can benefit and help us in the development of proper management strategies for the application of this biocontrol agent in the field.

show abstract

Effects of combining Beauveria bassiana and Nosema pyrausta on the mortality of Ostrinia nubilalis

Cited by 9 publications

References 39 publications

Variability in virulence of Beauveria spp. soil isolates against Ostrinia nubilalis

Variability in virulence of Beauveria spp. soil isolates against Ostrinia nubilalis

Current Knowledge About Microorganisms Used in Biological Control of Pests and Their Relation to Bees: A Review

A Sensitive Method for Detecting Beauveria bassiana, an Insecticidal Biocontrol Agent, Population Dynamics, and Stability in Different Substrates

Contact Info

Product

Resources

About