Effects of Combined Pulse Electromagnetic Field Stimulation Plus Glutamine on the Healing of Colonic Anastomosis in Rats

Gırgın, Sadullah; Gedik, Ercan; Öztürk, Hayrettin; Akpolat, Veysi; Kale, Ebru; Büyükbayram, Hüseyin; Çelik, Sait

doi:10.1007/s10620-008-0412-x

Cited by 6 publications

(8 citation statements)

References 32 publications

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“…Materials such as GLU, short-chain fatty acids, erythropoietin, fibrin glue and peritoneal graft have been experimentally reported to be beneficial in the healing of colonic anastomoses. [1][2][3]10,14,15 ARG is a dietary semi-essential amino acid and it is metabolized to ornithine and subsequently to proline and polyamines both known to interact in collagen synthesis. 4,16 Dietary ARG supplementation increases wound collagen accumulation.…”

Section: Discussionmentioning

confidence: 99%

“…8 It has the potential to stimulate wound healing, because one of the products of GLU metabolism is proline. 3 GLU enhances gut mucosal growth, repair, and improves intestinal injuries of animals and humans. 3 Postoperative GLU-enriched diet was shown to improve wound healing in rats.…”

Section: Discussionmentioning

confidence: 99%

“…1 The prevalence of AL varies in the literature between 0.5% and 30%. 2,3 Therefore, research has focused on various systemically or locally applied materials that can improve anastomotic healing. [1][2][3] Arginine (ARG) is a dietary semi essential amino acid that becomes conditionally indispensable in trauma and surgical patients.…”

mentioning

confidence: 99%

“…2,3 Therefore, research has focused on various systemically or locally applied materials that can improve anastomotic healing. [1][2][3] Arginine (ARG) is a dietary semi essential amino acid that becomes conditionally indispensable in trauma and surgical patients. 4,5 Dietary ARG supplementation induces positive nitrogen balance, increases hydroxyproline (HP) content, wound collagen accumulation, shows antioxidative effect and enhances wound healing.…”

mentioning

confidence: 99%

“…5,8 GLU is the most abundant amino acid, but its concentration falls after injury, surgery, or infection. 3 In various experimental and clinical studies, GLU had a positive effect on both wound and anastomotic healing. 9,10 Beta-hydroxy-beta-methylbutyrate (HMB) is a metabolite of the essential amino acid leucine.…”

mentioning

confidence: 99%

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The Effect of a Special Amino Acid Mixture on Healing of Left Colonic Anastomosis: an Experimental Study

Yaman¹,

Kara²,

Derıcı³

et al. 2013

Turkiye Klinikleri J Med Sci

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A AB BS ST TR RA AC CT T O Ob bj je ec ct ti iv ve e: : Arginine, glutamine, and Beta-hydroxy-beta-methylbutyrate were combined in a dietary supplement. This specialized amino acid mixture enhances wound collagen accumulation and increases wound healing. We aimed to investigate the effects of this special amino acid mixture on the healing process of experimental left colonic anastomosis. M Ma at te er ri ia al l a an nd d M Me et th h--o od ds s: : The study included 20 adult male Wistar-Albino rats. The study group (n=10) received 685 mg/kg/day specialized amino acid mixture for 7 days until 12 h before surgery and was maintained throughout the study. Following midline laparatomy a 1-cm segment of the left colon was resected. Bowel continuity was restored with an end-to-end anastomosis. The animals were re-anesthetized on day 7 after the operation and anastomotic bursting pressure was measured by passing a catheter per anum up to the area of anastomosis. Anastomotic segments were removed en bloc and were vertically divided into two. One was used for hydroxyproline measurement and the other for histopathological examination. Abramov's histologic scoring system was used in this study. R Re es su ul lt ts s: : The hydroxyproline levels and bursting pressures in the study group were superior compared to the control group. Collagen deposition and reepithelization scores of the study group were higher than that of the control group. C Co on nc cl lu us si io on n: Results of the present study indicates that significantly enhancing the anastomotic bursting pressure, hydroxyproline level and collagen deposition may improve anastomosis healing.K Ke ey y W Wo or rd ds s: : Colorectal surgery; arginine; glutamine; beta-hydroxyisovaleric acid Ö ÖZ ZE ET T A Am ma aç ç: : Arjinin, glutamin ve beta-hidroksi-beta-metilbütirat, diyet desteği olarak kombine edilmiştir. Bu özel amino asit karışımı, yara yerinde kollajen birikimini artırır ve yara iyileşmesini geliştirir. Bu çalışmanın amacı, deneysel sol kolon anastomozunun iyileşmesinde özel amino asit karışımının etkilerini araştırmaktır. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Çalışmada 20 adet yetişkin Wistar-Albino sıçan kullanıldı. Çalışma grubu (n=10) ameliyattan 7 gün öncesinden başlayarak tüm çalışma süresince 685 mg/kg/gün özel amino asit karışımı aldı. Orta hattan karın açıldı ve 1 cm'lik sol kolon segmenti rezeke edildi. Uç-uca anastomoz yapılarak barsak lümeninin devamlılığı sağlandı. Sıçanlara bu ameliyattan 7 gün sonra tekrar anestezi verildi. Anastomoz patlama basıncı, anüsten yerleştirilip anastomozun üzerine çıkan bir kateter ile ölçüldü. Anastomoz sahaları sağlam sınırlarla çıkarıldı ve dikey olarak iki parçaya bölündü. Bir parça hidroksiprolin ölçümünde, diğer parça histolojik değerlendirmede kullanıldı. Histolojik değerlendirme için Abramov'un skorlama sistemi kullanıldı. B Bu ul lg gu ul la ar r: : Hiroksiprolin düzeyleri ve patlama basınçları çalışma grubunda, kontrol grubuna göre daha yüksekti. Kollajen birikimi ve reepitelizasyon skorları ç...

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