Effects of Collagen Tripeptide Supplement on Photoaging and Epidermal Skin Barrier in UVB-exposed Hairless Mice

Pyun, Hee Bong; Kim, Minji; Park, Jieun; Sakai, Yasuo; Numata, Noriaki; Shin, Jin Yeong; Shin, Hyun Jung; Kim, Do Un; Hwang, Jae Kwan

doi:10.3746/pnf.2012.17.4.245

Cited by 92 publications

(70 citation statements)

References 26 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Repetitive UVB irradiation of hairless mice has been used previously to evaluate test compounds on skin photoageing, photodamage, inflammation and tumor development . UVB–induced erythema also has been used clinically in healthy volunteers to assess the photoprotective and anti‐inflammatory activity of potential therapeutic agents .…”

Section: Discussionmentioning

confidence: 99%

Reduction in ultraviolet B light–induced erythema by oxymetazoline and brimonidine is mediated by different α‐adrenoceptors

Hsia

Tian

Gil

2018

Experimental Dermatology

View full text Add to dashboard Cite

When applied topically, oxymetazoline and brimonidine reduce the persistent facial erythema of rosacea; this effect is mediated by cutaneous vasoconstriction induced by postsynaptic activation of α-adrenoceptors. We investigated the α-adrenergic pharmacology of oxymetazoline and brimonidine. Functional activity on α-adrenoceptors was evaluated in vitro in HEK293 cells stably expressing single receptor subtypes using a fluorometric imaging plate reader Ca influx assay. Oxymetazoline was an α -adrenoceptor agonist with partial α -adrenoceptor activity, whereas brimonidine was a highly selective full α -adrenoceptor agonist. In vivo pharmacology was investigated in a mouse model of ultraviolet B light (UVB)-induced skin erythema. To selectively inhibit α-adrenoceptor subtypes, mice were injected with prazosin (an α -selective antagonist) or rauwolscine (an α -selective antagonist) following UVB exposure. Oxymetazoline cream 1.0%, brimonidine gel 0.33% or vehicle control was applied topically, and erythema was measured using a chromameter. Oxymetazoline and brimonidine reduced UVB-induced erythema compared with vehicle control (P < .01). The effect of oxymetazoline was impaired in prazosin-pretreated but not rauwolscine-pretreated mice. Conversely, the effect of brimonidine was impaired in rauwolscine-pretreated but not prazosin-pretreated mice. These data suggest that while oxymetazoline and brimonidine produce cutaneous vasoconstriction, they do so through different α-adrenergic mechanisms, with oxymetazoline primarily acting via α -adrenoceptors and brimonidine acting via α -adrenoceptors.

show abstract

Section: Discussionmentioning

confidence: 99%

Reduction in ultraviolet B light–induced erythema by oxymetazoline and brimonidine is mediated by different α‐adrenoceptors

Hsia

Tian

Gil

2018

Experimental Dermatology

View full text Add to dashboard Cite

show abstract

“…Also, collagen hydrolysates from Chum Salmon skin were found to stimulate collagen synthesis and decrease collagen degradation in a dose-dependent manner [7]. Moreover, Gly-X-Y tripeptides derived from collagen hydrolysates also showed anti-aging properties such as increased skin moisture content, increased skin elasticity, and decreased MMP activities in UV-irradiated hairless mice [12].…”

Section: Introductionmentioning

confidence: 97%

Anti-aging potential of fish collagen hydrolysates subjected to simulated gastrointestinal digestion and Caco-2 cell permeation

Han

Lee

et al. 2019

JABC

View full text Add to dashboard Cite

The objectives of this study were to evaluate the antiaging effects and investigate the effect of simulated gastrointestinal (GI) digestion on the anti-aging properties and intestinal permeation of the potential fish collagen hydrolysates (FCH). Therefore, procollagen synthesis, matrix metalloproteinase-1 (MMP-1) production, and Caco-2 cell permeability were analyzed before and after in vitro digestion for FCHs, low-molecular weight fractions (<1 kDa), and high molecular weight fractions (>1 kDa). After being subjected to GI digestion, the level of MMP-1 inhibition was maintained, although the procollagen production was significantly (>20%) lower with all samples. Also, the digested FCHs and their <1 kDa fraction yielded 9.1 and 13.8% increased peptide transport, respectively, compared to undigested samples. Based on the effective intestinal permeation and high digestive enzyme stability, the <1 kDa fraction of FCHs is a potential bioactive material suitable for anti-aging applications in the food and cosmetics industries.

show abstract

“…These CTP are produced in several sequences, including Gly-Pro-Hyp, Gly-Pro-Ala, and Gly-Ala-Hyp. CTP can help in healing bone 16,17) and has several beneficial effects on the skin, including protection against photoaging caused by UV-B irradiation 18) and anti-wrinkle and anti-pruritus effects, 19) and enhancing hyaluronic acid synthesis. 19) In addition, a hydrolysate of chicken collagen peptides rich in Gly-Pro-Hyp can affect osteoporosis.…”

mentioning

confidence: 99%

Absorption and plasma kinetics of collagen tripeptide after peroral or intraperitoneal administration in rats

Yamamoto¹,

Hayasaka²,

Deguchi³

et al. 2015

Bioscience, Biotechnology, and Biochemistry

Self Cite

View full text Add to dashboard Cite

Collagen tripeptide (CTP) is a collagen-derived compound containing a high concentration of tripeptides with a Gly-X-Y sequence. In this study, the concentrations and metabolites of CTP were monitored in rat plasma after its administration. We performed a quantitative analysis using high-performance liquid chromatography tandem mass spectrometry according to the isotopic dilution method with stable isotopes. We confirmed that the tripeptides Gly-Pro-Hyp, Gly-Pro-Ala, and Gly-Ala-Hyp were transported into the plasma. Dipeptides, which are generated by degradation of the N- or C-terminus of the tripeptides Gly-Pro-Hyp, Gly-Pro-Ala, and Gly-Ala-Hyp, were also present in plasma. The plasma kinetics for peroral and intraperitoneal administration was similar. In addition, tripeptides and dipeptides were detected in no-administration rat blood. The pharmacokinetics were monitored in rats perorally administered with Gly-[(3)H]Pro-Hyp. Furthermore, CTP was incorporated into tissues including skin, bone, and joint tissue. Thus, administering collagen as tripeptides enables efficient absorption of tripeptides and dipeptides.

show abstract

Effects of Collagen Tripeptide Supplement on Photoaging and Epidermal Skin Barrier in UVB-exposed Hairless Mice

Cited by 92 publications

References 26 publications

Reduction in ultraviolet B light–induced erythema by oxymetazoline and brimonidine is mediated by different α‐adrenoceptors

Reduction in ultraviolet B light–induced erythema by oxymetazoline and brimonidine is mediated by different α‐adrenoceptors

Anti-aging potential of fish collagen hydrolysates subjected to simulated gastrointestinal digestion and Caco-2 cell permeation

Absorption and plasma kinetics of collagen tripeptide after peroral or intraperitoneal administration in rats

Contact Info

Product

Resources

About