Effects of Cold Stratification and Ga3 on Germination Ofarbutus Unedoseeds of Three Provenances

Pipinis, Elias; Stampoulidis, Athanasios; Milios, Elias; Kitikidou, Kyriaki; Radoglou, K.

doi:10.21010/ajtcam.v14i1.34

Cited by 7 publications

(5 citation statements)

References 8 publications

(10 reference statements)

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“…In this study, the germination percentages of the half seeds and scarified seeds were significantly higher than those of the intact seeds (Figure 3), suggesting that E. ulmoides seeds likely have both PY and a shallow PD. These results were consistent with those of Yang et al [13,34]. According to the imbibition curve, from four hours of imbibition on, the water absorption of both the scarified seeds and the isolated embryos was significantly higher than the intact seeds (Figure 4).…”

Section: Determination Of the Dormancy Typesupporting

confidence: 91%

Effects of Temperature, Scarification, Stratification, Phytohormones, and After-Ripening on the Dormancy and Germination of Eucommia ulmoides Oliv. Seeds

Deng

Zhou

Wang

et al. 2021

Forests

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Eucommia ulmoides Oliv., the only member of the family Eucommiaceae, is endemic to China and has great development and utilization prospects. The seeds of E. ulmoides show dormancy but the underlying mechanism remains unknown. The aim of this study was to determine the cause of the seed dormancy and provide fundamental knowledge for the breeding, genetic improvement, and conservation of the germplasm resources of this species. According to the seed dormancy classification system developed by Jerry M. Baskin and Carol C. Baskin, we compared the germination percentage between intact seeds and isolated embryos, constructed water absorption curves, and evaluated the germination of seeds treated with scarification, cold/warm-moist stratification, after-ripening during dry storage, and gibberellic acid (GA3). The results showed that the intact seeds germinated only at 10 °C with a low germination percentage of 13.3% whereas the isolated embryos had a high normal germination percentage among a wider range of temperatures. According to the results from the scarified seeds, half seeds, and intact seeds, the seed coat significantly restricted the embryo water absorption. The scarification, after-ripening, cold/warm-moist stratification, and GA3 treatments promoted seed germination. Among them, cold-moist stratification was the most effective method and the temperature range of seed germination increased in both directions from 10 °C with prolonged stratification. The germination percentage increased significantly at constant temperatures with the highest germination percentage of 93.7 ± 0.3% at 10 °C and a light/dark cycle after 90 days of cold-moist stratification. Therefore, the freshly harvested E. ulmoides seeds exhibited a combinational dormancy comprising physical and Type 3 non-deep physiological dormancy, causing limited embryo water absorption by the seed coat and a low embryo growth potential. Given the unique phylogenetic characteristics and utility of E. ulmoides, our findings should promote studies of seed dormancy evolution and the development and application of E. ulmoides germplasm resources.

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Section: Determination Of the Dormancy Typesupporting

confidence: 91%

Effects of Temperature, Scarification, Stratification, Phytohormones, and After-Ripening on the Dormancy and Germination of Eucommia ulmoides Oliv. Seeds

Deng

Zhou

Wang

et al. 2021

Forests

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“…According to Ricardo and Veloso (1987), about 90% and 50% of Arbutus unedo seeds treated only with 500 ppm GA3 germinated at 20°C and 25°C, respectively. In a study by Pipinis et al (2017), non-stratified seeds of three Arbutus unedo varieties that were not treated with GA3 solutions exhibited very low germination (0.83-7.50%). In the present study, like the above-mentioned studies, the highest germination percentage was observed with 500 ppm GA3 treatment, while the lowest germination was observed in the control group (without GA3 addition).…”

Section: Discussionmentioning

confidence: 98%

“…However, a major constraint to the sexual propagation of many species is the poor germination of their seeds. This is possibly due to low viability, although it is frequently due to seed dormancy (Mackay et al 2002, Pipinis et al 2017. Seed dormancy is a physiological condition in which the seeds cannot germinate even under the most suitable germination conditions that are in an environment with suitable temperatures, humidity and light conditions, or do not exhibit uniform germination.…”

Section: Introductionmentioning

confidence: 99%

The Effects of GA3 and Storage Time on the Germination of Epigaea gaultherioides (Ericaceae) Seeds

et al. 2022

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Turkey is a country with diverse plant species and has significant potential in landscape planning. Epigaea gaultherioides (Boisse et Bal.) Takht. is a relic of the Black Sea (Euxine) province, aesthetic, and functional species indigenous to Northeastern Turkey and under the threat of extinction. The species should be cultured to ensure the permanence of the species with ex situ methods and employed in landscape planting design. The present study aimed to determine the effects of gibberellic acid (GA3) and cold storage time on seed germination performance in E. gaultherioides. Cold storage was applied to the seeds at +4 ºC for 16 and 28 months and subsequently the seeds were soaked in GA3 (0, 100, 500 and 1,000 ppm) solutions for 24 hours. The findings indicated that E. gaultherioides seeds that were not treated with GA3 solutions did not germinate. However, it was determined that germination was low in seeds treated with 500 ppm GA3, and 80% of the seeds germinated when treated with cold storage at 25 °C (24 h dark) for 16 months. The ANOVA revealed that there were statistically significant differences between germination percentage and mean germination times for various gibberellic acid doses and cold storage – time processes. Keywords: cold storage time, germination rate, gibberellic acid, landscape, relict

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“…Cold stratification treatment is an effective method for woody plants to alleviate seed dormancy [37], so it can be used to solve the physiological dormancy of Tilia seeds [38]. And GA 3 treatment can release seed dormancy early and shorten the duration of cold stratification [39]. Combined with magnetic field treatment, the germination time of most seeds was effectively advanced by 15 days.…”

Section: Discussionmentioning

confidence: 99%

The Impact of Magnetic Field and Gibberellin Treatment on the Release of Dormancy and Internal Nutrient Transformation in Tilia miqueliana Maxim. Seeds

Shi,

Cao,

Gao

et al. 2024

Forests

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The seeds of Tilia miqueliana Maxim. exhibit deep dormancy, which is categorized as combinational dormancy. This study utilized a comprehensive treatment involving magnetic fields, gibberellin (GA3), and cold stratification to promote the release of seed physiological dormancy and enhance germination rates. After being soaked in 98% H2SO4 for 15 min, mature seeds of Tilia were exposed to magnetic field treatments (150 MT, 250 MT) for different durations (25 min, 45 min, 65 min, and 85 min), as well as GA3 solution soaking (concentration: 0 μmol·L−1, 1443 μmol·L−1). Subsequently, cold stratification (0–5 °C) was applied to investigate the effects of these treatments on seed dormancy release and nutrient transformation. The results indicated that the comprehensive treatment involving magnetic field, GA3 solution soaking, and cold stratification effectively released the physiological dormancy of Tilia seeds and improved germination rates. Among the treatments, M150T85G1443 (magnetic field intensity: 150 MT, magnetic field treatment time: 85 min, GA3 soaking concentration: 1433 μmol·L−1) exhibited the most favorable outcome. After 75 days of cold stratification following the comprehensive treatments, the germination rate of M150T85G1443 seeds reached 89%. Additionally, the levels of storage substances such as starches and crude fats within the seeds decreased, while the utilization of soluble sugars and soluble proteins increased. The M150T85G1443 treatment exhibited the highest degree of variation, leading to gradual increases in metabolic activities of the seeds and a transition from dormancy to germination.

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Effects of Cold Stratification and Ga3 on Germination Ofarbutus Unedoseeds of Three Provenances

Cited by 7 publications

References 8 publications

Effects of Temperature, Scarification, Stratification, Phytohormones, and After-Ripening on the Dormancy and Germination of Eucommia ulmoides Oliv. Seeds

Effects of Temperature, Scarification, Stratification, Phytohormones, and After-Ripening on the Dormancy and Germination of Eucommia ulmoides Oliv. Seeds

The Effects of GA3 and Storage Time on the Germination of Epigaea gaultherioides (Ericaceae) Seeds

The Impact of Magnetic Field and Gibberellin Treatment on the Release of Dormancy and Internal Nutrient Transformation in Tilia miqueliana Maxim. Seeds

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