Effects of cold-inducible RNA-binding protein on the proliferation and apoptosis of spermatogenic cells in vitro following heat stress

Cheng, Guiping; Ke, Dandan; Rao, Meng; Hu, Song; Wang, Yingying; Zhou, Fang; Liu, Heyu; Zhu, Changhong; Xia, Wei

doi:10.1071/rd18469

Cited by 9 publications

(14 citation statements)

References 35 publications

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“…These findings were consistent with those obtained in the testicular tissue of mice. As in our previous study, 28 the proliferation of GC2 cells was inhibited after heat treatment. We found that the inhibition of miR‐128‐3p expression in GC2 cells increased p‐MAPK14 expression, inhibited cell proliferation, and increased the cell apoptosis rate, consistent with the findings for heat‐treated GC2 cells.…”

Section: Discussionsupporting

confidence: 80%

“…GC‐2‐spd, the mouse spermatocyte cell line, was obtained from American Type Culture Collection. As described in our previous research, 28 the cells were cultured in Dulbecco's Modified Eagle Medium (Gibco), supplemented with 10% fetal bovine serum and 100 U/mL penicillin and streptomycin, at 37℃ in a humidified atmosphere containing 5% CO2 until an appropriate density was obtained. They were then transferred to a 43℃ incubator for 1 h. Subsequently, the cells were returned to the incubator at 37℃ for further culture.…”

Section: Methodsmentioning

confidence: 99%

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The role of miR‐128‐3p through MAPK14 activation in the apoptosis of GC2 spermatocyte cell line following heat stress

Zou

Cheng

et al. 2020

Andrology

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Background MicroRNAs play a crucial role in the regulation of spermatogenesis. For example, miR‐128‐3p expression is known to decrease significantly after testicular hyperthermia, but the regulatory effect of this change on the spermatogenesis damage caused by heat stress remains unclear. Objectives This study aimed to verify whether the target gene of miR‐128‐3p is MAPK14, which affects spermatogenic cell proliferation and apoptosis under testicular hyperthermia. Materials and methods Mouse testis and GC2 spermatocyte cell line heat stress models were established. miR‐128‐3p expression before and after heat stress was analyzed by reverse transcription polymerase chain reaction. MAPK14 and p‐MAPK14 expression was detected by Western blot, and cell apoptosis was analyzed by Annexin V‐FITC/PI. Subsequently, miR‐128‐3p inhibitors and mimics were used to interfere with spermatocytes before and after heat stress, respectively, for correlation detection. Results Compared with the control group, the heat stress group showed decreased miR‐128‐3p expression, increased p‐MAPK14 expression, and decreased cell proliferation activity. In the GC2‐spd cell line in vitro, miR‐128‐3p inhibitors were found to upregulate p‐MAPK14 expression, reduce cell proliferation activity, and increase apoptosis, consistent with the results obtained in the heat treatment alone. Furthermore, miR‐128‐3p mimics transfected in the GC2 cells after heat stress reduced p‐MAPK14 expression, alleviated the decrease in cell proliferation, and decreased the apoptosis level. Conclusions The downregulation of miR‐128‐3p expression plays an important role in spermatogenesis damages after testicular hyperthermia, which is probably attributable to the activation of the MAPK signaling pathway. Downregulated miR‐128‐3p expression induces the apoptosis and inhibits the proliferation of spermatogenic cells by promoting MAPK14 phosphorylation.

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Section: Discussionsupporting

confidence: 80%

Section: Methodsmentioning

confidence: 99%

The role of miR‐128‐3p through MAPK14 activation in the apoptosis of GC2 spermatocyte cell line following heat stress

Zou

Cheng

et al. 2020

Andrology

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“…For example, the genes encoding the cold inducible RNA binding proteins ( cirbpa and cirbpb ) and the high mobility group box proteins ( hmgb1b and hmgb3a ) were regarded as marker genes for cold stress ( Gracey et al, 2004 ; Long et al, 2013 ). CIRBP is a stress-responsive protein has protective effects against tissue injury and cell apoptosis caused by both hyperthermia and hypothermia stressors ( Yu et al, 2018 ; Cheng et al, 2019 ; Liu et al, 2019 ). HMGB1 is the best characterized damage-associated molecular pattern, which is normally inside the cell and is released after cell death or secreted upon severe stresses.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional Programs Underlying Cold Acclimation of Common Carp (Cyprinus carpio L.)

Long

et al. 2020

Front. Genet.

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Properly regulated transcriptional responses to environmental perturbations are critical for the fitness of fish. Although gene expression profiles in the tissues of common carp upon cold stress were previously characterized, the transcriptional programs underlying cold acclimation are still not well known. In this study, the ability of three common carp strains including Hebao red carp (HB), Songpu mirror carp (SPM) and Yellow river carp (YR) to establish cold resistance after acclimation to a mild hypothermia stress at 18°C for 24 h was confirmed by measurements of the critical thermal minimums (CTMin). The gene expression profiles of the brain and the heart from these strains under both control and cold-acclimated conditions were characterized with RNA-sequencing. The data of the three common carp strains with different genetic background were combined in the differential gene expression analyses to balance the effects of genetic diversity on gene expression. Marked effects of tissue origins on the cold-induced transcriptional responses were revealed by comparing the differentially expressed gene (DEG) lists of the two tissues. Functional categories including spliceosome and RNA splicing were highly enriched in the DEGs of both tissues. However, steroid biosynthesis was specifically enriched in DEGs of the brain and response to unfolded protein was solely enriched in DEGs of the heart. Consistent with the up-regulation of the genes involved in cholesterol biosynthesis, total cholesterol content of the brain was significantly increased upon cold stress. Moreover, cold-induced alternative splicing (AS) events were explored and AS of the rbmx (RNA-binding motif protein, X chromosome) gene was confirmed by real-time quantitative PCR. Finally, a core set of cold responsive genes (CRGs) were defined by comparative transcriptomic analyses. Our data provide insights into the transcriptional programs underlying cold acclimation of common carp and offer valuable clues for further investigating the genetic determinants for cold resistance of farmed fish.

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“…Accumulated evidence reveals that Cirbp has been implicated in different physiological and pathological processes, including cell proliferation and differentiation, cell senescence, cell survival and apoptosis, oxidative stress, DNA damage and repair, immune and in ammatory responses, telomere maintenance, circadian rhythm, spermatogenesis, and tumor formation and progression, etc [15][16][17][19][20][21][22][23]. Of note, several lines of evidence has indicated that Cirbp is associated with cell stresses, including heat and cold [24][25][26][27][28][29]. Chronic hypoxia-induced Cirbp hypermethylation attenuated hypothermic cardioprotection via down-regulation of ubiquinone biosynthesis [24].…”

mentioning

confidence: 99%

“…Therapeutic hypothermia protected photoreceptors through activating Cirbp pathway [25]. The down-regulation of Cirbp expression was observed in male germ cells of mice and humans under heat stress condition [26][27][28]. Moreover, in prostate cancer cells, heat treatment upregulated heart shock proteins and down-regulated cold shock proteins [i.e., Cirbp and RNA binding motif protein 3 (RBM3)] [29].…”

mentioning

confidence: 99%

ThermomiR-377-3p-induced Suppression of Cirbp Expression Is Required for Effective Elimination of Cancer Cells and Cancer Stem-like Cells by Hyperthermia

Lin

Jia

Luo

et al. 2021

Preprint

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In recent years, the development of adjunctive therapeutic hyperthermia for cancer therapy has received considerable attention. However, the mechanisms underlying hyperthermia resistance are still poorly understood. In this study, we investigated the roles of cold-inducible RNA binding protein (Cirbp) in regulating hyperthermia resistance and underlying mechanisms in nasopharyngeal carcinoma (NPC). Our results firstly revealed that hyperthermia significantly attenuated the stemness property of NPC cells, while combination treatment of hyperthermia and oridonin dramatically increased the killing effect on NPC cells and cancer stem cell (CSC)-like population. Moreover, hyperthermia substantially improved the sensitivity of radiation-resistant NPC cells and CSC-like cells to radiotherapy. Hyperthermia noticeably suppressed Cirbp expression in NPC cells and xenograft tumor tissues. Furthermore, Cirbp inhibition remarkably boosted anti-tumor-killing activity of hyperthermia against NPC cells and CSC-like cells, whereas ectopic expression of Cirbp compromised tumor-killing effect of hyperthermia on these cells, indicating that Cirbp overexpression induces hyperthermia resistance. ThermomiR-377–3p improved the sensitivity of NPC cells and cancer stem-like cells to hyperthermia in vitro by directly suppressing Cirbp expression. More importantly, our results displayed the significantly boosted sensitization of tumor xenografts to hyperthermia by Cirbp silencing in vivo, but ectopic expression of Cirbp nearly completely counteracted hyperthermia-mediated tumor cell-killing effect against tumor xenografts in vivo. Mechanistically, Cirbp silencing-induced inhibition of DNA damage repair by inactivating ATM-Chk2 and ATR-Chk1 pathways, decrease in stemness and increase in cell death contributed to hyperthermic sensitization; conversely, Cirbp overexpression-induced promotion of DNA damage repair, increase in stemness and decrease in cell apoptosis contributed to hyperthermia resistance. Taken together, these findings reveal a previously unrecognized role for Cirbp in positively regulating hyperthermia resistance and suggest that thermomiR-377–3p and its target gene Cirbp represent promising targets for therapeutic hyperthermia.

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Effects of cold-inducible RNA-binding protein on the proliferation and apoptosis of spermatogenic cells in vitro following heat stress

Cited by 9 publications

References 35 publications

The role of miR‐128‐3p through MAPK14 activation in the apoptosis of GC2 spermatocyte cell line following heat stress

The role of miR‐128‐3p through MAPK14 activation in the apoptosis of GC2 spermatocyte cell line following heat stress

Transcriptional Programs Underlying Cold Acclimation of Common Carp (Cyprinus carpio L.)

ThermomiR-377-3p-induced Suppression of Cirbp Expression Is Required for Effective Elimination of Cancer Cells and Cancer Stem-like Cells by Hyperthermia

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