Effects of co-expressing the plant CDK inhibitor ICK1 and D-type cyclin genes on plant growth, cell size and ploidy in Arabidopsis thaliana

Zhou, Yongming; Wang, Hong; Gilmer, Susan; Whitwill, Steve; Fowke, Larry C.

doi:10.1007/s00425-002-0935-x

Cited by 77 publications

(34 citation statements)

References 51 publications

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“…Collectively, these results show that over-expression of the ICK/KRP CDK inhibitors can affect plant growth and also morphology. It should be noted that the phenotypic effects depend not only on the expression of CDK inhibitors but also the expression of interacting regulators such as D-type cyclins (Jasinski et al, 2002b;Zhou et al, 2003b). From a cell biology point of view, it is interesting to consider the effects of ICK/KRPs on important cellular processes.…”

Section: Ick/krp Cdk Inhibitors and Cellular Processes In Plantsmentioning

confidence: 99%

Functions, regulation and cellular localization of plant cyclin‐dependent kinase inhibitors

Wang

Zhou

Bird

et al. 2008

Journal of Microscopy

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SummaryThe cell cycle is regulated by the cyclin-dependent kinase (CDK), and CDK inhibitors can bind to CDKs and inhibit their activities. This review examines plant CDK inhibitors, with particular emphasis on their molecular and cellular functions, regulation and cellular localization. In plants, a family of ICK/KRP CDK inhibitors represented by ICK1 is known and another type of CDK inhibitor represented by the SIMESE (SIM) has recently been reported. Considerable understanding has been gained with the ICK/KRP CDK inhibitors. These plant CDK inhibitors share only limited sequence similarity in the Cterminal region with the KIP/CIP family of mammalian CDK inhibitors. The ICK/KRP CDK inhibitors thus provide good tools to understand the basic machinery as well as the unique aspects of the plant cell cycle. The ICK/KRP CDK inhibitors interact with D-type cyclins or A-type CDKs or both. Several functional regions and motifs have been identified in ICK1 for CDK inhibition, nuclear localization and protein instability. Clear evidence shows that ICK/KRP proteins are important for the cell cycle and endoreduplication. Preliminary evidence suggests that they may also be involved in cell differentiation and cell death. Results so far show that plant CDK inhibitors are exclusively localized in the nucleus. The molecular sequences regulating the localization and functional significance will be discussed.

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Section: Ick/krp Cdk Inhibitors and Cellular Processes In Plantsmentioning

confidence: 99%

Functions, regulation and cellular localization of plant cyclin‐dependent kinase inhibitors

Wang

Zhou

Bird

et al. 2008

Journal of Microscopy

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“…CDKB1;1 , a key regulatory gene in the G2-M phase transition, has been shown to negatively regulate the onset of endocycle4. High expression level of ICK/KRP1 may suppress the endocycle and inhibit G2-M phase transition4565. RBR1 regulates the switch from proliferation to endocycles by targeting the CCS52A1 and CSS52A2 genes33.…”

mentioning

confidence: 99%

AtDOF5.4/OBP4, a DOF Transcription Factor Gene that Negatively Regulates Cell Cycle Progression and Cell Expansion in Arabidopsis thaliana

Chen

et al. 2016

Sci Rep

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In contrast to animals, plant development involves continuous organ formation, which requires strict regulation of cell proliferation. The core cell cycle machinery is conserved across plants and animals, but plants have developed new mechanisms that precisely regulate cell proliferation in response to internal and external stimuli. Here, we report that the DOF transcription factor OBP4 negatively regulates cell proliferation and expansion. OBP4 is a nuclear protein. Constitutive and inducible overexpression of OBP4 reduced the cell size and number, resulting in dwarf plants. Inducible overexpression of OBP4 in Arabidopsis also promoted early endocycle onset and inhibited cell expansion, while inducible overexpression of OBP4 fused to the VP16 activation domain in Arabidopsis delayed endocycle onset and promoted plant growth. Furthermore, gene expression analysis showed that cell cycle regulators and cell wall expansion factors were largely down-regulated in the OBP4 overexpression lines. Short-term inducible analysis coupled with in vivo ChIP assays indicated that OBP4 targets the CyclinB1;1, CDKB1;1 and XTH genes. These results strongly suggest that OBP4 is a negative regulator of cell cycle progression and cell growth. These findings increase our understanding of the transcriptional regulation of the cell cycle in plants.

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“…The interactions between ICK ⁄ KRP and D-type cyclins were also observed in twohybrid targeted screens (Wang et al, 1998;Jakoby et al, 2006). Furthermore the in planta interaction was indirectly demonstrated by the simultaneous overexpression of AtCycD3;1 and NiKIS1a from tobacco, as a wild-type leaf phenotype was restored in the double overexpressing plants (Jasinski et al, 2002b;Schnittger et al, 2003;Zhou et al, 2003a). In addition, SlKRP1 is able to import SlCDKA;1 into the nucleus (Fig.…”

Section: Slkrp1 Interaction With Cdka;1 and Cycd3;1mentioning

confidence: 86%

Functional characterization of the tomato cyclin‐dependent kinase inhibitor SlKRP1 domains involved in protein–protein interactions

et al. 2010

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Summary• Cyclin-dependent kinase (CDK) inhibitors (kip-related proteins, KRPs) play a major role in the regulation of plant cell cycle in antagonizing its progression, and are thus regulators of development. The primary sequence of KRPs is characterized by the existence of conserved motifs, for which we have limited information on their functional significance.• We performed a functional analysis of various domains present in KRPs from tomato. A series of deletion mutants of SlKRP1 was generated and used in transient expression assays to define the relevance of conserved protein domains in subcellular and subnuclear localizations. Specific interactions of SlKRP1 and its deletion variants with cell cycle proteins were investigated using two-hybrid assays and bimolecular fluorescent complementation.• Plant KRPs are distributed into two phylogenetic subgroups according to the presence of conserved motifs. Members of subgroup 1 represented by SlKRP1 share 6 conserved motifs whose function in protein localization and proteinprotein interactions could be identified. A new interaction motif was localized in the central part of SlKRP1 that targets SlCDKA1 and SlCYCD3;1 to the nucleus.• Our results bring new insights to the functional role of particular domains in KRPs relative to subcellular localization or proteolytic degradation.

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Effects of co-expressing the plant CDK inhibitor ICK1 and D-type cyclin genes on plant growth, cell size and ploidy in Arabidopsis thaliana

Cited by 77 publications

References 51 publications

Functions, regulation and cellular localization of plant cyclin‐dependent kinase inhibitors

Functions, regulation and cellular localization of plant cyclin‐dependent kinase inhibitors

AtDOF5.4/OBP4, a DOF Transcription Factor Gene that Negatively Regulates Cell Cycle Progression and Cell Expansion in Arabidopsis thaliana

Functional characterization of the tomato cyclin‐dependent kinase inhibitor SlKRP1 domains involved in protein–protein interactions

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