Effects of co-composting of lincomycin mycelia dregs with furfural slag on lincomycin degradation, degradation products, antibiotic resistance genes and bacterial community

Ren, Shengtao; Lu, Aqian; Guo, Xiaoying; Zhang, Qianqian; Wu, Yan; Guo, Xinwu; Wang, Lianzhong; Zhang, Baobao

doi:10.1016/j.biortech.2018.10.014

Cited by 43 publications

(6 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These two retention times suggest that there are two isomers of TP423 being formed during comammox biotransformation. A molecular ion of m/z 423 with two domain fragment ions (m/z = 126.13 and 359.21) was previously identified in the co-composing of LIN mycelia dregs as LIN's hydroxylation at the methylthio group ( Calza et al, 2012 ;Ren et al, 2019 ). According to the fragment ions, TP423 at 1.75 min in this study might have been generated from LIN hydroxylation at the methylthio group or S-oxidation of LIN ( Spizek and Rezanka, 2017 ) ( Table 1 ), and TP423 at 2.8 min might have been obtained by hydroxylation of the methyl group of the carboxamide moiety ( Table 1 and Fig.…”

Section: Transformation Product Identification Of Lin and Fqsmentioning

confidence: 99%

Biotransformation of lincomycin and fluoroquinolone antibiotics by the ammonia oxidizers AOA, AOB and comammox: A comparison of removal, pathways, and mechanisms

et al. 2021

View full text Add to dashboard Cite

In this study, we evaluated the biotransformation mechanisms of lincomycin (LIN) and three fluoroquinolone antibiotics (FQs), ciprofloxacin (CFX), norfloxacin (NFX), and ofloxacin (OFX), which regularly enter aquatic environments through human activities, by different ammonia-oxidizing microorganisms (AOM). The organisms included a pure culture of the complete ammonia oxidizer (comammox) Nitrospira inopinata , an ammonia oxidizing archaeon (AOA) Nitrososphaera gargensis , and an ammonia-oxidizing bacterium (AOB) Nitrosomonas nitrosa Nm90. The removal of these antibiotics by the pure microbial cultures and the protein-normalized biotransformation rate constants indicated that LIN was significantly co-metabolically biotransformed by AOA and comammox, but not by AOB. CFX and NFX were significantly co-metabolized by AOA and AOB, but not by comammox. None of the tested cultures transformed OFX effectively. Generally, AOA showed the best biotransformation capability for LIN and FQs, followed by comammox and AOB. The transformation products and their related biotransformation mechanisms were also elucidated. i) The AOA performed hydroxylation, S-oxidation, and demethylation of LIN, as well as nitrosation and cleavage of the piperazine moiety of CFX and NFX; ii) the AOB utilized nitrosation to biotransform CFX and NFX; and iii) the comammox carried out hydroxylation, demethylation, and demethylthioation of LIN. Hydroxylamine, an intermediate of ammonia oxidation, chemically reacted with LIN and the selected FQs, with removals exceeding 90%. Collectively, these findings provide important fundamental insights into the roles of different ammonia oxidizers and their intermediates on LIN and FQ biotransformation in nitrifying environments including wastewater treatment systems.

show abstract

Section: Transformation Product Identification Of Lin and Fqsmentioning

confidence: 99%

Biotransformation of lincomycin and fluoroquinolone antibiotics by the ammonia oxidizers AOA, AOB and comammox: A comparison of removal, pathways, and mechanisms

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Traditional composting is an extensively practiced, effective, and safe strategic way of recycling LM as it inactivates pathogens, detoxifies micropollutants, and stabilizes organics and nutrients by converting them into the stable humic substances. − The dominant phyla such as Actinobacteria , Bacteroidetes , Firmicutes , and Proteobacteria occupy over 90.0% of the bacterial community during LM composting, accelerating organic matter decomposition and humification (also listed in Table S1). − These processes ameliorate the fertilizer quality of manure because it releases nutrients more slowly in a balanced manner, while uncomposted fresh manure contains large amounts of health-damaging contaminants and pathogens that can impair plants and pollute eco-environments .…”

Section: Introductionmentioning

confidence: 99%

Insights into the Applications of Extracellular Laccase-Aided Humification in Livestock Manure Composting

Sun

Latif

et al. 2022

Environ. Sci. Technol.

View full text Add to dashboard Cite

Traditional composting is a well-suited biotechnology for onfarm management of livestock manure (LM) but still leads to the release of toxic micropollutants and imbalance of nutrients. One in situ exoenzymeassisted composting has shown promise to ameliorate the agronomical quality of end products by improving humification and polymerization. The naturally occurring extracellular laccase from microorganisms belongs to a multicopper phenoloxidase, which is verified for its versatility to tackle micropollutants and conserve organics through the reactive radical-enabled decomposition and polymerization channels. Laccase possesses an indispensable relationship with humus formation during LM composting, but its potential applications for the harmless disposal and resource utilization of LM have until now been overlooked. Herein, we review the extracellular laccase-aided humification mechanism and its optimizing strategy to maintain enzyme activity and in situ production, highlighting the critical roles of laccase in treating micropollutants and preserving organics during LM composting. Particularly, the functional effects of the formed humification products by laccaseamended composting on plant growth are also discussed. Finally, the future perspectives and outstanding questions are summarized. This critical review provides fundamental insights into laccase-boosted humification that ameliorates the quality of end products in LM composting, which is beneficial to guide and advance the practical applications of exoenzyme in humification remediation, the carbon cycle, and agriculture protection.

show abstract

“…LIN is not fully metabolized by animals and the majority is excreted through urine and feces, and therefore pass into the environment. Moreover, LIN is stable in the aquatic system, and has a half-life time up to 15 days and even 2033 days in certain conditions [3,4]. In line with this, a substantial amount of LIN has been measured in surface waters within the level of 175 to 1413 ng L −1 and was deemed a priority compound based on its ecological risk to algae [5][6][7].…”

Section: Introductionmentioning

confidence: 83%

Lincomycin-Induced Transcriptional Alterations in the Green Alga Raphidocelis subcapitata

et al. 2020

View full text Add to dashboard Cite

Lincomycin (LIN), as a waterborne contaminant, may pose a threat to algal health and may affect the provision of ecosystem services. In addition, the molecular mechanisms of lincomycin in algae are still unknown. Here, we attempted to use the transcriptome analysis to elucidate for the first time the potential impact of LIN at an environmentally relevant concentration on the algal growth, and verify the hypothesis that lincomycin can disrupt algal protein synthesis by combining with its subunits of ribosome at high-LIN level. In this study, 7-day growth inhibition tests and RNA-seq sequencing were conducted in Raphidocelis subcapitata (R. subcapitata) in response to a LIN at the concentrations of 0.5 µg L−1 (low), 5 µg L−1 (medium), and 400 µg L−1 (high) treatment groups. A negligible influence on algal growth and merely 21 (21 up- and 0 downregulated) differentially expressed genes (DEGs) was observed at low concentration of LIN, and medium groups showed a 13.4% inhibition and 92 (64 up- and 48 downregulated) DEGs, while high-LIN dosing caused 65.4% reduction in algal growth and 2514 (663 up- and 1851 downregulated) DEGs. In 0.5 and 5 µg L−1 groups, LIN upregulated the genes in the process of photosynthesis consisting of photosynthesis-antenna proteins, and porphyrin and chlorophyll metabolism pathways, suggesting that photosynthesis at low LIN exposure was more sensitive than algal growth. Whereas DEGs in the 400 µg L−1 group were mostly enriched in carbohydrate, carbon fixation in photosynthetic organisms, and nucleotide metabolism pathways. Furthermore, genes involved in detoxification processes were nearly downregulated in high-LIN group. In addition, genes encoding the antioxidant enzymes in the peroxisome pathway such as superoxide dismutase (sod2), peroxin-2 (pex2), 2,4-dienoyl-CoA reductase ((3E)-enoyl-CoA-producing) (decr2) were upregulated, which are responsible for deleting extra intracellular reactive oxygen species (ROS) caused by LIN to protect algal health, suggesting the occurrence of oxidative stress. Taken together, this is the first meticulous study unraveling the molecular mechanism of antibiotics in algae.

show abstract

Effects of co-composting of lincomycin mycelia dregs with furfural slag on lincomycin degradation, degradation products, antibiotic resistance genes and bacterial community

Cited by 43 publications

References 44 publications

Biotransformation of lincomycin and fluoroquinolone antibiotics by the ammonia oxidizers AOA, AOB and comammox: A comparison of removal, pathways, and mechanisms

Biotransformation of lincomycin and fluoroquinolone antibiotics by the ammonia oxidizers AOA, AOB and comammox: A comparison of removal, pathways, and mechanisms

Insights into the Applications of Extracellular Laccase-Aided Humification in Livestock Manure Composting

Lincomycin-Induced Transcriptional Alterations in the Green Alga Raphidocelis subcapitata

Contact Info

Product

Resources

About