Effects of clay's chemical interactions on biocementation

Cardoso, Rafaela; Pires, Inês; Duarte, Sofia O. D.; Monteiro, Gabriel A.

doi:10.1016/j.clay.2018.01.035

Cited by 93 publications

(54 citation statements)

References 36 publications

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“…Cheng and Shahin [25] used clayey sand with up to 20% clay content and assessed three MICP treatment methods including injection, premixing, and diffusion. Cardoso et al [26] carried out oedometer and Brazilian splitting tests to characterize the biocementation effect on clayey sand and highlighted the importance of chemical effects originated from the clay fraction on soil behavior. Li et al [27] blended fly ash at different concentrations into the MICP-treated expansive soil and showed that biocement and fly ash contributed jointly to the soil improvement.…”

Section: Introductionmentioning

confidence: 99%

Desiccation Cracking Behavior of MICP-Treated Bentonite

et al. 2019

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This study aims to characterize the effect of microbial-induced calcite precipitation (MICP) on the desiccation cracking behaviors of compacted calcium bentonite soils. We prepare six groups of samples by mixing bentonites with deionized water, pure bacteria solution, pure cementation solution, and mixed bacteria and cementation solutions at three different percentages. We use an image processing tool to characterize the soil desiccation cracking patterns. Experimental results reveal the influences of fluid type and mixture percentage on the crack evolution and volumetric deformation of bentonite soils. MICP reactions effectively delay the crack initiation and remediate desiccation cracking, as reflected by the decreased geometrical descriptors of the crack pattern such as surface crack ratio. The mixture containing 50% bacteria and 50% cementation solutions maximizes the MICP treatment and works most effectively in lowering the soil cracking potential. This study provides new insights into the desiccation cracking of expansive clayey soils and shows the potential of MICP applications in the crack remediation.

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Section: Introductionmentioning

confidence: 99%

Desiccation Cracking Behavior of MICP-Treated Bentonite

et al. 2019

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“…Strain P6 (Corynebacterium urealyticum), belonging to the first group of calcifying strains ( Figure 5), was selected for deep sand biocementation base on its capability to precipitate CaCO3 crystals in liquid B4 medium, to hydrolyze urea and to grow in anaerobiosis (Tables 3 and 5 After the biotreatment, the sand samples did not reach a cementation level sufficient to be subjected to the shear strength test, even in the presence of urea, calcium chloride and low clay fraction [38,39].…”

Section: P1 P2 P3 P4 M5 P6 P7 P8 P9 P10 P11mentioning

confidence: 99%

“…The various ecological implications of BCP in biotechnology have been described by Zhu and Dittrich [37]. BCP applications include: bioremediation of metal contaminated soil and ground water; restoration and preservation of calcareous sculptural artefacts, historical monuments and civil buildings; bioconcrete; different geotechnical-engineering applications such as soil/sand strength, sand impermeability, mitigation of liquefaction, soil erosion control, impermeabilization of polluted soils and other soil improvement projects; Microbial Enhanced Oil Recovery (EOR); CO2 sequestration; filler for rubber, plastic and ink [38][39][40][41].…”

Section: Introductionmentioning

confidence: 99%

A Novel Approach to Isolation and Screening of Calcifying Bacteria for Biotechnological Applications

Cacchio¹,

Gallo²

2019

Preprint

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Bacterial Calcium-carbonate Precipitation (BCP) has been studied for multiple applications such as remediation, consolidation and cementation. Isolation and screening of strong calcifying bacteria is the main task of BCP-technique. In this paper we studied CaCO3 precipitation by different bacteria isolated from a rhizospheric soil in both solid and liquid media. It has been found, through culture-depending studies, that bacteria belonging to Actinobacteria, Gammaproteobacteria and Alphaproteobacteria are the dominant bacteria involved in CaCO3 precipitation in this environment. Pure and mixed cultures of selected strains were applied for sand biocementation experiments. Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray Spectroscopy (EDS) analyses of the biotreated samples revealed the biological nature of the cementation and the effectiveness of the biodeposition treatment by mixed cultures. X-ray diffraction (XRD) analysis confirmed that all the calcifying strains selected for sand biocementation precipitated CaCO3, mostly in the form of calcite. In this study Biolog® Eco-plate is evaluated as a useful method for a more targeted choice of the sampling site with the purpose of obtaining interesting candidates for BCP applications. Furthermore, ImageJ software was investigated, for the first time to our knowledge, as a potential method to screen high CaCO3 producer strains.

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“…According to Neupane [50] and Cardoso et al [11], coarse-grained soils are good candidates for an effective MICP technique; however, this study was conducted with the aim of understanding the efficacy of MICP technique in low plasticity soils in order to establish the relationship between plasticity characteristics and MICP with different compositional variables (i.e., S. pasteurii suspension density and S. pasteurii suspension-cementation reagent mix ratio). The optimal requirements for different organisms are different in terms of bacterial densities, water requirements and other compositional variables for effective MICP output [48].…”

Section: Introductionmentioning

confidence: 99%

Plasticity characteristics of lateritic soil treated with Sporosarcina pasteurii in microbial-induced calcite precipitation application

et al. 2019

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The plasticity characteristics of lateritic soil with varying Sporosarcina pasteurii (S. pasteurii) suspension density and compositional variables were evaluated in microbial-induced calcite precipitation (MICP) application. The liquid limit value of the natural lateritic soil was used to prepare samples with three mix proportions of the bacteria and cementation reagent (i.e., 25% bacteria-75% cementation reagent, 50% bacteria-50% cementation reagent and 75% bacteria-25% cementation reagent). The S. pasteurii suspension densities used to trigger the MICP process are 0, 0.5, 2.0, 4.0, 6.0 and 8.0 McFarland standards (i.e., 0, 1.50 × 10 8 , 6.0 × 10 8 , 1.20 × 10 9 , 1.80 × 10 9 and 2.40 × 10 9 cells/ml, respectively). Tests carried out on the treated specimens include Atterberg limits and linear shrinkage as well as calcite content using the acid wash method. Results obtained showed a general decrease in the Atterberg limit values with higher S. pasteurii suspension density. The best improvement of plasticity index was achieved for lateritic soil prepared with 75% S. pasteurii and 25% cementation reagent at S. pasteurii suspension density of 2.40 × 10 9 cells/ml with a corresponding peak 6.0% calcite content. Also, a maximum 4% contaminant concentration of a synthetic leachate produced the best S. pasteurii growth pattern.

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Effects of clay's chemical interactions on biocementation

Cited by 93 publications

References 36 publications

Desiccation Cracking Behavior of MICP-Treated Bentonite

Desiccation Cracking Behavior of MICP-Treated Bentonite

A Novel Approach to Isolation and Screening of Calcifying Bacteria for Biotechnological Applications

Plasticity characteristics of lateritic soil treated with Sporosarcina pasteurii in microbial-induced calcite precipitation application

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