Effects of cisplatin and γ-irradiation on cell survival, the induction of chromosomal aberrations and apoptosis in SW-1573 cells

Bergs, Judith; Franken, Nicolaas A.P.; Cate, Rosemarie ten; Bree, Chris van; Haveman, J.

doi:10.1016/j.mrfmmm.2005.08.002

Cited by 32 publications

(34 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…by Bergs et al (2006). Wilkins et al (1993), who investigated the effect of cisplatin and radiation on repair of PLD in confluent cultures of two different brain tumour cell lines, also reported a cisplatin-induced radiation sensitization due to inhibition of PLD repair.…”

Section: Discussionmentioning

confidence: 99%

“…As reviewed by Bergs et al (2007), it has been demonstrated by several studies that all three agents applied together yield a clear enhancement of effects compared to single-or bi-modality treatments. Hyperthermia increases radiation sensitivity by inhibiting homologous recombination repair and in addition cisplatin increases the radiation sensitivity by disrupting endjoining repair and base excision repair (Bergs et al 2006(Bergs et al , 2013Oei et al 2015). This explains the increased effect for the tri-modality strategy.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of enhancement at small and large radiation doses for effectiveness of inactivation in cultured cells by combining two agents with radiation

Franken

Kok

Crezee

et al. 2016

International Journal of Radiation Biology

View full text Add to dashboard Cite

Purpose: To evaluate the enhancement effect of two combined radiation-sensitizing agents in mammalian cells at small doses as compared to large doses using the linear-quadratic (LQ) mathematical model. Methods and materials: Data on clonogenic assays concerning the radio-enhancement effects of combined halogenated pyrimidines and hyperthermia or combined cisplatin and hyperthermia, as published in earlier reports, were analyzed according to the LQ-formula:). Effects of sensitizing agents on the linear parameter a and the quadratic parameter b are compared in order to evaluate differences depending on the applied dose, the possible relations to mechanisms of radiation sensitization and to derive suggestions for applications. Results: The values of the linear parameter a, which determines the effectiveness at low doses, are for all cell lines and all conditions more increased than the values of the parameter b which has a higher contribution at larger radiation doses. The combination of hyperthermia with halogenated pyrimidines to radiation as well as the combination of hyperthermia and cisplatin to radiation significantly increases the value of the linear parameter a, as compared to radiation alone or radiation combined with a single agent. Conclusions:The radiation enhancement factors of the values of linear and quadratic parameters demonstrate that the sensitizing agents have a larger effect on the linear parameter which is dominant at low radiation doses as is used in fractionated-radiation treatment in the clinic. Moreover, the effect is even further increased when two radiation sensitizers are used. ARTICLE HISTORY

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Analysis of enhancement at small and large radiation doses for effectiveness of inactivation in cultured cells by combining two agents with radiation

Franken

Kok

Crezee

et al. 2016

International Journal of Radiation Biology

View full text Add to dashboard Cite

show abstract

“…Cisplatin-based chemoradiotherapy has become standard treatment for, among others, locally advanced cervical carcinoma (30) and locally advanced non-small-cell lung cancer (NSCLC) (31). There have been numerous studies on the radiation-sensitising effect of cisplatin; however, the results vary from a clear cisplatin-induced radiosensitisation (24,25,(32)(33)(34) to a merely additive effect on cell survival (35). Cisplatin and radiation share a common cellular target, DNA (36).…”

Section: Cisplatinmentioning

confidence: 99%

Cell survival and radiosensitisation: Modulation of the linear and quadratic parameters of the LQ model

Franken

Oei

Kok

et al. 2013

International Journal of Oncology

View full text Add to dashboard Cite

Abstract.The linear-quadratic model (LQ model) provides a biologically plausible and experimentally established method to quantitatively describe the dose-response to irradiation in terms of clonogenic survival. In the basic LQ formula, the clonogenic surviving fraction S d ̸S 0 following a radiation dose d (Gy) is described by an inverse exponential approximation:, wherein α and β are experimentally derived parameters for the linear and quadratic terms, respectively. Radiation is often combined with other agents to achieve radiosensitisation. In this study, we reviewed radiation enhancement ratios of hyperthermia (HT), halogenated pyrimidines (HPs), various cytostatic drugs and poly(ADP-ribose) polymerase-1 (PARP1) inhibitors expressed in the parameters α and β derived from cell survival curves of various mammalian cell cultures. A significant change in the α/β ratio is of direct clinical interest for the selection of optimal fractionation schedules in radiation oncology, influencing the dose per fraction, dose fractionation and dose rate in combined treatments. The α/β ratio may increase by a mutually independent increase of α or decrease of β. The results demonstrated that the different agents increased the values of both α and β. However, depending on culture conditions, both parameters can also be separately influenced. Moreover, it appeared that radiosensitisation was more effective in radioresistant cell lines than in radiosensitive cell lines. Furthermore, radiosensitisation is also dependent on the cell cycle stage, such as the plateau or exponentially growing phase, as well as on post-treatment plating conditions. The LQ model provides a useful tool in the quantification of the effects of radiosensitising agents. These insights will help optimize fractionation schedules in multimodality treatments.

show abstract

“…An additional advantage of the LQ model is that its parameters can be discussed in terms of specific mechanisms of cell inactivation by radiation (Barendsen, 1990(Barendsen, , 1994. Linear-quadratic analyses of hyperthermia-or chemotherapy-induced radiation sensitisation have been reported for exponentially growing and plateau phase human tumour cells in culture and for different experimental rodent cell lines (Franken et al, 1997a(Franken et al, , 1997bvan Bree et al, 1997van Bree et al, , 2000Castro Kreder et al, 2004;Bergs et al, 2006. When the additional treatment results in increases of the value of the -parameter, this indicates that this treatment radiosensitizes at clinically relevant doses.…”

mentioning

confidence: 99%

Radiosensitization with Hyperthermia and Chemotherapeutic Agents: Effects on Linear-Quadratic Parameters of Radiation Cell Survival Curves

Franken¹,

Hovingh²,

Oei³

et al. 2012

Current Topics in Ionizing Radiation Research

View full text Add to dashboard Cite

Effects of cisplatin and γ-irradiation on cell survival, the induction of chromosomal aberrations and apoptosis in SW-1573 cells

Cited by 32 publications

References 30 publications

Analysis of enhancement at small and large radiation doses for effectiveness of inactivation in cultured cells by combining two agents with radiation

Analysis of enhancement at small and large radiation doses for effectiveness of inactivation in cultured cells by combining two agents with radiation

Cell survival and radiosensitisation: Modulation of the linear and quadratic parameters of the LQ model

Radiosensitization with Hyperthermia and Chemotherapeutic Agents: Effects on Linear-Quadratic Parameters of Radiation Cell Survival Curves

Contact Info

Product

Resources

About