Effects of cisplatin-5-fluorouracil combination therapy on oxidative stress, DNA damage, mitochondrial apoptosis, and death receptor signalling in retinal pigment epithelium cells

Güçlü, Hande; Doğanlar, Zeynep Banu; Gürlü, Vuslat; Özal, Altan; Doğan, Ayten; Turhan, Meryem Ayşenur; Doğanlar, Oğuzhan

doi:10.1080/15569527.2018.1456548

Cited by 22 publications

(7 citation statements)

References 59 publications

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“…In addition, cisplatin is a primary treatment drug in many solid cancers, and its anticancer effects are induced by the generation of DNA damage and mitochondrial apoptosis [ 124 ]. However, the efficacy of treatment with cisplatin is restricted by the development of chemoresistance [ 125 ].…”

Section: Autophagy As Drug-resistant Factor Of Tumorsmentioning

confidence: 99%

The Roles of Autophagy in Cancer

Yun

Lee

2018

IJMS

742

557

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Autophagy is an intracellular degradative process that occurs under several stressful conditions, including organelle damage, the presence of abnormal proteins, and nutrient deprivation. The mechanism of autophagy initiates the formation of autophagosomes that capture degraded components and then fuse with lysosomes to recycle these components. The modulation of autophagy plays dual roles in tumor suppression and promotion in many cancers. In addition, autophagy regulates the properties of cancer stem-cells by contributing to the maintenance of stemness, the induction of recurrence, and the development of resistance to anticancer reagents. Although some autophagy modulators, such as rapamycin and chloroquine, are used to regulate autophagy in anticancer therapy, since this process also plays roles in both tumor suppression and promotion, the precise mechanism of autophagy in cancer requires further study. In this review, we will summarize the mechanism of autophagy under stressful conditions and its roles in tumor suppression and promotion in cancer and in cancer stem-cells. Furthermore, we discuss how autophagy is a promising potential therapeutic target in cancer treatment.

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Section: Autophagy As Drug-resistant Factor Of Tumorsmentioning

confidence: 99%

The Roles of Autophagy in Cancer

Yun

Lee

2018

IJMS

742

557

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“…The observed effect of deterioration after the regimen could be attributed to many factors, such as ROS overproduction associated with some of the mechanisms of actions of the applied chemotherapeutic treatment and the subsequent exhaustion of antioxidant pools in the recruited patients. Such effects have often been observed by many research groups during cancer treatment [39,40]. The elevation in the MDA products, indicating intensification of lipid peroxidation and increased sensitivity to oxidative molecular damage, is another evidence for the decrease in radical-trapping blood plasma capacity.…”

Section: Discussionmentioning

confidence: 83%

Dihydropyrimidine dehydrogenase level and the redox status in patients with colorectal cancer are prognostic for adverse effects of fluoropyrimidines

Hristova-Avakumova

Minchev

Kamenova

et al. 2021

Biotechnology & Biotechnological Equipment

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Drug resistance and toxicity are the most widespread limitations in the pursuit of sufficient antitumor effectiveness and successful control of oncological diseases, including colorectal cancer (CRC). Herein the objective was to investigate some hematological side effects of the chemotherapeutic regimen FOLFOX-4, their relation to dihydropyrimidine dehydrogenase (DPD) levels and the associated alterations in CRC patients' plasma free-radical scavenging properties and extent of oxidative molecular damage. Thirty-eight patients with histologically confirmed CRC diagnoses, assigned to chemotherapy with the FOLFOX-4 regimen, were recruited. The diagnostic methods included a complete physical examination, blood routine test and general biochemistry. The DPD levels were assayed. The patients' plasma free-radical scavenging properties and extent of molecular oxidative damage were determined by spectrophotometry and enhanced chemiluminescence. The clinico-pathological and demographics characteristics of the patients were in agreement with the reports from retrospective cohort studies. The FOLFOX-4 regimen induced a decrease in plasma free-radical scavenging properties and increased extent of lipid peroxidation. White blood cells, granulocytes and lymphocytes decreased significantly after the first cycle of the therapy. The patients' DPD level decreased statistically significantly in the case of severe reduction (more than 25%) of white blood cells and granulocyte counts. The obtained data are in agreement with already known facts concerning the side effects of the FOLFOX-4 regimen and associated changes in redox homeostasis. Genetic predisposition to effectively metabolize and tolerate the applied therapy, i.e. DPD levels, could modulate some aspects of the observed changes in the aforementioned parameters.

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“…cDNA synthesis was realized with the thermal cycler Applied Biosystems® Veriti® (Thermo Scienti c, USA) at the three steps which were Step 1: 25 °C, 10 min; Step 2: 37 °C, 120 min; Step 3: 85 °C, 5 min, respectively. The synthesized cDNA was kept at -20 °C for later analysis steps [25,28,[30][31][32][33]. Expression levels of the mitochondrial apoptosis genes in both cancer cells of vehicle treated control and IC 50 doses treated with BBHMP or Pt(II) complex for 24 hours were determined with quantitative real-time-polymerase chain reaction (qRT-PCR) by using the PowerSYBR® Select Master Mix (Life Technologies, USA) by an ABI 7500 Real-Time PCR system (Thermo Scienti c, USA).…”

Section: Gene Expression Analysismentioning

confidence: 99%

Synthesis and Biological Activity of a New Pt(II) Complex Involving 4-bromo-2,6-bis-hydroxymethyl-phenol and Nicotinamide

Altun

Yoruç

Boz

2021

Preprint

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In the presented study, Pt(II) complex including 4-Bromo-2,6-bis-hydroxymethyl-phenol (BBHMP) and nicotinamide (NA) was synthesized and structurally analyzed by using spectral and thermal analysis methods. The BBHMP and its Pt(II) complex involving BBHMP and NA were investigated for their antimicrobial, cytotoxicity, gen expression and antioxidant properties. The antimicrobial activity results showed that the platinum complex displayed moderate antibacterial and antifungal activities. The cytotoxicity of the BBHMP and platinum complex were determined against human prostate (DU145) and breast (MCF7) cancer cell lines by applying MTT assay. Cytotoxicity results suggest that the Pt(II) complex exhibited moderate cytotoxicity against growth of used cancer cell lines when compared with the reference drug cisplatin. Gen expression results proved that Pt(II) complex is a special bioactive chemical constituent and potential anticancer agent. In addition, the complex demonstrated important antioxidant activity.

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Effects of cisplatin-5-fluorouracil combination therapy on oxidative stress, DNA damage, mitochondrial apoptosis, and death receptor signalling in retinal pigment epithelium cells

Cited by 22 publications

References 59 publications

The Roles of Autophagy in Cancer

The Roles of Autophagy in Cancer

Dihydropyrimidine dehydrogenase level and the redox status in patients with colorectal cancer are prognostic for adverse effects of fluoropyrimidines

Synthesis and Biological Activity of a New Pt(II) Complex Involving 4-bromo-2,6-bis-hydroxymethyl-phenol and Nicotinamide

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