Effects of cimetidine and its metal complexes on nitroblue tetrazolium and ferricytochrome c reduction by superoxide radicals

Kirkova, Margarita; Atanassova, Mila; Russanov, E

doi:10.1016/s0306-3623(99)00015-4

Cited by 18 publications

(4 citation statements)

References 33 publications

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“…The superoxide anion scavenging activity of 2-APB and its analogues was determined using the nitro-blue tetrazolium (NBT; Sigma-Aldrich, Saint Louis, MO, USA) reduction method as previously described with some modifications [ 21 ]. The superoxide anion was produced by KO 2 .…”

Section: Methodsmentioning

confidence: 99%

Two Benzene Rings with a Boron Atom Comprise the Core Structure of 2-APB Responsible for the Anti-Oxidative and Protective Effect on the Ischemia/Reperfusion-Induced Rat Heart Injury

Shen

Lee

et al. 2021

Antioxidants

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To identify the core structure of 2-aminoethoxydiphenyl borate (2-APB) responsible for the anti-oxidative and protective effect on the ischemia/reperfusion (I/R)-induced heart injury, various 2-APB analogues were analyzed, and several antioxidant assays were performed. Cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Myocardial infarct size was quantified using triphenyl tetrazolium chloride (TTC) staining. The levels of tumor necrosis factor-alpha (TNF-α) and cleaved-caspase-3 protein were evaluated as an indicator for the anti-inflammatory and anti-apoptotic effect, respectively. Our data show that 2-APB, diphenylborinic anhydride (DPBA) and 3-(diphenylphosphino)-1-propylamine (DP3A) all exerted the anti-oxidative activity, but only 2-APB and DPBA can scavenge H2O2. 2-APB and DPBA can potently inhibit hydrogen peroxide (H2O2)- and hypoxanthine/xanthine oxidase (HX/XOD)-induced increases in intracellular H2O2 and H9c2 cell death. 2-APB and DPBA were able to decrease the I/R-induced adult rat cardiomyocytes death, myocardial infarct size, and the levels of malondialdehyde (MDA) and creatine kinase-MB (CK-MB). Our results suggest that the two benzene rings with a boron atom comprise the core structure of 2-APB responsible for the anti-oxidative effect mediated through the reaction with H2O2 and generation of phenolic compounds, which in turn reduced the I/R-induced oxidative stress and injury in the rat heart.

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Section: Methodsmentioning

confidence: 99%

Two Benzene Rings with a Boron Atom Comprise the Core Structure of 2-APB Responsible for the Anti-Oxidative and Protective Effect on the Ischemia/Reperfusion-Induced Rat Heart Injury

Shen

Lee

et al. 2021

Antioxidants

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“…Finally, erythrocyte samples were analyzed for erythrocyte glutathione concentrations as described by Paglia and Valentine (1967) and Flohe and Gunzler (1984). The analyses of catalase concentrations (Aebi, 1984) and superoxide dismutase (SOD) activities (Kirkova et al, 1999) were performed as described before.…”

Section: Ldl-receptor Mrnamentioning

confidence: 99%

Effects of plant sterol and stanol ester consumption on lipid metabolism, antioxidant status and markers of oxidative stress, endothelial function and low-grade inflammation in patients on current statin treatment

et al. 2007

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Objective: The present study was designed to examine for the first time, side-by-side, the effects of plant sterol and stanol consumption on lipid metabolism and markers of antioxidant status, oxidative stress, endothelial dysfunction and low-grade inflammation in subjects on stable statin-treatment. Design: Double-blind, randomized, placebo-controlled, intervention trial. Setting: University. Subjects: Forty-five patients on current statin treatment were recruited via newspaper advertisements. Data of 41 patients were used in statistical analysis. Intervention: Subjects consumed margarine with no added plant sterols or stanols for 4 weeks and were then divided into three groups of 15 subjects. For the next 16 weeks, one group continued with the control margarine and the other two groups with either a plant sterol-or stanol (2.5 g/day)-enriched margarine. Blood was sampled at the end of the run-in and intervention periods. Results: Plant sterol and stanol consumption significantly (P ¼ 0.026) reduced low-density lipoprotein (LDL) cholesterol by 0.34 mmol/l (95% confidence interval (CI), À0.67 to À0.04 mmol/l). No effects were shown on enzymatic and non-enzymatic antioxidants and markers of oxidative modification of lipids and DNA. In addition, no effect was found on soluble adhesion molecules, C-reactive protein and monocyte chemotactic protein-1 concentrations. Conclusions: We conclude that 16 weeks of plant sterol or stanol consumption did not affect markers of antioxidant status, oxidative stress, endothelial dysfunction and low-grade inflammation in patients on stable statin treatment, despite a significant reduction of LDL cholesterol.

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“…Cimetidine ( cim ) is one of the most potent histamine H 2 ‐receptor antagonists for inhibiting excessive acid secretion caused by histamine and is currently used for treating peptic ulcers. It has been hypothesized that both its therapeutic effects, namely the anti‐inflammatory and anti‐ulcer properties, and the favorable evolution in rheumatoid arthritis as well as some of the side effects, such as male sexual dysfunctions frequently reported during treatment with cim , are mediated by its interaction with Cu(II) and Zn(II) metal ions 1–4 . Cim has a satisfactory superoxide dismutase (SOD) like activity too, so it may provide a possible therapeutic approach against prion diseases 5.…”

Section: Introductionmentioning

confidence: 99%

Raman and SERS study on cimetidine–metal complexes with biomedical interest

Bonora

Foggia

Tugnoli

et al. 2010

J Raman Spectroscopy

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Cimetidine (cim) is one of the most potent histamine H 2 -receptor antagonists for inhibiting excessive acid secretion caused by histamine; it has been hypothesized that the therapeutic effects can be related to its interactions with metal ions. Raman spectra of the solid cim with Co(II), Cu(II), Ni(II) and Zn(II) metal complexes show that they can adopt two different structures: one is octahedral and the other, with Zn(II), is probably tetrahedral. The octahedral structure appears to be distorted both by the different metal ions as well as by the different anion present. The study was extended to very dilute solutions (ppm range) by using the surface-enhanced Raman scattering (SERS) technique, mimicking the physiological concentrations of cim and its metal complexes. SERS spectra suggest that, upon the binding of cim to silver colloids, the formation of stable 1 : 2 cim-metal complexes is excluded, the formation of 1 : 1 adduct appearing more probable; in this product the metal reaches its total coordination shell by complexion with water molecules. To better explain the binding mechanism of cim to a metal (Ag) surface, we performed theoretical B3LYP calculations on cim alone as well as on cim bonded to an Ag 2 metal cluster in presence of water, observing a sufficiently good agreement between experimental and theoretical wavenumbers.

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Effects of cimetidine and its metal complexes on nitroblue tetrazolium and ferricytochrome c reduction by superoxide radicals

Cited by 18 publications

References 33 publications

Two Benzene Rings with a Boron Atom Comprise the Core Structure of 2-APB Responsible for the Anti-Oxidative and Protective Effect on the Ischemia/Reperfusion-Induced Rat Heart Injury

Two Benzene Rings with a Boron Atom Comprise the Core Structure of 2-APB Responsible for the Anti-Oxidative and Protective Effect on the Ischemia/Reperfusion-Induced Rat Heart Injury

Effects of plant sterol and stanol ester consumption on lipid metabolism, antioxidant status and markers of oxidative stress, endothelial function and low-grade inflammation in patients on current statin treatment

Raman and SERS study on cimetidine–metal complexes with biomedical interest

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