Effects of chronic sublethal exposure to waterborne Cu, Cd or Zn in rainbow trout 2: tissue specific metal accumulation

McGeer, James C.; Szebedinszky, Cheryl; McDonald, D. G.; Wood, Chris M.

doi:10.1016/s0166-445x(99)00106-x

Cited by 156 publications

(68 citation statements)

References 22 publications

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“…Moreover, Cu concentrations in livers of trout exposed to the higher concentration (80 g/l) were similar to levels reported by Laflamme et al (2000) in perch from field studies in the mining area of Abitibi, Qc. Similar to other laboratory and field studies, liver was the principal organ of Cu accumulation, followed by other tissues (McGeer et al, 2000b;Audet and Couture, 2003;Lévesque et al, 2003;Giguere et al, 2004).…”

Section: Discussionsupporting

confidence: 83%

Effects of Cu on plasma cortisol and cortisol secretion by adrenocortical cells of rainbow trout (Oncorhynchus mykiss)

2006

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Fish are exposed to multiple stressors, often acting concurrently, in their environment. To evaluate the potential of Cu to act as a chemical stressor, rainbow trout (Oncorhynchus mykiss) were exposed to Cu (30 or 80 g/l) for 30 days in the laboratory and they were subjected to a physical stressor (1 min air exposure) before sampling. Physiological stress indicators in the whole fish as well as cortisol secretion by adrenocortical cells in vitro were measured. Fish exposed to Cu had a lower condition factor, hepatosomatic index, plasma glucose, hepatic glycogen and gill Na + /K + -ATPase activity compared to controls. Exposure to Cu did not have an effect on basal plasma cortisol (fish sampled without air exposure stress) however, the air exposure-induced increase in plasma cortisol was lower in fish exposed to Cu. Cortisol secretion stimulated by ACTH in vitro was greater in adrenocortical cells isolated from fish exposed to Cu in vivo but in vitro exposure to Cu consistently impaired cortisol secretion. Our results indicate that Cu at high concentrations disrupts cortisol secretion through a direct toxic effect on adrenocortical cells while low concentrations resulting from a 30-day exposure to environmentally relevant Cu concentrations enhances cortisol secretion in response to ACTH in vitro.

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Section: Discussionsupporting

confidence: 83%

Effects of Cu on plasma cortisol and cortisol secretion by adrenocortical cells of rainbow trout (Oncorhynchus mykiss)

2006

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“…However, Cu accumulation in the gills of olive flounder was lower than in the liver and intestines. Similar results have also been observed in rainbow trout (Miller et al, 1993;McGeer et al, 2000b), tilapia (Pelgrom et al, 1995), Senegales sole (Arellano et al, 1999), and sea bream (Wong et al, 1999). Mazon and Femandes (1999) suggested that these results were attributable to continuous Cu regulation, during which the gills absorb Cu and subsequently transfer it into the bloodstream.…”

Section: Discussionsupporting

confidence: 71%

“…Generally, the kidneys play a major role in the detoxification and excretion of toxins via the induction of metal-binding proteins such as MT (Roesijadi and Robinson, 1994), similar to the liver. Therefore, tissues such as the kidneys, which are major producers of MT, show high accumulation of Cu in fish (Pelgrom et al, 1995;Mazon and Femandes, 1999;McGeer et al, 2000b). However, Cu accumulation in the kidney of flounder was about an order of magnitude lower than in the intestine and liver.…”

Section: Discussionmentioning

confidence: 99%

“…Cu accumulation in different tissues varies depending on the source of uptake and whether Cu is waterborne or ingested as food. Whatever the exposure method, Cu accumulates substantially in gills, intestines, and mainly in the liver and kidneys (Giles, 1988;Mazon and Femandes, 1999;McGeer et al, 2000b). Fish are an important human food resource as well as crucial components of aquatic ecosystems; thus, assessing the effects of Cu on fish is vital from both an anthropogenic and ecological perspective.…”

Section: Introductionmentioning

confidence: 99%

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Cu Accumulation and Elimination in the Tissues of the Olive Flounder Paralichthys olivaceus

Kim¹,

Jang²,

Lee³

et al. 2011

Fisheries and aquatic sciences

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Olive flounder, Paralichthys olivaceus were exposed to waterborne Cu (control, 50, 80, 150, and 320 µg/L) for 30 days and then depurated for 20 days to investigate the effects of waterborne Cu exposure on growth, accumulation, and elimination. The weightspecific growth rate was significantly negatively related to waterborne Cu concentrations at 150 and 320 µg/L. The order of Cu accumulation in different tissues of exposed fish was liver>intestines>gills>kidneys>muscle, suggesting that the liver is more important than other tissues for the storage of Cu in olive flounder. The accumulation factor for the gills, intestines, liver, and muscle increased with increasing exposure time, and accumulation was negatively related to exposure concentration for the gills, kidneys, and muscle. Cu concentrations in the gills, intestines, and liver continuously decreased for 20 days of depuration. The fastest elimination rate occurred in the intestines at all exposure concentrations, and the order of Cu elimination in the different tissues was intestines>liver>gills.

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“…Organ specificity in metal accumulation may also be related to the metal speciation and the regulation of essential and non-essential metals (Hanson 1997, Baskin 2000, McGeer et al 2000, Burgos & Rainbow 2001. Although most studies have quantified the metal concentrations in fish collected from the field, where they are exposed to metals both in the aqueous and in the dietary phases, it is difficult to quantitatively distinguish the metal uptake via food or water.…”

Section: Resale or Republication Not Permitted Without Written Consenmentioning

confidence: 99%

Exposure and potential food chain transfer factor of Cd, Se and Zn in marine fish Lutjanus argentimaculatus

Xu¹,

Wang

2002

Mar. Ecol. Prog. Ser.

123

115

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Radiotracer techniques were employed to quantify the rates of uptake from aqueous and dietary sources, and rates of elimination of Cd, Se and Zn by a marine predatory fish, the mangrove snapper Lutjanus argentimaculatus. The relative significance of bioaccumulation of metals by fish from water and food and the food chain transfer factor were then assessed using a kinetic modeling approach. Cd and Zn in the aqueous phase exhibited an approximately linear uptake pattern over a 1 to 2 d exposure, whereas Se exhibited a 2-compartmental uptake at a low ambient concentration, with a slow initial uptake followed by a rapid increase in Se influx. Most of the accumulated aqueous Se and Zn were incorporated into the muscles, whereas Cd was evenly distributed in the viscera and the remaining tissue, with a lower proportion in the gills. The influx rates were dependent on the ambient metal concentration and were tissue-specific for each metal. The assimilation efficiency of trace metals in fish ingesting different prey (copepods, Artemia sp. and clam tissue) ranged from 6 to 24% for Cd, 32 to 68% for Se and 15 to 46% for Zn, and decreased with an increase in ingestion rate. The efflux rate constant of Cd in fish following uptake from the dietary phase (0.047 d -1 ) was higher than that following aqueous uptake (0.025 d -1 ), whereas the efflux rate constants of Se and Zn were comparable between these 2 exposure pathways. Our modeling calculations indicate that dietary uptake of Cd and Zn dominates their accumulation in fish when zooplankton are the main prey, whereas aqueous uptake may be the dominant pathway when planktivorous fish are the dominant prey for the predatory fish. Dietary uptake always dominates Se accumulation in these fish. The modeling results also indicated that the food chain transfer factor of Cd was < 0.5 in the fish regardless of the ingestion rate and the assimilation efficiency, consistent with the results of field studies. However, Se and Zn may potentially be biomagnified when the ingestion rate and assimilation efficiency are at the high end of the range possibly encountered by the predatory fish. KEY WORDS: Metals · Mangrove snapper · Exposure · Food chain · Uptake Resale or republication not permitted without written consent of the publisherMar Ecol Prog Ser 238: [173][174][175][176][177][178][179][180][181][182][183][184][185][186] 2002 2001). Organ specificity in metal accumulation may also be related to the metal speciation and the regulation of essential and non-essential metals (Hanson 1997, Baskin 2000, McGeer et al. 2000, Burgos & Rainbow 2001. Although most studies have quantified the metal concentrations in fish collected from the field, where they are exposed to metals both in the aqueous and in the dietary phases, it is difficult to quantitatively distinguish the metal uptake via food or water. Such information is useful for modeling metal transport within marine food chains and the interaction between fish and their environments.Recently, the kinetic modeling approach ha...

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Effects of chronic sublethal exposure to waterborne Cu, Cd or Zn in rainbow trout 2: tissue specific metal accumulation

Cited by 156 publications

References 22 publications

Effects of Cu on plasma cortisol and cortisol secretion by adrenocortical cells of rainbow trout (Oncorhynchus mykiss)

Effects of Cu on plasma cortisol and cortisol secretion by adrenocortical cells of rainbow trout (Oncorhynchus mykiss)

Cu Accumulation and Elimination in the Tissues of the Olive Flounder Paralichthys olivaceus

Exposure and potential food chain transfer factor of Cd, Se and Zn in marine fish Lutjanus argentimaculatus

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