Effects of chronic sleep deprivation on bone mass and bone metabolism in rats

Xu, Xianlin; Wang, Liang; Chen, Liying; Su, Tianjiao; Zhang, Yan; Wang, Tiantian; Ma, Weiguo; Yang, Fan; Zhai, Wujie; Xie, Yuanyuan; Li, Dan; Chen, Qiong; Fu, Xuemei; Ma, Yan

doi:10.1186/s13018-016-0418-6

Cited by 39 publications

(32 citation statements)

References 49 publications

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“…In that study, the lower levels of bone formation markers and higher levels of bone resorption markers resulted in lower BMD in sleep-restricted rats compared with controls [9]. Chronically sleep-deprived female rats had lower bone formation marker levels after 1 month and lower bone resorption markers after 3 months [10]. Over time, the chronically sleeprestricted rats had lower BMD and poorer bone microarchitecture compared with controls [10].…”

Section: Introductionmentioning

confidence: 81%

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Sleep duration and bone health measures in older men

et al. 2020

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The associations between objective measures of sleep duration and bone outcomes in older men are unknown. No consistent, significant association was identified between sleep duration and bone mineral density (BMD) in the current analysis. However, future research should determine if vitamin D status modifies this relationship. Introduction Prior studies, predominantly in women, reported that long and short self-reported sleep duration are associated with lower BMD. Associations between actigraphy-determined sleep duration and BMD or bone turnover markers (BTMs) in older men are unknown. Methods Men in The Osteoporotic Fractures in Men (MrOS) Study with wrist actigraphy and concurrent BMD assessment but without comorbidities affecting bone health were included. Sleep duration was considered as a continuous (N = 1926) and dichotomized variable where men were classified as getting the recommended (7-8 h/night; N = 478) or short (< 6 h/night; N = 577) sleep. The cross-sectional association between BMD, BTMs, and sleep duration was examined using a t test or linear regression, where appropriate, in unadjusted and adjusted models. Results There were no clinically or statistically significant differences in BMD at the L-spine, total hip, or femoral neck between men getting the recommended vs. short sleep duration, using actigraphy or self-reported sleep duration (all p ≥ 0.07). When sleep duration was considered as a continuous variable, femoral neck BMD was higher in men with longer self-reported sleep duration (β = 0.006 ±0.003, p = 0.02), but this was not significant after further adjustment. In men with low 25OHD (< 20 ng/mL), longer actigraphy-determined sleep duration was associated with higher total hip BMD (β = 0.016 ± 0.008; p = 0.04). Sleep duration and BTMs were not associated.

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Section: Introductionmentioning

confidence: 81%

“…Chronically sleep-deprived female rats had lower bone formation marker levels after 1 month and lower bone resorption markers after 3 months [10]. Over time, the chronically sleeprestricted rats had lower BMD and poorer bone microarchitecture compared with controls [10].…”

Section: Introductionmentioning

confidence: 90%

Sleep duration and bone health measures in older men

et al. 2020

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“…Similarly, other clinical surveys showed that patients with SD have lower BMD (9)(10)(11). An animal study showed that SD affects bone health by decreasing BMD and 25(OH)D (12). The study also demonstrated that SD affected BMD by decreasing osteogenesis and impairing mineralization of the newly formed bones.…”

Section: Introductionmentioning

confidence: 84%

Trehalose enhances bone fracture healing in a rat sleep deprivation model

Xu¹,

Wang²,

Sun³

et al. 2019

Ann. Transl. Med.

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Background: The purpose of this study was to investigate whether sleep deprivation (SD) could delay bone fracture healing and evaluate the therapeutic effect of trehalose. Methods: Eighteen 300-350 g female Sprague-Dawley rats were created a mid-femoral transverse osteotomy in the right thigh and divided into three groups (i.e., group 1: fracture; group 2: fracture + SD; and group 3: fracture + SD + trehalose). Seven days after surgery, the rats in group 2 and group 3 were started to get sleep-deprived for 18 h per day for 3 weeks. The rats in group 3 were injected with trehalose intraperitoneally at 1 g/kg/d for 3 weeks. Radiological and histological analyses were used to assess fracture healing quality. Circulating cytokines were detected by the end of the study. The expression of M1 and M2 macrophage markers were measured by quantitative real-time polymerase chain reaction (qPCR). Results: X-rays showed group 2 experienced much poorer fracture healing. Micro CT demonstrated that the bone quality of the fracture callus site in group 2 was much worse than that in groups 1 and 3. Both haematoxylin eosin (H&E) and Masson staining revealed that the bone fracture of the group 2 healed worse. Elisa results demonstrated that the interleukin-1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) of the rats in group 2 were significantly higher. In vitro study showed that 100 mM trehalose enhanced the expression of M2 macrophage markers (Arg-1 and IL-10), and decreased M1 macrophage polarization through the decreasing expression of IL-6. Conclusions: The present study showed (SD) could delay bone fracture healing in a rat model. And, trehalose could promote the healing of delayed bone fracture union by down-regulating pro-inflammatory mediators and enhancing M2 polarization.

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“…Significant relationships between depression, bone mineral density, and cortisol levels have been reported where depressed patients were shown to have higher cortisol levels and lower bone mineral density compared to placebo controls . Also, lifestyle that includes sleep disruption through shift work, sleep deprivation, physical strength, weight gain, and stress levels can adversely affect bone. Besides improving compliance, the positive effect of melatonin on mood and sleep may show benefits to bone by reducing cortisol levels and re‐entraining sleep/wake cycles to coincide with the light/dark cycle and natural bone rhythms.…”

Section: Discussionmentioning

confidence: 99%

“…Significant relationships between depression, bone mineral density, and cortisol levels have been reported, and so any improvement in any of these variables could also contribute to melatonin's beneficial effects on bone. Therefore, the daily diary logs kept by the MOPS cohort were further analyzed to provide additional data to explain melatonin's beneficial actions (eg, lifestyle, mood, sleep) on bone as described in other studies …”

Section: Introductionmentioning

confidence: 99%

Biological effects of melatonin on osteoblast/osteoclast cocultures, bone, and quality of life: Implications of a role for MT2 melatonin receptors, MEK1/2, and MEK5 in melatonin‐mediated osteoblastogenesis

Maria

Samsonraj

Munmun

et al. 2018

Journal of Pineal Research

127

164

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The Melatonin Osteoporosis Prevention Study (MOPS) demonstrated that nightly melatonin resulted in a time-dependent decrease in equilibrium ratios of serum osteoclasts and osteoblasts in perimenopausal women. This study examines mechanisms related to the ratios of osteoblasts and osteoclasts using coculture models (transwell or layered) of human mesenchymal stem cell (MSC) and human peripheral blood monocytes (PBMCs). Human MSC/PBMC cocultures exposed to melatonin in osteogenic (OS+) medium for 21 days induced osteoblast differentiation and mineralization; however, only in layered cocultures did melatonin inhibit osteoclastogenesis. Melatonin effects were mediated through MT2 melatonin receptors, MEK1/2, and MEK5. In layered but not transwell cocultures, melatonin increased OPG:RANKL ratios by inhibiting RANKL, suggesting that contact with osteoclasts during osteoblastogenesis inhibits RANKL secretion. Melatonin modulated expression of ERK1/2, ERK5, β1 integrin, GLUT4, and IRβ that was dependent upon the type of coculture; however, in both cultures, melatonin increased RUNX2 and decreased PPARγ expression, indicating a role for metabolic processes that control osteogenic vs adipogenic cell fates of MSCs. Furthermore, melatonin also has osteoblast-inducing effects on human adipose-derived MSCs. In vivo, one-year nightly melatonin (15 mg/L) given to neu female mice in their drinking water increased pErk1/2, pErk5, Runx2, and Opg and Rankl levels in bone consistent with melatonin's already reported bone-enhancing effects. Finally, analysis of daily logs from the MOPS demonstrated a significant improvement in mood and perhaps sleep quality in women receiving melatonin vs placebo. The osteoblast-inducing, bone-enhancing effects of melatonin and improvement in quality of life suggest that melatonin is a safe and effective bone loss therapy.

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Effects of chronic sleep deprivation on bone mass and bone metabolism in rats

Cited by 39 publications

References 49 publications

Sleep duration and bone health measures in older men

Sleep duration and bone health measures in older men

Trehalose enhances bone fracture healing in a rat sleep deprivation model

Biological effects of melatonin on osteoblast/osteoclast cocultures, bone, and quality of life: Implications of a role for MT2 melatonin receptors, MEK1/2, and MEK5 in melatonin‐mediated osteoblastogenesis

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