Effects of chronic sleep deprivation on glucose homeostasis in rats

Abstract

Epidemiological studies have shown that chronic sleep disturbances resulted in metabolic disorders. The purpose of this study was to assess the relationship between chronic sleep deprivation (CSD) and the glucose homeostasis in rats. Twenty-four rats were randomly divided into CSD group and control (CON) group. The CSD rats were intervened by a modified multiple platform method (MMPM) to establish an animal model of chronic sleep disturbances. After 3-month intervention, all rats were subjected to an intraperitoneal glucose tolerance test (IPGTT) and an insulin tolerance test (ITT), and the body weight, aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine, lipid profile group, and homeostasis model assessment-IR (HOMA-IR) were measured. Both the CSD and CON groups had an attenuation of weight gain after 3-month intervention. The plasma glucose level of CSD group was higher than that of the CON group during the IPGTT (P \ 0.01). The CSD rats showed a marked increase in HOMA-IR and ITT compared with the CON group (P \ 0.01). There were no significant differences of AST, ALT, creatinine, and most lipid parameters between the CSD and CON groups (P [ 0.05). The CSD has a marked effect on glucose homeostasis, comprising glucose intolerance and insulin resistance.

Proteomic Analysis of Rat Serum Revealed the Effects of Chronic Sleep Deprivation on Metabolic, Cardiovascular and Nervous System

“…The changes in these protein levels may imply the impairments of the normal energy metabolism in response to CSD. The current study demonstrated weight loss in the CSD animals ( Figure 4B); this is consistent with that of several other animal experiments [4] . It has been suggested that the CSD was linked to food intake, body weight and energy expenditure, metabolic syndrome and glucose homeostasis [4] .…”

“…The current study demonstrated weight loss in the CSD animals ( Figure 4B); this is consistent with that of several other animal experiments [4] . It has been suggested that the CSD was linked to food intake, body weight and energy expenditure, metabolic syndrome and glucose homeostasis [4] . Additionally, CSD rat models showed several metabolic alterations such as increased energy expenditure and intense catabolism.…”

Shift-work: is time of eating determining metabolic health? Evidence from animal models

“…Due to sleep deprivation, food ingestion was decreased (seven out of fifteen studies), or not changed (four out of fifteen), while in some studies (four studies) this behaviour was not monitored (Table 1). In the majority of the studies, a decrease in body weight gain was observed, which is suggested to be the result of increased energy expenditure (40) due to the exhausting conditions and physiological stress imposed by the extended protocols (from 18 to 20 h) of sleep deprivation (40)(41)(42)(43)(44)(45)(46)(47) . Studies observing a reduced body weight reported metabolic changes indicating a catabolic state or fasting like state, with reduced levels of glucose, low TAG, low cholesterol, low leptin levels, and in some studies accompanied by high levels of ghrelin and corticosterone (38,(40)(41)(42)(43)(44)(45)(46)(47)(48)(49)(50) .…”

“…Interestingly, the majority of studies here reported have used male animals and only two studies were found that explored the response in females (42,74) . In the study by Xu et al (42) , authors do not discuss a possible difference in their outcomes associated with the sex of the animals.…”

Stilbene Glucoside, a Putative Sleep Promoting Constituent from <i>Polygonum multiflorum</i> Affects Sleep Homeostasis by Affecting the Activities of Lactate Dehydrogenase and Salivary Alpha Amylase

“…1B), which is also associated with sleep situation. 11) More importantly, salivary alpha amylase is also responsive to extended waking. On the other hand, it is well established that brain metabolism is higher during wake and rapid eye movement (REM) sleep than that in nonrapid eye movement (NREM).…”