Effects of chronic parenteral pyridoxine and acute enteric tryptophan on pyridoxine status, glycemia and insulinemia stimulated by enteric glucose in weanling piglets

Matte, J. J.; Ponter, Andrew; Sève, Bernard

doi:10.4141/a97-013

Cited by 27 publications

(26 citation statements)

References 16 publications

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“…Effect of dietary tryptophan on the response of insulin and gastric inhibitory peptide (GIP) measured after meal in piglets (Ponter et al, 1994a); for individual time points, ⁎⁎⁎ designs statistical difference between low and control Trp at P b 0.001. plasma insulin when a physiological level of tryptophan was associated to a glucose load (Ponter et al, 1994a). Further results confirmed that supplemental tryptophan could help preventing this insulin resistance status in pigs under the condition of an adequate vitamin B6 status (Matte et al, 1997). Recently Zhang et al (2007) suggested that, in pigs, tryptophan effect on appetite could be mediated by ghrelin, a gut hormone involved in the regulation of food intake.…”

Section: Tryptophan Effect On Appetitementioning

confidence: 81%

Biological roles of tryptophan and its metabolism: Potential implications for pig feeding

2007

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Section: Tryptophan Effect On Appetitementioning

confidence: 81%

Biological roles of tryptophan and its metabolism: Potential implications for pig feeding

2007

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“…Measurements of Se were done using a fluorimetric method adapted by Giguère et al (2005) from the technique of Sheehan & Gao (1990) and P-5-P was determined using a fluorimetric method adapted by Matte et al (1997) from the technique of Srivastava & Beutler (1973). SeGPX activity was determined in blood plasma by the spectrophotometric method described by Gunzler & Flohe (1985).…”

Section: Laboratory Analysismentioning

confidence: 99%

Gene expression of porcine blastocysts from gilts fed organic or inorganic selenium and pyridoxine

Dalto¹,

Tsoi²,

Audet³

et al. 2015

Reproduction

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In this study, we determined how maternal dietary supplementation with pyridoxine combined with different sources of selenium (Se) affected global gene expression of porcine expanded blastocysts (PEB) during pregnancy. Eighteen gilts were randomly assigned to one of the three experimental diets (nZ6 per treatment): i) basal diet without supplemental Se or pyridoxine (CONT); ii) CONTC0.3 mg/kg of Na-selenite and 10 mg/kg of HCl-pyridoxine (MSeB 6 10); and iii) CONTC0.3 mg/kg of Se-enriched yeast and 10 mg/kg of HCl-pyridoxine (OSeB 6 10). All gilts were inseminated at their fifth post-pubertal estrus and killed 5 days later for embryo harvesting. A porcine embryospecific microarray was used to detect differentially gene expression between MSeB 6 10 vs CONT, OSeB 6 10 vs CONT, and OSeB 6 10 vs MSeB 6 10. CONT gilts had lower whole blood Se and erythrocyte pyridoxal-5-P concentrations than supplemented gilts (P!0.05). No treatment effect was observed on blood plasma Se-glutathione peroxidase activity (PZ0.57). There were 10, 247, and 96 differentially expressed genes for MSeB 6 10 vs CONT, OSeB 6 10 vs CONT, and OSeB 6 10 vs MSeB 6 10 respectively. No specific biological process was associated with MSeB 6 10 vs CONT. However, for OSeB 6 10 vs CONT, upregulated genes were related with global protein synthesis but not to selenoproteins. The stimulation of some genes related with monooxygenase and thioredoxin families was confirmed by quantitative real-time RT-PCR. In conclusion, OSeB 6 10 affects PEB metabolism more markedly than MSeB 6 10. Neither Se sources with pyridoxine influenced the Se-glutathione peroxidase metabolic pathway in the PEB, but OSeB 6 10 selectively stimulated genes involved with antioxidant defense.

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“…The 3 forms of vitamin B 6 , pyridoxamine (PAM), pyridoxal (PAL), and pyridoxine (PYR), were determined in duplicate by adaptation of methods described by Srivastava and Beutler (1973) and Matte et al (1997). Four stock solutions made in ultrapure water (500 μg/mL; Sigma-Aldrich) of PAM, PAL, PYR, and pyridoxal-5 -phosphate (P5P) were diluted (one-tenth for PAM, PAL, and P5P; one-thirtieth for PYR) to obtain standard solutions (respectively, 207, 246, 202, and 81 μM).…”

Section: Niacin (B3)mentioning

confidence: 99%

Effects of dietary nitrogen levels and carbohydrate sources on apparent ruminal synthesis of some B vitamins in dairy cows

Beaudet

Gervais

Graulet

et al. 2016

Journal of Dairy Science

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Effects of nitrogen level and carbohydrate source on apparent ruminal synthesis (ARS) of thiamin, riboflavin, niacin, vitamin B 6 , folates, and vitamin B 12 were evaluated using 4 lactating Holstein cows distributed in a 4 × 4 Latin square design with treatments following a 2 × 2 factorial arrangement. Cows were fitted with cannulas in the rumen and proximal duodenum. The treatments were 2 N levels and 2 carbohydrate sources. The diet with the high N level provided 14% crude protein, calculated to meet 110% of the protein requirements and an adequate supply in rumen-degradable protein, whereas the diet with the low N level contained 11% crude protein, calculated to meet 80% of the protein requirements with a shortage in rumen-degradable protein. Carbohydrate source treatments differed by their nature (i.e., high in starch from barley, corn, and wheat, or high in fiber from soybean hulls and dehydrated beet pulp). All 4 diets were isoenergetic, based on corn silage, and had the same forage-to-concentrate ratio (60:40, dry matter basis). Duodenal flow was determined using YbCl 3 as a marker. Each B-vitamin ARS was calculated as duodenal flow minus daily intake. The intake of several B vitamins varied among treatments, but because the animals consumed a similar amount of feed every day (average of 20 kg of dry matter/d) the difference was mostly due to vitamin content of each ingredient and their relative proportion in the diets. Decreasing N concentration in the diet reduced vitamin B 6 duodenal flow and increased its apparent ruminal degradation. It also decreased duodenal flow and ARS of folates. The high-starch diets increased duodenal flow and ruminal balance of riboflavin, vitamin B 6 , and folates, whereas the high-fiber diets increased vitamin B 12 ARS and duodenal flow. These effects on apparent synthesis are possibly due to changes in ruminal fermentation.

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Effects of chronic parenteral pyridoxine and acute enteric tryptophan on pyridoxine status, glycemia and insulinemia stimulated by enteric glucose in weanling piglets

Cited by 27 publications

References 16 publications

Biological roles of tryptophan and its metabolism: Potential implications for pig feeding

Biological roles of tryptophan and its metabolism: Potential implications for pig feeding

Gene expression of porcine blastocysts from gilts fed organic or inorganic selenium and pyridoxine

Effects of dietary nitrogen levels and carbohydrate sources on apparent ruminal synthesis of some B vitamins in dairy cows

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