Effects of chronic low-frequency stimulation on Ca2+-regulatory membrane proteins in rabbit fast muscle

Ohlendieck, Kay; Frömming, G. R.; Murray, Brendan E.; Maguire, Patricia; Leisner, Elmi; Traub, Irmtrud; Pette, Dirk

doi:10.1007/s004249900115

Cited by 20 publications

(10 citation statements)

References 28 publications

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“…Elevated SERCA3 expression (4.5‐fold at 3 h) postendurance exercise may be involved in restoring Ca 2+ homeostasis and/or may contribute to the calcium handling adaptations that occur in response to endurance training. Endurance training leads to adaptation in SERCA1/2 protein content in skeletal muscle, typically inducing a relative shift from the SERCA1 to SERCA2 isoform (75, 76). SERCA3 has not previously been examined after endurance exercise or endurance training, likely due to its low expression in skeletal muscle relative to the other isoforms.…”

Section: Discussionmentioning

confidence: 99%

Analysis of global mRNA expression in human skeletal muscle during recovery from endurance exercise

et al. 2005

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To search for novel transcriptional pathways that are activated in skeletal muscle after endurance exercise, we used cDNA microarrays to measure global mRNA expression after an exhaustive bout of high-intensity cycling (approximately 75 min). Healthy, young, sedentary males performed the cycling bout, and skeletal muscle biopsies were taken from the vastus lateralis before, and at 3 and 48 h after exercise. We examined mRNA expression in individual muscle samples from four subjects using cDNA microarrays, used repeated-measures significance analysis of microarray (SAM) to determine statistically significant expression changes, and confirmed selected results using real-time RT-PCR. In total, the expression of 118 genes significantly increased 3 h postcycling and 8 decreased. At 48 h, the expression of 29 genes significantly increased and 5 decreased. Many of these are potentially important novel genes involved in exercise recovery and adaptation, including several involved in 1) metabolism and mitochondrial biogenesis (FOXO1, PPARdelta, PPARgamma, nuclear receptor binding protein 2, IL-6 receptor, ribosomal protein L2, aminolevulinate delta-synthase 2); 2) the oxidant stress response (metalothioneins 1B, 1F, 1G, 1H, 1L, 2A, 3, interferon regulatory factor 1); and 3) electrolyte transport across membranes [Na+-K+-ATPase (beta3), SERCA3, chloride channel 4]. Others include genes involved in cell stress, proteolysis, apoptosis, growth, differentiation, and transcriptional activation, as well as all three nuclear receptor subfamily 4A family members (Nur77, Nurr1, and Nor1). This study is the first to characterize global mRNA expression during recovery from endurance exercise, and the results provide potential insight into 1) the transcriptional contributions to homeostatic recovery in human skeletal muscle after endurance exercise, and 2) the transcriptional contributions from a single bout of endurance exercise to the adaptive processes that occur after a period of endurance exercise training.

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Section: Discussionmentioning

confidence: 99%

Analysis of global mRNA expression in human skeletal muscle during recovery from endurance exercise

et al. 2005

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“…Indirect evidence points to the existence of at least fast and slow/cardiac isoforms of RyR and of the α‐subunits of DHPR (Péréon et al . 1997, Ohlendieck et al . 1999) Analyses on the specific distribution of these isoforms with regard to MHC‐based fibre types still need to be performed.…”

Section: Analytical Techniques For Single Fibre Analysismentioning

confidence: 99%

The impact of biochemical methods for single muscle fibre analysis

Pääsuke

Ereline

Gapeyeva

1999

Acta Physiologica Scandinavica

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106

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The neural and muscular changes during fatigue produced in repeated submaximal static contractions of knee extensors were measured. Three groups of differently adapted male subjects (power-trained, endurance-trained and untrained, 15 in each) performed the exercise that consisted of 10 trials of submaximal static contractions at the level of 40% of maximal voluntary contraction (MVC) force till exhaustion with the inter-trial rest intervals of 1 min. MVC force, reaction time and patellar reflex time components before and after the fatiguing exercise and following 5, 10 and 15 min of recovery were recorded. Endurance-trained athletes had a significantly longer holding times for all the 10 trials compared with power-trained athletes and untrained subjects. However, no significant differences in static endurance between power-trained athletes and untrained subjects were noted. The fatigue test significantly prolonged the time between onset of electrical and mechanical activity (electromechanical delay) in voluntary and reflex contractions. The electromechanical delay in voluntary contraction condition for power-trained and untrained subjects and in reflex condition for endurance-trained subjects had not recovered 15 min after cessation of exercise. No significant changes in the central component of visual reaction time (premotor time of MVC) and latency of patellar reflex were noted after fatiguing static exercise. It is concluded, that in this type of exercise the fatigue development may be largely owing to muscle contractile failure.

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“…An elevation of aerobic-oxidative capacity, decreased fibre calibre and changes in the density of distinct muscle proteins, switches in isoform expression patterns and alterations in proteinprotein interactions are established biochemical and cell biological hallmarks of transformed skeletal muscles. This includes: (i) metabolic pathways such as the citric acid cycle, fatty acid oxidation and the respiratory chain which causes a drastic increase in enzymes of aerobic substrate oxidation [39,40], (ii) the contractile apparatus that undergoes a stepwise replacement of myosin light and heavy chains from fast isoforms to their slower counterparts [41,42], (iii) the ion-regulatory machinery of the excitation-contractionrelaxation cycle with a shift from fast to slower isoforms with respect to the SERCA-type Ca 21 -ATPases, the voltagesensing dihydropyridine receptor, the ryanodine receptor Ca 21 -release channel and various Ca 21 -binding proteins [43,44], (iv) the neuromuscular junction with distinct changes in the acetylcholinesterase and the nicotinic acetylcholine receptor [45,46] and, (v) a decrease in the supramolecular interaction pattern between Ca…”

Section: Introductionmentioning

confidence: 99%

Proteomic profiling of chronic low‐frequency stimulated fast muscle

et al. 2007

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Skeletal muscle fibre transitions occur in many biological processes, in response to alterations in neuromuscular activity, in muscular disorders, during age-induced muscle wasting and in myogenesis. It was therefore of interest to perform a comprehensive proteomic profiling of muscle transformation. Chronic low-frequency stimulation of the rabbit tibialis anterior muscle represents an established model system for studying the response of fast fibres to enhanced neuromuscular activity under conditions of maximum activation. We have conducted a DIGE analysis of unstimulated control specimens versus 14-and 60-day conditioned muscles. A differential expression pattern was observed for 41 protein species with 29 increased and 12 decreased muscle proteins. Identified classes of proteins that are changed during the fast-to-slow transition process belong to the contractile machinery, ion homeostasis, excitation-contraction coupling, capillarization, metabolism and stress response. Results from immunoblotting agreed with the conversion of the metabolic, regulatory and contractile molecular apparatus to support muscle fibres with slower twitch characteristics. Besides confirming established muscle elements as reliable transition markers, this proteomics-based study has established the actin-binding protein cofilin-2 and the endothelial marker transgelin as novel biomarkers for evaluating muscle transformation.

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Effects of chronic low-frequency stimulation on Ca2+-regulatory membrane proteins in rabbit fast muscle

Cited by 20 publications

References 28 publications

Analysis of global mRNA expression in human skeletal muscle during recovery from endurance exercise

Analysis of global mRNA expression in human skeletal muscle during recovery from endurance exercise

The impact of biochemical methods for single muscle fibre analysis

Proteomic profiling of chronic low‐frequency stimulated fast muscle

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