Effects of chlorogenic acid on the liver cell metabolism under high glucose conditions

Abstract

This work aimed to investigate the potential of chlorogenic acid (CGA) to modulate the response of human hepatocytes under hyperglycemic condition. Markers of oxidative stress such as reduced glutathione content, malondialdehyde levels, advanced oxidation protein products, and nitric oxide production, as well as protein expression of Nrf2 transcription factor were measured in HepG2 cells cultured in normoglycemic (5.55 mM) and high-glucose medium (25 and 35 mM) pre-and post-treated with different concentrations of CGA (5, 10, 50 µM). Our results brought evidence of the protective effect of CGA on hepatic cells under hyperglycemic conditions that act through modulation of the intracellular reduced glutathione content and Nrf2 protein expression. The efficiency of CGA was more pronounced in the pre-treatment experiment. The findings of the present investigation demonstrated the protective role of CGA on hepatocytes by attenuating some markers of hyperglycemiamediated oxidative stress.

Zinc status, insulin resistance and glycoxidative stress in elderly subjects with type 2 diabetes mellitus

“…At the same time, the intracellular concentration of metallothioneins (MTs), the main intracellular proteins involved in Zn homeostasis, was shown to increase during ageing and age-related diseases (6,7). Zn deficiencies are reported in the course of ageing and in different diseases, such as DM, obesity, atherosclerosis, as well as rheumatoid arthritis or respiratory tract infections, among others (2,(8)(9)(10)(11)(12)(13)(14). In fact, the age-related changes of trace element concentrations in biological fluids/tissues are not only attributed to a deficient nutritional status, but also to an intrinsic dysregulated homeostasis (1).…”