Effects of chlorinated organics from wastewater treatment on algal growth

Simmons, Milagros S.; Sivaborvorn, Komol

doi:10.1007/bf01770039

Cited by 3 publications

(1 citation statement)

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“…A temporal and spatial collection strategy was employed and the highest lipid producer was screened and selected for further research [27]. The microalgal cultures were grown and maintained on BG-11 medium [32] 049. An aliquot of tetracycline (0.5 μl/ml) was added to the growth medium to prevent any bacterial contamination in the microalgal samples.…”

Section: Microalgal Strain and Growth Conditionsmentioning

confidence: 99%

The Utilization of Post-chlorinated Municipal Domestic Wastewater for Biomass and Lipid Production by Chlorella spp. Under Batch Conditions

Mutanda

Karthikeyan

Bux

2011

Appl Biochem Biotechnol

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South Africa has a rich microalgal biodiversity which has the potential to be used for renewable bio-fuel production in the region. Bioprospecting for oleaginous microalgae in KwaZulu Natal Province, South Africa, resulted in the establishment of a microalgal culture collection system for alternative energy research in the country. A potential hyper-lipid-producing Chlorella spp. strain was isolated, purified, and cultured in supplemented post-chlorinated wastewater for biomass and lipid production at the laboratory scale under batch mode. The microalgal strain was cultivated in different strengths of BG-11 media supplemented with wastewater from a local municipal domestic wastewater treatment plant. The Chlorella spp. was grown using ambient dissolved carbon dioxide in shake flasks under photosynthetically active radiation (±120 μmol m⁻² s⁻¹). Microalgal biomass and lipid productivity were monitored at 24-h intervals in the batch mode. The microalgal biomass was analyzed by direct light microscopy and indirectly by spectrophotometry at 600 nm, and the lipids were extracted and quantified. The growth rate of the Chlorella spp. was enhanced in post-chlorinated wastewater supplemented with 5 mM NaNO₃ with maximal biomass productivity. A dramatic increase in lipid yield was achieved with the post-chlorinated wastewater supplemented with 25 mM NaNO₃. Low dosages of free chlorine were found to enhance microalgal growth. These findings serve as a basis for further scale-up trials using municipal wastewater as a medium for microalgal biomass and lipid production.

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Section: Microalgal Strain and Growth Conditionsmentioning

confidence: 99%