Effects of Chloride Ion Binding on the Photochemical Properties of Salinibacter Sensory Rhodopsin I

Suzuki, Daisuke; Furutani, Yuji; Inoue, Kazuhide; Kikukawa, Takashi; Sakai, Makoto; Fujii, Masaaki; Kandori, Hideki; Homma, Michio; Sudo, Yuki

doi:10.1016/j.jmb.2009.06.050

Cited by 39 publications

(88 citation statements)

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“…A report [44] indicates that sensory rhodopsin has a binding site for Cl -, which regulates rhodopsin activity. Further, it has been reported that Cl -regulates mitochondrial ADP/ATP carrier by inhibiting the binding of adenine nucleotides to the carrier [45], and that ENaC activity in the kidney is regulated intracellular Cl - [33].…”

Section: Discussionmentioning

confidence: 99%

Chloride Ion Modulates Cell Proliferation of Human Androgen-independent Prostatic Cancer Cell

Hiraoka

Mitsuya

Niisato

et al. 2010

Cell Physiol Biochem

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In the present study, we investigated if the intracellular Cl^- affects cell growth and cell cycle progression of androgen-independent prostate cancer PC3 cells. PC3 cells cultured in a medium containing 113 mM Cl^- for 96 h grew up 9-fold in cell number, while PC3 cells cultured in an 8 mM-Cl^--containing culture medium showed complete arrest of cell growth even after culture for 96 h. Exposure of cells to the 8 mM-Cl^- culture medium diminished phosphorylation levels of Rb and cdc2, which are respectively key accelerators of transition from G₁ to S phase and G₂ to M phase in cell cycle progression. Culturing cells in the 8 mM-Cl^--containing culture medium upregulated the protein expression level of p21 (a CDK inhibitor) inhibiting transition of G₁ to S phase, and diminished the incorporation of 5-ethynyl-2′-deoxyuridine (EdU; a thymidine analogue) into DNA. These results suggest that cells cultured in the low Cl^- medium prolonged the duration of all phases of the cell cycle (G₁, S, and G₂/M), thereby abolishing overall cell cycle progression. Effects of culturing cells in the low Cl^- culture medium on cell cycle progression would be mediated via a change in the intracellular Cl^- concentration ([Cl^-]_i), since [Cl^-]_i was decreased under a low Cl^- culture medium. To clarify this possibility, we studied effects of furosemide and bumetanide, Na⁺/K⁺/2Cl^- cotransporter (NKCC) inhibitors, on proliferation of PC3 cells. Furosemide and bumetanide decreased [Cl^-]_i and cell growth of PC3 cells. These results suggest that a change in [Cl^-]_i would play a critical role in this growth mechanism.

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Section: Discussionmentioning

confidence: 99%

Chloride Ion Modulates Cell Proliferation of Human Androgen-independent Prostatic Cancer Cell

Hiraoka

Mitsuya

Niisato

et al. 2010

Cell Physiol Biochem

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“…In addition to this, we also performed similar experiments for SRI (17). From the results, we have found the key residues (factors) contributing to the color change from orange (ϳ500 nm) to purple (ϳ560 nm), as well as several unique factors, as the interaction between the Dand E-helices for SRII (16) or the chloride ion binding around the ␤-ionone ring for SRI (18), respectively.…”

mentioning

confidence: 82%

A Blue-shifted Light-driven Proton Pump for Neural Silencing

Sudo

Okazaki²,

Ono

et al. 2013

Journal of Biological Chemistry

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Background: Light-driven proton pumps are utilized to control the neural activity. Results: We have succeeded to produce a blue-shifted proton pump. The rotation of the ␤-ionone ring contributes to the spectral shift. Conclusion: The designed color variant provides a tool that allows the control of neural activity by blue light. Significance: The knowledge will help to understand the color-tuning mechanism and can be utilized for optogenetics.

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“…Previous studies have demonstrated that Cl À binds in other microbial rhodopsins, such as BR at low pH (<$2) [30], halorhodopsin [31] and sensory rhodopsin I [32]; however, the respective Cl À binding sites were different. Binding Cl À to these proteins changed their absorbance spectra.…”

Section: Changes In the Absorbance Spectra Of Npsrii When Binding A Cmentioning

confidence: 99%

The effects of chloride ion binding on the photochemical properties of sensory rhodopsin II from Natronomonas pharaonis

Tamogami

Iwano

Matsuyama

et al. 2014

Journal of Photochemistry and Photobiology B: Biology

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Effects of Chloride Ion Binding on the Photochemical Properties of Salinibacter Sensory Rhodopsin I

Cited by 39 publications

References 50 publications

Chloride Ion Modulates Cell Proliferation of Human Androgen-independent Prostatic Cancer Cell

Chloride Ion Modulates Cell Proliferation of Human Androgen-independent Prostatic Cancer Cell

A Blue-shifted Light-driven Proton Pump for Neural Silencing

The effects of chloride ion binding on the photochemical properties of sensory rhodopsin II from Natronomonas pharaonis

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