Effects of Chlorhexidine on the Structure and Permeability of Hamster Cheek Pouch Mucosa

Harvey, Brien V.; Squier, C. A.; Hall, B.K.

doi:10.1902/jop.1984.55.10.608

Cited by 20 publications

(6 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using hamster check pouch mucosa, it has been reported that intermittent applications (5 min daily for 3 weeks) of 0.2% CH produced no effeet, whereas 2.0% CH eaused epithelial hyperplasia and hyperkeratosis similar to that which we observed with continuous exposure to 0.2% CH for 3 days (13). It was also found that, in specimens exhibiting epithelial hyperkeratosis, there was an increase in the permeability barrier to tritiated water and horseradish peroxidase (13).…”

Section: Discussionsupporting

confidence: 82%

Effects on rat skin of exposure to saline and aqueous solutions of antibiotics and chlorhexidine

1986

View full text Add to dashboard Cite

Plastic chambers were glued to the dorsal skin of rats and were sealed after being filled with either distilled water, saline, an aqueous antibiotic mixture or aqueous chlorhexidine. The chambers were inspected at 24 h intervals and replenished. Groups of rats were killed at 24, 48 and 72 h; the chamber removed and the underlying skin excised and examined by light microscopy. The degree of dermatitis was assessed by means of an index of inflammation derived by scoring epidermal and dermal markers of inflammation. The results indicate that the antibiotic solution produced significantly less dermatitis compared with other treatments at each time interval studied. In general, epidermal ballooning and vesiculation occurred initially in the basal and prickle cells, but by 72 h the damaged cells were often replaced by intact keratinocytes, even though the skin was still immersed. Epidermal hyperplasia, as manifested by acanthosis and hyperkeratosis, was frequently present at 72 h.

show abstract

Section: Discussionsupporting

confidence: 82%

Effects on rat skin of exposure to saline and aqueous solutions of antibiotics and chlorhexidine

1986

View full text Add to dashboard Cite

show abstract

“…An interesting exception to this statement has recently been provided by the observation that the antimicrobial agent, ehlorhexidine. is capable of inducing a hyperplastic cheek pouch epithelium that does show re-dueed permeability (14). This effeet may refleet the strong binding of the divalent ehlorhexidine moleeule to eharged groups on the epithelial surfaee.…”

Section: Discussionmentioning

confidence: 98%

The permeability of hyperplastic oral epithelium

Squier

Hall

1985

J Oral Pathology Medicine

Self Cite

View full text Add to dashboard Cite

An epithelial hyperplasia is one of the reactions of skin and oral mucosa to chemical and mechanical insult. It is usually assumed that this reaction produces a more effective epithelial harrier, but there is no information as to whether a less permeable (issue results. To examine this question, hyperplasia was induced in the cheek pouches of hamsters by either chemical treatment with 0.0025% TPA in acetone or by mechanical abrasion with a rotating mop; untreated hamsters served as controls. The animals were killed and the cheek pouches were removed, mounted in diffusion chambers and the permeability to labelled water and horseradish peroxidase (IIRPO) determined. The results showed that higher values were obtained for the permeability constant of hyperplastic epithelia than for that of control tissue, suggesting that an increased epithelial thickness is not necessarily associated with an improved permeability barrier function. The presence of an inferior barrier layer in hyperplastic epithelia may be related to the increased rate of turnover of this tissue.

show abstract

“…Moreover, in an animal study, topical application of CHX was reported to result in its penetration through the epithelial barrier, causing tissue damage [10]. Withrow et al [32] reported CHX to be toxic for mouse lymphoma cells.…”

Section: Introductionmentioning

confidence: 99%

Monitoring of buccal epithelial cells by alkaline comet assay (single cell gel electrophoresis technique) in cytogenetic evaluation of chlorhexidine

Eren

Özmeriç

Şardaş

2002

Clinical Oral Investigations

View full text Add to dashboard Cite

During the last few years, there has been increasing interest in buccal epithelial cells for cytogenetic evaluation of different materials. In the present study, the use of these cells and peripheral lymphocytes for cytogenetic evaluation of chlorhexidine digluconate (CHX) with comet assay (single cell gel electrophoresis, or SCGE) is reported. This technique detects DNA strand breaks in individual cells in alkaline conditions. Thirteen volunteers were requested to rinse their mouths with 0.12% CHX solution for 18 days. Buccal epithelial cells and peripheral blood lymphocytes were obtained from all participants at baseline and the end of the experimental period. One hundred cells per subject were analysed for the DNA damage. A statistical increase was observed in the damaged buccal and blood cells after the CHX application. The mean grade of damage in buccal cells was statistically different from that in blood cells. Due to minimal absorption of chlorhexidine into the tissues and low concentrations of free chlorhexidine in the oral cavity, the DNA damage produced by chlorhexidine in lymphocytes was lower than in buccal epithelial cells. As chlorhexidine does not accumulate in the body, the frequencies of DNA damage could be transient. Detected DNA damage after CHX use might be the indication of an earlier effect, before DNA repair begins, and could be reversible.

show abstract

Effects of Chlorhexidine on the Structure and Permeability of Hamster Cheek Pouch Mucosa

Cited by 20 publications

References 14 publications

Effects on rat skin of exposure to saline and aqueous solutions of antibiotics and chlorhexidine

Effects on rat skin of exposure to saline and aqueous solutions of antibiotics and chlorhexidine

The permeability of hyperplastic oral epithelium

Monitoring of buccal epithelial cells by alkaline comet assay (single cell gel electrophoresis technique) in cytogenetic evaluation of chlorhexidine

Contact Info

Product

Resources

About