Effects of Chlorella vulgaris and Arthrospira platensis addition on viability of probiotic bacteria in yogurt and its biochemical properties

Beheshtipour, Hannane; Mortazavian, Amir Mohammad; Haratian, Parivash; Khosravi‐Darani, Kianoush

doi:10.1007/s00217-012-1798-4

Cited by 127 publications

(99 citation statements)

References 16 publications

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“…Recently, Beheshtipour et al (2012) have observed that the presence of C. vulgaris significantly increases the viability of L. acidophilus LA-5. Although we did not observe such a phenomenon for L. acidophilus CCM 4833, moderate growth stimulation was observed in our experiments for L. johnsonii CCM 4384 cultivated with 0.75-6 g/l of P. nurekis.…”

Section: Discussionmentioning

confidence: 99%

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Effect of green alga Planktochlorella nurekis on selected bacteria revealed antibacterial activity in vitro

Čermák¹,

Pražáková²,

Marounek³

et al. 2015

CZECH J ANIM SCI

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ABSTRACT:The green alga Planktochlorella nurekis (Chlorellaceae, Chlorophyta) is considered a producer of antibacterial mixture of long-chain fatty acids, which has possibly similar composition and mode of action as chlorellin produced by another green alga, Chlorella vulgaris. Although the antibacterial properties of C. vulgaris have been reported, the interactions of P. nurekis with bacteria have not been determined yet. The aim of this study was to elucidate the effect of P. nurekis water suspension on growth of selected gastrointestinal bacteria in vitro so that it could be used as a suitable feed supplement in animal farming. Unknown bacterial populations occurring in the algal suspension were identified using 16S rRNA sequencing assay. Selected strains were cultivated with lyophilized P. nurekis and the antibacterial effect was monitored. The composition of fatty acids and heat sensitivity of antibacterial substances were also examined. Sequencing analysis of 71 bacterial 16S rRNA genes in xenic algal suspension identified common environmental microbiota, one strain belonging to the class Alphaproteobacteria, 17 to Betaproteobacteria, 44 to Gammaproteobacteria (dominated by Pseudomonas putida strains), and nine to Sphingobacteria. The antimicrobial activity of P. nurekis suspension was tested at a concentration range of 0.75-6 mg/ml. The highest inhibitory effect was observed on bifidobacteria. Statistically significant reductions in bacterial counts were also observed for Escherichia coli, Salmonella enterica var. Enteritidis, S. enterica var. Infantis, Campylobacter jejuni, and Arcobacter butzleri. The growth of Lactobacillus johnsonii was significantly stimulated. The relative proportions of C14-C22 fatty acids in P. nurekis were found as follows: saturated 54.28%, monounsaturated 30.40%, and polyunsaturated 7.16%. The antibacterial compounds present in P. nurekis suspension exhibited thermostability. The results indicate that P. nurekis can inhibit some pathogenic gastrointestinal bacteria and seems to be a promising essential nutrients source in animal nutrition.

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Section: Discussionmentioning

confidence: 99%

“…Both microorganisms can act as competitors (e.g. for carbon sources), or microalgae can stimulate bacterial growth by providing increased nutrients for lactic acid bacteria and probiotic bacteria (Beheshtipour et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Effect of green alga Planktochlorella nurekis on selected bacteria revealed antibacterial activity in vitro

Čermák¹,

Pražáková²,

Marounek³

et al. 2015

CZECH J ANIM SCI

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“…The presence of spirulina biomass favoured the growth of the bacteria. Several authors (Varga et al 2002;Guldas and Irkin 2010;Beheshtipour et al 2012Beheshtipour et al , 2013Mazinani et al 2016) have tested the effect of spirulina biomass addition to yogurt, cheese, and fermented milk, with positive results, among which included an increase in the number of lactic acid bacteria and improvement of the nutritional quality of the fermented product during storage. However, to the best of our knowledge, the suitability of spirulina biomass as the sole substrate to obtain lactic acid-fermented products has not been investigated.…”

Section: Introductionmentioning

confidence: 99%

Lactic acid fermentation of Arthrospira platensis (spirulina) biomass for probiotic-based products

Niccolai

Shannon²,

Abu‐Ghannam³

et al. 2018

J Appl Phycol

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The first objective of this study was to evaluate the use of lyophilised biomass of the cyanobacterium Arthrospira platensis F&M-C256 as the sole substrate for lactic acid fermentation by the probiotic bacterium Lactobacillus plantarum ATCC 8014. After 48 h of fermentation, the bacterial concentration was 10.6 log CFU mL −1 and lactic acid concentration reached 3.7 g L −1. Lyophilised A. platensis F&M-C256 biomass was shown to be a suitable substrate for L. plantarum ATCC 8014 growth. The second objective of the study was to investigate whether lactic acid fermentation could enhance in vitro digestibility and antioxidant activity of A. platensis biomass. Digestibility increased by 4.4%, however it was not statistically significant, while the antioxidant activity and total phenolic content did increase significantly after fermentation, by 79% and 320% respectively. This study highlights the potential of A. platensis F&M-C256 biomass as a substrate for the production of probiotic-based products.

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“…Unfortunately till date, the biodiesel derived from microalgae could not be scaled-up to commercial marks. Chlorella could be established as an industrial strain of choice, as it is fast growing, may accumulate more than 50–70% lipids/gram of dry weight, its genome manipulation is accessible2122, and by large it is fit for human consumption232425. Primarily, after lipid extraction, the dilapidated biomass could be consumed in food industries2627.…”

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confidence: 99%

Real-time iTRAQ-based proteome profiling revealed the central metabolism involved in nitrogen starvation induced lipid accumulation in microalgae

Rai

Muthuraj

Gandhi

et al. 2017

Sci Rep

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To understand the post-transcriptional molecular mechanisms attributing to oleaginousness in microalgae challenged with nitrogen starvation (N-starvation), the longitudinal proteome dynamics of Chlorella sp. FC2 IITG was investigated using multipronged quantitative proteomics and multiple reaction monitoring assays. Physiological data suggested a remarkably enhanced lipid accumulation with concomitant reduction in carbon flux towards carbohydrate, protein and chlorophyll biosynthesis. The proteomics-based investigations identified the down-regulation of enzymes involved in chlorophyll biosynthesis (porphobilinogen deaminase) and photosynthetic carbon fixation (sedoheptulose-1,7 bisphosphate and phosphoribulokinase). Profound up-regulation of hydroxyacyl-ACP dehydrogenase and enoyl-ACP reductase ascertained lipid accumulation. The carbon skeletons to be integrated into lipid precursors were regenerated by glycolysis, β-oxidation and TCA cycle. The enhanced expression of glycolysis and pentose phosphate pathway enzymes indicates heightened energy needs of FC2 cells for the sustenance of N-starvation. FC2 cells strategically reserved nitrogen by incorporating it into the TCA-cycle intermediates to form amino acids; particularly the enzymes involved in the biosynthesis of glutamate, aspartate and arginine were up-regulated. Regulation of arginine, superoxide dismutase, thioredoxin-peroxiredoxin, lipocalin, serine-hydroxymethyltransferase, cysteine synthase, and octanoyltransferase play a critical role in maintaining cellular homeostasis during N-starvation. These findings may provide a rationale for genetic engineering of microalgae, which may enable synchronized biomass and lipid synthesis.

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Effects of Chlorella vulgaris and Arthrospira platensis addition on viability of probiotic bacteria in yogurt and its biochemical properties

Cited by 127 publications

References 16 publications

Effect of green alga Planktochlorella nurekis on selected bacteria revealed antibacterial activity in vitro

Effect of green alga Planktochlorella nurekis on selected bacteria revealed antibacterial activity in vitro

Lactic acid fermentation of Arthrospira platensis (spirulina) biomass for probiotic-based products

Real-time iTRAQ-based proteome profiling revealed the central metabolism involved in nitrogen starvation induced lipid accumulation in microalgae

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