Effects of Chitosan Concentration on the Protein Release Behaviour of Electrospun Poly(ε‐caprolactone)/Chitosan Nanofibers

Abstract

Poly( -caprolactone)/chitosan (PCL/chitosan) blend nanofibers with different ratios of chitosan were electrospun from a formic acid/acetic acid (FA/AA) solvent system. Bovine serum albumin (BSA) was used as a model protein to incorporate biochemical cues into the nanofibrous scaffolds. The morphological characteristics of PCL/chitosan and PCL/chitosan/BSA Nanofibers were investigated by scanning electron microscopy (SEM). Fourier transform infrared spectroscopy (FTIR) was used to detect the presence of polymeric ingredients and BSA in the Nanofibers. The effects of the polymer blend ratio and BSA concentration on the morphological characteristics and consequently on the BSA release pattern were evaluated. The average fiber diameter and pore size were greater in Nanofibers containing BSA. The chitosan ratio played a significant role in the BSA release profile from the PCL/chitosan/BSA blend. Nanofibrous scaffolds with higher chitosan ratios exhibited less intense bursts in the BSA release profile.

Fabrication of Porous Materials from Natural/Synthetic Biopolymers and Their Composites

“…In addition, compared to PCL/chitosan nanofibers, PCL/chitosan/BSA nanofibers showed higher fiber diameter and larger pore size. Results showed that the chitosan ratio affected significantly in the protein release profile from the PCL/chitosan/BSA blend [ 111 ].…”

Immobilization of Bovine Serum Albumin on the Chitosan/PVA Film

“…The spectrum of the Chitosan-g-PVA containing BSA in Figure 2 shows both peaks for BSA as well as the characteristic peaks for Chitosan-g-PVA without BSA, which confirmed the presence of BSA in the blends (Roozbahani et al 2014). …”

Binding of indocyanine green in polycaprolactone fibers using blend electrospinning for in vivo laser‐assisted vascular anastomosis

“…this stagnation could be an evidence for entrapment of the ICG in the PCL fibers, with the initial burst release due to ICG that was not entrapped but loosely attached to the surface of the fibers. A burst release was observed for other molecules entrapped in ICG fibers, such as BSA . The absorbance of the extracts from patches exposed to daylight showed a significantly lower ICG concentration than the samples stored in the dark.…”