Effects of chitosan-collagen dressing on wound healing in vitro and in vivo assays

Abstract

The present study was designed to fabricate a new chitosan-collagen sponge (CCS) for potential wound dressing applications. CCS was fabricated by a 3.0% chitosan mixture with a 1.0% type I collagen (7:3(w/w)) through freeze-drying. Then the dressing was prepared to evaluate its properties through a series of tests. The new-made dressing demonstrated its safety toward NIH3T3 cells. Furthermore, the CCS showed the significant surround inhibition zone than empty controls inoculated by E. coli and S. aureus. Moreover, the moisture rates of CCS were increased more rapidly than the collagen and blank sponge groups. The results revealed that the CCS had the characteristics of nontoxicity, biocompatibility, good antibacterial activity, and water retention. We used a full-thickness excisional wound healing model to evaluate the in vivo efficacy of the new dressing. The results showed remarkable healing at 14th day post-operation compared with injuries treated with collagen only as a negative control in addition to chitosan only. Our results suggest that the chitosan-collagen wound dressing were identified as a new promising candidate for further wound application.

Collagen-Chitosan Composite Powder as a Novel Biomaterial for Chronic Wounds in Hansen Disease-A Clinical Study

“…Bovine collagen is a promising resource of collagen biomaterial [16]. Chitosan has several inherent advantages [8] and can be crosslinked with collagen for various kinds of applications [2,7]. The use of biomaterials in wound healing has been studied as wound care is a major cause of concern for many years [7,12,13,[18][19][20]].…”

“…1c shows the effect of COL/CS on the size of the wound at time of admission (size 0) and discharge (size D). The average size of the wound while admission was 6.36 cm 2 and it significantly reduced to an average of 2.28 cm 2 during discharge. Meanwhile, for the control with application of T-Bact, the average size of the wound while admission was 6.78 cm 2 and it reduced to an average of 3.69 cm 2 during discharge.…”

“…Collagen, a structural protein in the connective tissues of animal origin, is an abundantly available natural biomaterial with inherent desirable properties. Collagen is a hydrophilic and a hemostatic biopolymer and is an extremely good substrate for cell adhesion and cell proliferation at the tissue site [2]. Collagenous biomaterials can be prepared in different forms as powder, solution, gel, fibre, sponge, film, sheet etc.…”

“…This necessitates the incorporation of other materials to the native collagen to achieve better scaffolding properties. Considering this, chitosan, a deacetylated form of collagen and a natural biopolymer has been crosslinked with collagen to result in a stable scaffold with desirable properties as a biomaterial composite with mechanical stability and similar biological properties [2,3,[5][6][7]. Moreover, chitosan has several advantages as a structural and biological biomaterial to promote hemostasis, anti-inflammation, platelet adhesion and aggregation, inhibit fibrinolysis, in the proliferation of granulation in wound tissues and as an antimicrobial agent [8].…”

“…Moreover, chitosan has several advantages as a structural and biological biomaterial to promote hemostasis, anti-inflammation, platelet adhesion and aggregation, inhibit fibrinolysis, in the proliferation of granulation in wound tissues and as an antimicrobial agent [8]. The applications of collagen (COL), chitosan (CS) biomaterials in wound healing are innumerous and COL/CS biomaterial has recently received potential significance as a promising candidate for wound healing [2,[7][8][9].…”