Effects of chitin and chitosan on root growth, biochemical defense response and exudate proteome of <i>Cannabis sativa</i>

Suwanchaikasem, Pipob; Nie, Shuai; Idnurm, Alexander; Selby‐Pham, Jamie; Walker, Robert; Boughton, Berin A.

doi:10.1002/pei3.10106

Cited by 10 publications

(13 citation statements)

References 84 publications

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“…The prediction parameters were set as default, and Phanerochaete chrysosporium was selected as a species identifier due to its closest relationship to A. rolfsii . MaxQuant protein search parameters and data filtering were set as described previously (Suwanchaikasem et al, 2023), with additional filtering criteria that the included proteins must be identified from at least half of the total number of replicates from at least one sample group. Data acquisition was applied without quantitative methods selected.…”

Section: Methodsmentioning

confidence: 99%

“…to induce C. sativa roots to secrete defense proteins into exudate (Suwanchaikasem et al, 2023). For A. rolfsii proteins, their functions were tentatively assigned based on BLAST results, where the full protein sequence was aligned against the NCBI fungal protein repository and the first-hit protein with the highest alignment score and annotated protein name was selected (Tables 1 and S1).…”

Section: Chitosan Induces C Sativa To Secrete Defense Proteins Into E...mentioning

confidence: 99%

“…The analysis was performed using the Triple-Quad 6410 LC-MS machine (Agilent Technologies, USA) equipped with Poroshell 120 EC-C18 column (2.7 μm; 2.1 Â 100 mm, Agilent Technologies, USA). The LC and MS parameters were set as described previously (Suwanchaikasem et al, 2023).…”

Section: Phytohormone Detectionmentioning

confidence: 99%

“…After concentration using an Amicon Ultra-15, 10 kDa MWCO centrifugal device (Merck Millipore, Germany), 100 μL of concentrated exudate proteins (approximately 5-11 μg protein) were aliquoted from each sample and processed with the S-Trap micro spin column (Protifi, USA) using the protocol previously described (Suwanchaikasem et al, 2023). Final peptides were loaded into the nano-LC-MS/MS system.…”

Section: Lc-ms/ms Analysis Of Exudate Proteomementioning

confidence: 99%

“…The eliciting effect of chitosan on the C. sativa root defense systems was recently reported. These results showed that .2% to .5% colloidal chitosan can induce production and secretion of defense proteins in root tissues and exudates (Suwanchaikasem et al, 2023). This was the impetus for further study to examine whether chitosan‐primed plants will be more resistant against fungal diseases.…”

Section: Introductionmentioning

confidence: 99%

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Hormonal and proteomic analyses of southern blight disease caused by Athelia rolfsii and root chitosan priming on Cannabis sativa in an in vitro hydroponic system

Suwanchaikasem,

Nie,

Selby‐Pham

et al. 2023

Plant Direct

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Southern blight disease, caused by the fungal pathogen Athelia rolfsii, suppresses plant growth and reduces product yield in Cannabis sativa agriculture. Mechanisms of pathology of this soil‐borne disease remain poorly understood, with disease management strategies reliant upon broad‐spectrum antifungal use. Exposure to chitosan, a natural elicitor, has been proposed as an alternative method to control diverse fungal diseases in an eco‐friendly manner. In this study, C. sativa plants were grown in the Root‐TRAPR system, a transparent hydroponic growth device, where plant roots were primed with .2% colloidal chitosan prior to A. rolfsii inoculation. Both chitosan‐primed and unprimed inoculated plants displayed classical symptoms of wilting and yellowish leaves, indicating successful infection. Non‐primed infected plants showed increased shoot defense responses with doubling of peroxidase and chitinase activities. The levels of growth and defense hormones including auxin, cytokinin, and jasmonic acid were increased 2–5‐fold. In chitosan‐primed infected plants, shoot peroxidase activity and phytohormone levels were decreased 1.5–4‐fold relative to the unprimed infected plants. When compared with shoots, roots were less impacted by A. rolfsii infection, but the pathogen secreted cell wall‐degrading enzymes into the root‐growth solution. Chitosan priming inhibited root growth, with root lengths of chitosan‐primed plants approximately 65% shorter than the control, but activated root defense responses, with root peroxidase activity increased 2.7‐fold along with increased secretion of defense proteins. The results suggest that chitosan could be an alternative platform to manage southern blight disease in C. sativa cultivation; however, further optimization is required to maximize effectiveness of chitosan.

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Section: Methodsmentioning

confidence: 99%

Section: Chitosan Induces C Sativa To Secrete Defense Proteins Into E...mentioning

confidence: 99%

Section: Phytohormone Detectionmentioning

confidence: 99%

Section: Lc-ms/ms Analysis Of Exudate Proteomementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Hormonal and proteomic analyses of southern blight disease caused by Athelia rolfsii and root chitosan priming on Cannabis sativa in an in vitro hydroponic system

Suwanchaikasem,

Nie,

Selby‐Pham

et al. 2023

Plant Direct

Self Cite

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The Impacts of Chitosan on Plant Root Systems and Its Potential to be Used for Controlling Fungal Diseases in Agriculture

Suwanchaikasem,

Idnurm,

Selby-Pham

et al. 2024

J Plant Growth Regul

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Chitosan is a natural elicitor, used for stimulating plant growth and inducing plant defense. However, due to difficulty in monitoring root growth and activity, the effects of chitosan treatment on plant root systems have been less studied as compared to plant shoot parts that include leaves, seeds, and fruits. This results in an indefinite outcome of the benefits of chitosan on plant roots. Therefore, this review aims to evaluate the effects of chitosan treatment on root growth and defense responses based on current evidence. Interestingly, many studies have demonstrated that chitosan can induce plant root defense systems, yet conversely inhibiting root growth. The effects were most clearly observed from studies using liquid or solid media as substrates, while the results from the studies using soil were inconclusive and require additional investigation to observe the effects of environmental factors. In addition, root chitosan treatment showed variable effects on shoot growth, where low chitosan concentrations tend to promote shoot growth, but high chitosan concentrations may affect shoot development. Additionally, this review discusses the potential methods of chitosan application onto plant roots. Water insolubility of chitosan is likely a major issue for root treatment. Chitosan can be dissolved in acids, but this could induce acidity stress in plant roots. Modified versions of chitosan, such as chitosan nanoparticles, carboxylated chitosan, and graft chitosan copolymers have been developed to improve solubility and functionality. Chitosan nanoparticles can also be used to encapsulate other biocontrol agents to augment biological effects on plant defense. In conclusion, root chitosan treatment could help to promote plant defense and prevent root infections, abating the uses of chemical fungicides in agriculture. However, further research is required to monitor the impact of root chitosan treatment on long-term plant growth in order to gain multifaceted information to maximize the effectiveness of root chitosan application.

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Evaluation of plumbagin synthesis: mimicking in vivo plant systems through the application of elicitors inducing stress on in vitro regenerated Plumbago zeylanica L.

Santra,

Chakraborty,

Ghosh

2024

Plant Cell Tiss Organ Cult

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Effects of chitin and chitosan on root growth, biochemical defense response and exudate proteome of Cannabis sativa

Cited by 10 publications

References 84 publications

Hormonal and proteomic analyses of southern blight disease caused by Athelia rolfsii and root chitosan priming on Cannabis sativa in an in vitro hydroponic system

Hormonal and proteomic analyses of southern blight disease caused by Athelia rolfsii and root chitosan priming on Cannabis sativa in an in vitro hydroponic system

The Impacts of Chitosan on Plant Root Systems and Its Potential to be Used for Controlling Fungal Diseases in Agriculture

Evaluation of plumbagin synthesis: mimicking in vivo plant systems through the application of elicitors inducing stress on in vitro regenerated Plumbago zeylanica L.

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