Effects of Chiral 3-Dichloroacetyl Oxazolidine on Glutathione S-Transferase and Antioxidant Enzymes Activity in Maize Treated with Acetochlor

Ye, Fei; Cao, Hai-Feng; Chen, Xuesong; Zhang, Meng; Fu, Ying; Li, Chunyan; Gao, Shuang

doi:10.15832/ankutbd.456671

Cited by 2 publications

(1 citation statement)

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“…The literature indicates that the content of glutathione (GSH) and the activity of GST in crops increase with the application of safeners. The binding of GSH and herbicides through nucleophilic substitution reactions is catalyzed by GST, and the formation of products without active chemical properties is considered to be a possible detoxification mechanism [10,11]. The application of herbicides significantly increases the probability of oxidative damage.…”

Section: Introductionmentioning

confidence: 99%

Protective Responses Induced by Chiral 3-Dichloroacetyl Oxazolidine Safeners in Maize (Zea mays L.) and the Detoxification Mechanism

et al. 2019

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Herbicide safeners selectively protect crops from herbicide injury while maintaining the herbicidal effect on the target weed. To some extent, the detoxification of herbicides is related to the effect of herbicide safeners on the level and activity of herbicide target enzymes. In this work, the expression of the detoxifying enzyme glutathione S-transferase (GST) and antioxidant enzyme activities in maize seedlings were studied in the presence of three potential herbicide safeners: 3-dichloroacetyl oxazolidine and its two optical isomers. Further, the protective effect of chiral herbicide safeners on detoxifying chlorsulfuron in maize was evaluated. All safeners increased the expression levels of herbicide detoxifying enzymes, including GST, catalase (CAT), and peroxidase (POD) to reduce sulfonylurea herbicide phytotoxicity in maize seedlings. Our results indicate that the R-isomer of 3-(dichloroacetyl)-2,2,5-trimethyl-1,3-oxazolidine can induce glutathione (GSH) production, GST activity, and the ability of GST to react with the substrate 1-chloro-2,4-dinitrobenzene (CDNB) in maize, meaning that the R-isomer can protect maize from damage by chlorsulfuron. Information about antioxidative enzyme activity was obtained to determine the role of chiral safeners in overcoming the oxidative stress in maize attributed to herbicides. The interaction of safeners and active target sites of acetolactate synthase (ALS) was demonstrated by molecular docking modeling, which indicated that both isomers could form a good interaction with ALS. Our findings suggest that the detoxification mechanism of chiral safeners might involve the induction of the activity of herbicide detoxifying enzymes as well as the completion of the target active site between the safener and chlorsulfuron.

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Section: Introductionmentioning

confidence: 99%