Effects of Chinese Jianpi herbs on cell apoptosis and related gene expression in human gastric cancer grafted onto nude mice

Abstract

The inhibition of gastric cancer cell growth in vivo by Chinese Jianpi herbs and SRRS is related to induction of the cell apoptosis which may be involved in aberrant expression of p53 and bcl-2 genes.

Apigenin Induces Apoptosis and Blocks Growth of Medroxyprogesterone Acetate-Dependent BT-474 Xenograft Tumors

“…Wang et al [31] reported similar findings in colorectal cancer xenografts. Apoptosis is also induced by other tumor growthinhibiting phytochemicals [32] and anticancer drugs such as 5-flurouracil [33] and cisplatin [34]. Previous studies demonstrating induction of apoptosis by apigenin suggested that the following mechanisms may be involved: induction of caspase 3 [35], induction/activation of p53 [36], up-regulation of FADD [31], and, in MDA-MB-435 cells, stimulation of proteasome-dependent degradation of Her2/neu [27].…”

Biotherapy Effect of Shenfuweikang Herbs on Gastric Carcinoma

“…Similar to the other malignant tumors, gastric carcinoma is always accompanying with abnormal cell proliferation and differentiation, as well as with abnormal apoptosis [7,8]. The enhanced induction of apoptosis in human gastric carcinoma cells can be observed after treatment with 5-Fluorouracil, Cisplatin, arsenous oxide and so on [9,10]. These data suggest that inducing cancer cell apoptosis should be a therapeutic method for gastric carcinoma.…”

Effects of Wei Chang An on expression of multiple genes in human gastric cancer grafted onto nude mice

“…Wolf, Glycyrrhiza uralensis Fisch., Rehd. Et Wils., and Prunella vulgaris L. The preparation of WCA decoction has been described previously [8,9] and the concentration of the decoction was 120 g/L. High Performance Liquid Chromatography (HPLC) was used for monitoring the stability of the decoction.…”

“…Previous studies have shown the tumor suppressive effect of WCA involved multiple biological processes and mechanisms, including enhanced a p o p to sis a n d in h ib itio n o f proliferation. However, further mechanisms are not fully understood [9][10][11][12] .…”

“…Immunohistochemical staining for Ki-67 has shown WCA inhibits cell proliferation. The terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate f luorescence nick end labeling (TUNEL) method, flow cytometry and electron microscopy have clarified that WCA also enhances apoptosis [9] . In the orthotopic implanted model, inhibition of tumor growth and metastasis has also been verified, and higher activity of NK cells has been found in WCA-treated animals compared with the control group [10][11][12] .…”