Effects of chilling to 0°C on the morphology of meiotic spindles in human metaphase II oocytes

Zenzes, Maria Teresa; Bielecki, Ryszard; Casper, Robert F.; Leibo, S.P.

doi:10.1016/s0015-0282(00)01800-8

Cited by 156 publications

(67 citation statements)

References 43 publications

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“…As mentioned above, in vitro development of parthenogenetic embryos is a good indicator of the viability of vitrified oocytes. However, oocytes are generally prone to disruption of the metaphase spindle during cooling, which may lead to chromosomal segregation aberrations and aneuploidy in embryos, although such disruption is more reversible in mice than in bovine or human oocytes [32][33][34]. According to previous studies, embryonic aneuploidy does not always compromise preimplantation development, but does impair postimplantation development [35,36].…”

Section: Discussionmentioning

confidence: 99%

The Developmental Ability of Vitrified Oocytes from Different Mouse Strains Assessed by Parthenogenetic Activation and Intracytoplasmic Sperm Injection

et al. 2007

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Abstract. Assessment of the developmental ability of oocytes following freezing and thawing is an important step for optimizing oocyte cryopreservation techniques. However, the in vitro fertilization of frozen-thawed mouse oocytes is often inefficient because of incomplete capacitation of spermatozoa in the absence of surrounding cumulus cells. This study was undertaken to determine whether the oocyte cryopreservation efficiency of different strains of mice could be assessed from the development of oocytes following parthenogenetic activation and intracytoplasmic sperm injection (ICSI). Oocytes were collected from hybrid (C57BL/6 × DBA/2) F1 or inbred (C57BL/6J, C3H/HeN, DBA/2J and BALB/cA) strains and were vitrified in a solution containing ethylene glycol, DMSO, Ficoll and sucrose. In the first series of experiments, oocytes were activated parthenogenetically by Sr 2+ treatment after warming. The oocytes from the inbred strains, but not those of the F1 hybrid, were diploidized by cytochalasin treatment to obtain a sufficient number of blastocysts. In all strains tested, parthenogenetic embryos derived from vitrified oocytes developed into blastocysts at rates between 23 and 68%. In the second series of experiments, vitrified oocytes from each strain were injected with homologous spermatozoa after warming. Normal offspring were obtained from all strains at rates between 5 and 26% per embryo transferred. Thus, the feasibility of oocyte cryopreservation protocols can be assessed easily by in vitro development of parthenogenetic embryos or by in vivo development of ICSI embryos. Moreover, the oocytes of these four major inbred strains of mice can be cryopreserved safely for production of offspring. Key words: Intracytoplasmic sperm injection (ICSI), Mouse, Oocyte, Parthenogenetic development, Vitrification (J. Reprod. Dev. 53: [1199][1200][1201][1202][1203][1204][1205][1206] 2007) ryopreservation of embryos and gametes is a major strategy for genetic conservation of mammalian species. In laboratory mice, this technique is also important to preserve invaluable genetic resources from naturally occurring mutant mice and to save costs and space for storage of genetically engineered mice, the number of which is expanding very rapidly. Thanks to intensive technical development during recent decades, mouse oocytes [1,2], spermatozoa [3,4] and embryos [5][6][7] can now be cryopreserved successfully using appropriate methods. However, as most of these

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Section: Discussionmentioning

confidence: 99%

The Developmental Ability of Vitrified Oocytes from Different Mouse Strains Assessed by Parthenogenetic Activation and Intracytoplasmic Sperm Injection

et al. 2007

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“…Reasons for this may include low permeability of the oocyte membrane to cryoprotectants, susceptibility of the meiotic spindle to cooling [5][6][7][8][9], and toxic effects of cryoprotectants that affect various aspects of the oocyte's physiology [3,7,10,11]. The two main methods that have been used for cryopreservation are slow freezing/thawing and ultrarapid freezing (vitrification).…”

Section: Introductionmentioning

confidence: 99%

Maturation, Fertilization, and the Structure and Function of the Endoplasmic Reticulum in Cryopreserved Mouse Oocytes1

Lowther

Weitzman

Maier

et al. 2009

Biology of Reproduction

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Oocyte cryopreservation is a promising technology that could benefit women undergoing assisted reproduction. Most studies examining the effects of cryopreservation on fertilization and developmental competence have been done using metaphase IIstage oocytes, while fewer studies have focused on freezing oocytes at the germinal vesicle (GV) stage, followed by in vitro maturation. Herein, we examined the effects of vitrifying GVstage mouse oocytes on cytoplasmic structure and on the ability to undergo cytoplasmic changes necessary for proper fertilization and early embryonic development. We examined the endoplasmic reticulum (ER) as one indicator of cytoplasmic structure, as well as the ability of oocytes to develop Ca 2+ release mechanisms following vitrification and in vitro maturation. Vitrified GV-stage oocytes matured in culture to metaphase II at a rate comparable to that of controls. These oocytes had the capacity to release Ca 2+ following injection of inositol 1,4,5-trisphosphate, demonstrating that Ca 2+ release mechanisms developed during meiotic maturation. The ER remained intact during the vitrification procedure as assessed using the lipophilic fluorescent dye DiI. However, the reorganization of the ER that occurs during in vivo maturation was impaired in oocytes that were vitrified before oocyte maturation. These results show that vitrification of GV-stage oocytes does not affect nuclear maturation or the continuity of the ER, but normal cytoplasmic maturation as assessed by the reorganization of the ER is disrupted. Deficiencies in factors that are responsible for proper ER reorganization during oocyte maturation could contribute to the low developmental potential previously reported in vitrified in vitro-matured oocytes.assisted reproductive technology, gamete biology, in vitro fertilization, meiosis

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“…In MII oocytes these parameters are further confounded by the presence of a fragile cytoskeleton that is resistant to the volumetric excursions commonly associated with equilibrium freezing and a highly temperature sensitive meiotic spindle apparatus (Saragusty and Arav, 2011). These time-dependent sensitivities to chilling to 0°C are evident in MII oocytes from a range of species including mouse (Pickering and Johnson, 1987) rhesus monkey and human (Pickering et al, 1990;Zenzes et al, 2001). Indeed, cooling oocytes to room temperature for even as little as 10 minutes can cause irreversible damage to the meiotic spindle.…”

Section: Cryobiology and Fertility Preservationmentioning

confidence: 99%

Preservation of female fertility in humans and animal species

Picton¹

2018

Anim Reprod

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A detailed understanding of the cryobiology of gametes and complex tissues has led to the development of methods that facilitate the successful low temperature banking of isolated mature human oocytes, or immature oocytes in situ within fragments of human ovarian cortex. Although many outstanding research challenges remain to be addressed, the successful development of new treatments to preserve female fertility for a range of clinical indications has largely been underpinned by the conduct of extensive, fundamental research on oocytes and ovarian tissues from a number of laboratory and commercially important farm species. Indeed, the most recent evidence from large animals suggests that it is also possible to cryopreserve intact whole ovaries along with their supporting vasculature for later autotransplantation and restoration of natural fertility. This review will explore how the methods developed to preserve human oocytes and ovarian tissues can now be used strategically to support the development of conservation strategies aimed at safeguarding the genetic diversity of commercially important domestic animals and also of preserving the female germplasm for wild animals and endangered species.

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Effects of chilling to 0°C on the morphology of meiotic spindles in human metaphase II oocytes

Cited by 156 publications

References 43 publications

The Developmental Ability of Vitrified Oocytes from Different Mouse Strains Assessed by Parthenogenetic Activation and Intracytoplasmic Sperm Injection

The Developmental Ability of Vitrified Oocytes from Different Mouse Strains Assessed by Parthenogenetic Activation and Intracytoplasmic Sperm Injection

Maturation, Fertilization, and the Structure and Function of the Endoplasmic Reticulum in Cryopreserved Mouse Oocytes1

Preservation of female fertility in humans and animal species

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