Effects of Chelating Reagents on Colonial Appearance of Paenibacillus alvei Isolated from Canine Oral Cavity

Fujihara, Masatoshi; Maeda, Kouji; Sasamori, Eriko; Matsushita, Mitsugu; Harasawa, Ryô

doi:10.1292/jvms.71.147

Cited by 6 publications

(5 citation statements)

References 46 publications

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“…A human case report proposed that an undrained dental abscess could have been a source of persistent bacteremia by P. amylolyticus and Lysinibacillus. 34 Paenibacilli were also isolated from the subgingival dental plaque of a human gingivitis lesion 24 and the oral cavity of a healthy dog, 14 further supporting our hypothesis. Paenibacillus spp.…”

supporting

confidence: 75%

Paenibacillus amylolyticus osteomyelitis in a Poodle dog: case report and literature review

Rampacci

Sforna

Dentini³

et al. 2022

J VET Diagn Invest

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Paenibacilli are gram-variable, endospore-forming bacteria that occupy various ecologic niches. These microorganisms have been known to infect humans occasionally at various anatomic sites. However, in humans, as well as in other vertebrate animals, the relationship between disease and isolation of Paenibacillus spp. remains poorly understood. We report here a case of infection in an adult Poodle dog. The animal had nodules in the lungs and multifocal osteolytic expansile bone lesions. From bone, Paenibacillus amylolyticus was recovered by culture and identified by MALDI-TOF mass spectroscopy and 16S rDNA sequencing; pyogranulomatous inflammation was observed in lung and bone specimens. The microorganism was resistant to clindamycin and imipenem. Four-month treatment with amoxicillin–clavulanate resulted in clinical resolution of disease in this dog. Nevertheless, therapy for more prolonged periods should be considered because recurrent infections can occur as a result of the transition of Paenibacillus spores to vegetative cells. Disease caused by a Paenibacillus species has not been reported previously in dogs, to our knowledge.

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supporting

confidence: 75%

Paenibacillus amylolyticus osteomyelitis in a Poodle dog: case report and literature review

Rampacci

Sforna

Dentini³

et al. 2022

J VET Diagn Invest

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“…For example, Paenibacillus vortex forms both wandering and rotating clusters when cells elongate in the presence of mitomycin C [ 12 ] or when they are co-cultivated with Escherichia coli [ 13 ]. Paenibacillus Alvei also forms wandering colonies [ 14 ], while Paenibacillus sp. NAIST15–1 forms both wandering and rotating colonies [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Scattered migrating colony formation in the filamentous cyanobacterium, Pseudanabaena sp. NIES-4403

et al. 2021

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Background Bacteria have been reported to exhibit complicated morphological colony patterns on solid media, depending on intracellular, and extracellular factors such as motility, cell propagation, and cell-cell interaction. We isolated the filamentous cyanobacterium, Pseudanabaena sp. NIES-4403 (Pseudanabaena, hereafter), that forms scattered (discrete) migrating colonies on solid media. While the scattered colony pattern has been observed in some bacterial species, the mechanism underlying such a pattern still remains obscure. Results We studied the morphology of Pseudanabaena migrating collectively and found that this species forms randomly scattered clusters varying in size and further consists of a mixture of comet-like wandering clusters and disk-like rotating clusters. Quantitative analysis of the formation of these wandering and rotating clusters showed that bacterial filaments tend to follow trajectories of previously migrating filaments at velocities that are dependent on filament length. Collisions between filaments occurred without crossing paths, which enhanced their nematic alignments, giving rise to bundle-like colonies. As cells increased and bundles aggregated, comet-like wandering clusters developed. The direction and velocity of the movement of cells in comet-like wandering clusters were highly coordinated. When the wandering clusters entered into a circular orbit, they turned into rotating clusters, maintaining a more stable location. Disk-like rotating clusters may rotate for days, and the speed of cells within a rotating cluster increases from the center to the outmost part of the cluster. Using a mathematical modeling with simplified assumption we reproduced some features of the scattered pattern including migrating clusters. Conclusion Based on these observations, we propose that Pseudanabaena forms scattered migrating colonies that undergo a series of transitions involving several morphological patterns. A simplified model is able to reproduce some features of the observed migrating clusters.

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“…For example, Paenibacillus vortex forms both wandering and rotating clusters when cells elongate in the presence of mitomycin C [7] or when they are co-cultivated with E.coli [15]. Paenibacillus alvei also forms wandering colonies [16], while Paenibacillus sp. NAIST15-1 forms both wandering and rotating colonies [8].…”

Section: Introductionmentioning

confidence: 99%

Scattered migrating colony formation in the filamentous cyanobacterium, Pseudanabaena sp. NIES-4403

Yamamoto

Fukasawa

Shoji

et al. 2020

Preprint

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Background Bacteria have been reported to exhibit complicated morphological colony patterns on solid media, depending on intracellular, and extracellular factors such as motility, cell propagation, and cell-cell interaction. We isolated the filamentous cyanobacterium, Pseudanabaena sp. NIES-4403 (Pseudanabaena, hereafter), that forms scattered (discrete) migrating colonies on solid media. While the scattered colony pattern has been observed in some bacterial species, the mechanism underlying such a pattern still remains obscure. Results We studied the morphology of Pseudanabaena migrating collectively and found that this species forms randomly scattered clusters varying in size and further consists of a mixture of comet-like wandering clusters and rotating disks. Quantitative analysis of the formation of these wandering and rotating clusters showed that bacterial filaments tend to follow trajectories of previously migrating filaments at velocities that are dependent on filament length. Collisions between filaments occurred without crossing paths, which enhanced their nematic alignments, giving rise to bundle-like colonies. As cells increased and bundles aggregated, comet-like wandering clusters developed. The direction and velocity of the movement of cells in comet-like wandering clusters were highly coordinated. When the wandering clusters entered into a circular orbit, they turned into rotating disks, maintaining a more stable location. Disks may rotate for days, and the speed of cells within a rotating disk increases from the center to the outmost part of the disk. Using a minimal agent-based model, we reproduced some features of Pseudanabaena migrating clusters. Conclusion Based on these observations, we propose that Pseudanabaena forms scattered migrating colonies that undergo a series of transitions involving several morphological patterns. A minimal agent-based model is able to reconstruct some features of the observed migrating clusters.

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Effects of Chelating Reagents on Colonial Appearance of Paenibacillus alvei Isolated from Canine Oral Cavity

Cited by 6 publications

References 46 publications

Paenibacillus amylolyticus osteomyelitis in a Poodle dog: case report and literature review

Paenibacillus amylolyticus osteomyelitis in a Poodle dog: case report and literature review

Scattered migrating colony formation in the filamentous cyanobacterium, Pseudanabaena sp. NIES-4403

Scattered migrating colony formation in the filamentous cyanobacterium, Pseudanabaena sp. NIES-4403

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