Effects of ceragenins and conventional antimicrobials on Candida albicans and Staphylococcus aureus mono and multispecies biofilms

Hacıoğlu, Mayram; Hacıosmanoğlu, Ebru; Birteksöz-Tan, Ayşe Seher; Bozkurt-Guzel, Cagla; Savage, Paul B.

doi:10.1016/j.diagmicrobio.2019.06.014

Cited by 23 publications

(16 citation statements)

References 38 publications

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“…albicans and S. aureus can be co-isolated from a number of infections and exhibit enhanced disease severity and morbidity compared to single species infection [26]. The synergy between C. albicans and S. aureus contributes to the recalcitrance of polymicrobial biofilm communities, which has proven to be very resistant to a range of antibiotics, highlighting the emergent need for new antibiofilm agents [27]. Here, we first showed that sanguinarine imparted a cell growth-inhibitory response in mono-and dual-species planktic cells of C. albicans SC5314 and S. aureus CMCC26003.…”

Section: Discussionmentioning

confidence: 99%

RETRACTED: Sanguinarine Inhibits Mono- and Dual-Species Biofilm Formation by Candida albicans and Staphylococcus aureus and Induces Mature Hypha Transition of C. albicans

et al. 2020

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Previous studies have reported that sanguinarine possesses inhibitory activities against several microorganisms, but its effects on mono- and dual-species biofilms of C. albicans and S. aureus have not been fully elucidated. In this study, we aimed to evaluate the efficacy of sanguinarine for mono- and dual-species biofilms and explore its ability to induce the hypha-to-yeast transition of C. albicans. The results showed that the minimum inhibitory concentration (MIC) and minimum biofilm inhibitory concentration (MBIC90) of sanguinarine against C. albicans and S. aureus mono-species biofilms was 4, and 2 μg/mL, respectively, while the MIC and MBIC90 of sanguinarine against dual-species biofilms was 8, and 4 μg/mL, respectively. The decrease in the levels of matrix component and tolerance to antibiotics of sanguinarine-treated mono- and dual-species biofilms was revealed by confocal laser scanning microscopy combined with fluorescent dyes, and the gatifloxacin diffusion assay, respectively. Meanwhile, sanguinarine at 128 and 256 μg/mL could efficiently eradicate the preformed 24-h biofilms by mono- and dual-species, respectively. Moreover, sanguinarine at 8 μg/mL could result in the transition of C. albicans from the mature hypha form to the unicellular yeast form. Hence, this study provides useful information for the development of new agents to combat mono- and dual-species biofilm-associated infections, caused by C. albicans and S. aureus.

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Section: Discussionmentioning

confidence: 99%

RETRACTED: Sanguinarine Inhibits Mono- and Dual-Species Biofilm Formation by Candida albicans and Staphylococcus aureus and Induces Mature Hypha Transition of C. albicans

et al. 2020

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“…CSAs possess potent antimicrobial activities against a broad spectrum of microorganisms including Gram-positive bacteria, Gram-negative bacteria, fungi, virus, or parasites. 21,37 According to the literature, CSAs may be effective, ie, against hypervirulent Pseudomonas aeruginosa strain LESB58, colistin-resistant K. pneumoniae, carbapenem resistant Acinetobacter baumannii, methicillin, or even vancomycin resistant Staphylococcus aureus. In turn, among fungi, CSAs can be useful against fluconazole-resistant Candida albicans or C. auris.…”

Section: Discussionmentioning

confidence: 99%

“…16,25,42 Additionally, the ability of CSAs to modulate mixed species biofilms formed by organisms like P. aeruginosa and S. aureus or C. albicans and S. aureus was observed. 37,43 Additionally, Durnaś et al 19, demonstrated the significant inhibition of biofilm generation by fungi as C. albicans. In addition, data reported by Hashemi et al 39 indicate antibiofilm activities of CSAs against C. auris.…”

Section: Discussionmentioning

confidence: 99%

“…Previous results suggested that the mechanism of ceragenins action is the damage to the plasma membrane leading to its dysfunction. 16,21,37 In order to confirm this hypothesis with regard to MDR bacteria such as NDM-1 producing strains, NPN uptake and ONPG hydrolysis assays were used. We found that all analyzed CSAs were able to permeabilize the outer membrane of NDM-1 producing bacteria in a dose-dependent manner.…”

Section: Discussionmentioning

confidence: 99%

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<p>NDM-1 Carbapenemase-Producing Enterobacteriaceae are Highly Susceptible to Ceragenins CSA-13, CSA-44, and CSA-131</p>

Chmielewska¹,

Skłodowski²,

Piktel³

et al. 2020

IDR

Self Cite

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Background and Purpose: Treatment of infections caused by NDM-1 carbapenemaseproducing Enterobacteriaceae (CPE) represents one of the major challenges of modern medicine. In order to address this issue, we tested ceragenins (CSAs-cationic steroid antimicrobials) as promising agents to eradicate various NDM-1-producing Gram-negative enteric rods. Materials and Methods: Susceptibility to CSA-13, CSA-44, and CSA-131 of four reference NDM-1 carbapenemase-producing strains, ie, Escherichia coli BAA-2471, Enterobacter cloacae BAA-2468, Klebsiella pneumoniae subsp. pneumoniae BAA-2472, and K. pneumoniae BAA-2473 was assessed by MIC/MBC testing of planktonic cells as well as biofilm formation/disruption assays. To define the mechanism of CSAs bactericidal activity, their ability to induce generation of reactive oxygen species (ROS), permeabilization of the inner and outer membranes, and their mechanical and adhesive properties upon CSA addition were examined. Additionally, hemolytic assays were performed to assess CSAs hemocompatibility. Results: All tested CSAs exert substantial bactericidal activity against NDM-1-producing bacteria. Moreover, CSAs significantly prevent biofilm formation as well as reduce the mass of developed biofilms. The mechanism of CSA action comprises both increased permeability of the outer and inner membrane, which is associated with an extensive ROS generation. Additionally, atomic force microscopy (AFM) analysis has shown morphological alterations in bacterial cells and the reduction of stiffness and adhesion properties. Importantly, CSAs are characterized by low hemolytic activity at concentrations that are bactericidal. Conclusion: Development of ceragenins should be viewed as one of the valid strategies to provide new treatment options against infections associated with CPE. The studies presented herein demonstrate that NDM-1-positive bacteria are more susceptible to ceragenins than to conventional antibiotics. In effect, CSA-13, CSA-44, and CSA-131 may be favorable for prevention and decrease of global burden of CPE.

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“…Biofilm formation is an important contributory factor in the establishment and persistence of bacterial infections, and thus is considered a principal reason for antimicrobial resistance [1]. Fossils belonging to different domains of microorganism, archaea and bacteria, and which date back over three billion years have been shown to contain biofilm.…”

Section: Introductionmentioning

confidence: 99%

A Consideration of Antibacterial Agent Efficacies in the Treatment and Prevention of Formation of Staphylococcus aureus Biofilm

Sedarat

Taylor‐Robinson

2019

Journal of Microbiology and Infectious Diseases

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Staphylococcus aureus is a Gram-positive bacterium found frequently on a person's skin and sometimes in their upper respiratory tract. Although regarded primarily as a commensal of the human microbiota S. aureus shows the ability to become an opportunistic pathogen. Hence, it is a common cause of skin and lung infections and of food poisoning. S. aureus forms biofilms, complex communities of bacteria inside an exopolysaccharide matrix, which adhere to different surfaces, including those associated with hospital-acquired infections such as catheters, shunts and other implanted medical devices. In this instance, the presence of proteins adsorbed to the surface of the biomaterial provides a nutrient source for bacterial growth. Due to antimicrobial resistance, use of longstanding antibiotics alone is increasingly an ineffective therapeutic intervention for biofilm-related infections. Therefore, a growing concern is the treatment of medical devices in order to prevent antibiotic resistance associated with routine handling of these items in a healthcare setting. Consequently, several different biotechnological approaches have targeted a practical solution to S. aureus biofilm formation. These include novel antibiotics administered alone or combined with other compounds, application of natural products like enzymes and antimicrobial peptides, and harnessing of nanoparticles and phage therapy. This brief article provides an overview of each of these cutting-edge methods aimed at inhibition of S. aureus biofilms. Development of an effective agent to prevent and treat biofilm formation would represent a significant step forward for infection control of methicillin-resistant S. aureus (MRSA) and other antibiotic-resistant strains that provides a major global public health challenge.

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Effects of ceragenins and conventional antimicrobials on Candida albicans and Staphylococcus aureus mono and multispecies biofilms

Cited by 23 publications

References 38 publications

RETRACTED: Sanguinarine Inhibits Mono- and Dual-Species Biofilm Formation by Candida albicans and Staphylococcus aureus and Induces Mature Hypha Transition of C. albicans

RETRACTED: Sanguinarine Inhibits Mono- and Dual-Species Biofilm Formation by Candida albicans and Staphylococcus aureus and Induces Mature Hypha Transition of C. albicans

<p>NDM-1 Carbapenemase-Producing Enterobacteriaceae are Highly Susceptible to Ceragenins CSA-13, CSA-44, and CSA-131</p>

A Consideration of Antibacterial Agent Efficacies in the Treatment and Prevention of Formation of Staphylococcus aureus Biofilm

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