Effects of Centrifugation on Cell Composition and Viability of Aspirated Adipose Tissue Processed for Transplantation

Condé-Green, Alexandra; Baptista, Leandra Santos; Amorin, Natale Ferreira Gontijo de; Oliveira, Eduardo Duarte de; Silva, Karina Ribeiro da; Pedrosa, Carolina da S. G.; Borojević, Radovan; Pitanguy, Ivo

doi:10.1177/1090820x10369512

Cited by 112 publications

(76 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Adipose tissue harvesting and processing techniques employed in the surgery room play an important role on the viability and integrity of adipose tissue, and according our study, also on the percentage of MSC (Condé-Green et al, 2010). A long-term graft is reached mainly by angiogenesis and MSC enhance local angiogenesis by differentiation events, and secretion of angiogenic factors (Hanson et al, 2010).…”

Section: Fat Graft Enriched With Mesenchymal Stem Cellmentioning

confidence: 53%

“…Our study showed similar histological results. We observed that the adipose tissue resulted from centrifuged lipoaspirate samples contained most of adipocytes with disrupted membranes and general extracellular matrix disruption, whereas decantation maintained the adipocytes´ integrity (Figure 2; Condé-Green et al, 2010). We cannot exclude the fact that reducing centrifugation forces will improve MSC recovery on centrifuged lipoaspirates samples, as already described .…”

Section: Adipose Tissue Harvesting and Preparation For Isolating Mesementioning

confidence: 61%

“…There is no universal agreement on what constitutes an ideal methodology to obtain better graft takes and results. Our results showed that centrifugation, although cleaning adipose tissue of potentially harmful substances yields adipose tissue which is not only devoid of viable adipocytes but also has a diminished percentage of MSC (Condé-Green et al, 2010). Taken together, the long term graft of the implanted centrifuged adipose tissue is less probable, and the implant resorption naturally occurs with clearance of non-viable organic components introduced into the receptor site.…”

Section: Autologous Fat Graftsmentioning

confidence: 71%

“…It is one of the preferred methods of fat processing for soft tissue augmentation. We then investigated how the manipulation of lipoaspirate samples influences in the yield and quality of MSC subsequently isolated in the laboratory (Condé-Green et al, 2010). We have examined lipoaspirates prepared by centrifugation (1228g for 3 minutes) and decantation (30 minutes under the action of gravity).…”

Section: Adipose Tissue Harvesting and Preparation For Isolating Mesementioning

confidence: 99%

“…Possible architectural alterations of adipose tissue caused by these methods was quantified based on the degeneration of adipocytes, staining tissue histological sections with Hematoxilin & Eosin (Condé-Green et al, 2010) or with antibodies against perilipin, an abundant protein in the adipocyte cytoplasmic membrane (Figure 2). Another histological quantification was performed by Rose and colleagues (Rose et al, 2006), showing that decanted samples had twice the quantity of intact adipocytes as compared to centrifuged and washed samples.…”

Section: Adipose Tissue Harvesting and Preparation For Isolating Mesementioning

confidence: 99%

See 4 more Smart Citations

Processing of Lipoaspirate Samples for Optimal Mesenchymal Stem Cells Isolation

Baptista¹,

Silva²,

Pedrosa³

et al. 2011

Advanced Techniques in Liposuction and Fat Transfer

View full text Add to dashboard Cite

Section: Fat Graft Enriched With Mesenchymal Stem Cellmentioning

confidence: 53%

Section: Adipose Tissue Harvesting and Preparation For Isolating Mesementioning

confidence: 61%

Section: Autologous Fat Graftsmentioning

confidence: 71%

Section: Adipose Tissue Harvesting and Preparation For Isolating Mesementioning

confidence: 99%

Section: Adipose Tissue Harvesting and Preparation For Isolating Mesementioning

confidence: 99%

See 3 more Smart Citations

Processing of Lipoaspirate Samples for Optimal Mesenchymal Stem Cells Isolation

Baptista¹,

Silva²,

Pedrosa³

et al. 2011

Advanced Techniques in Liposuction and Fat Transfer

View full text Add to dashboard Cite

Rapid, label‐free enrichment of lymphocytes in a closed system using a flow‐through microfluidic device

Mukhamedshin,

Reddington,

Dinh

et al. 2023

Bioengineering & Transla Med

View full text Add to dashboard Cite

The majority of adoptive cellular therapies are produced from peripheral mononuclear cells obtained via leukapheresis and further enriched for the cells of interest (e.g., T cells). Here, we present a first‐of‐its‐kind closed system, which effectively removes ~85% of monocytes and ~88% of platelets, while recovering ~88% of concentrated T cells in a separate output stream, as the leukapheresis sample flows through a microfluidic device at 5 mL/min. The system is driven by a common peristaltic pump, enabled by a novel pressure wave dampener, and operates in a closed bag‐to‐bag configuration, without requiring any specialized, dedicated equipment. When compared to standard density gradient centrifugation on paired samples, the new system demonstrated a 1.5‐fold increase in T cell recovery and a 2‐fold reduction in inter‐sample variability for this separation outcome. The T cell‐to‐monocyte ratio of the leukapheresis sample was increased to 20:1, whereas with density gradient processing it decreased to 2:1. As a result of superior purity and/or gentler processing, T cells enriched by the system showed a 2.7‐times higher fold expansion during subsequent culture, and an overall 3.5‐times higher cumulative yield. This centrifugation‐free and label‐free closed system for enriching lymphocytes could significantly simplify and standardize the manufacturing of life‐saving cellular therapies.

show abstract

FlexMetric bone marrow aspirator yields laboratory and clinically improved results from mesenchymal stem and progenitor cells without centrifugation

Marx

Amailuk

Patel

et al. 2022

J Tissue Eng Regen Med

View full text Add to dashboard Cite

Several devices used to harvest stem/progenitor cells from bone marrow are available to clinicians. This study compared three devices measuring stem cell yields and correlating those yields to bone regeneration. A flexible forward aspirating system Marrow Marxman (MM), a straight needle aspirating on withdrawal system Marrow Cellutions (MC), and a straight needle aspirating on withdrawal and centrifuging the aspirate (BMAC) were compared in a side‐to‐side patient comparison, as well as tissue engineered bone grafts. The FlexMetric system (MM) produced greater CFU‐f values compared to the straight needle (MC) Δ = 1083/ml, p < 0.001 and 1225/ml, p < 0.001 than the BMAC system. This increased stem/progenitor cell yield also translated into a greater radiographic bone density at 6 months Δ = 88.3 Hu, p ≤ 0.001 versus MC and Δ = 116.7, p < 0.001 versus BMAC at 6 months and Δ = 72.2, p < 0.001 and Δ = 93.3, p < 0.001 at 9 months respectively. The increased stem/progenitor cell yield of the MM system clinically translated into greater bone regeneration as measured by bone volume p < 0.014 and p < 0.001 respectively, trabecular thickness p < 0.007 and p < 0.002 respectively, and trabecular separation p = 0.011 and p < 0.001. A flexible bone marrow aspirator produces higher yields of stem/progenitor cells. Higher yields of stem/progenitor cells translate into greater bone regeneration in tissue engineering. Flexmetric technology produces better bone regeneration due to a forward aspiration concept reducing dilution from peripheral blood and its ability to target lining cells along the inner cortex. Centrifugation systems are not required in tissue engineering procedures involving stem/progenitor cells due to nonviability or functional loss from g‐forces.

show abstract

Effects of Centrifugation on Cell Composition and Viability of Aspirated Adipose Tissue Processed for Transplantation

Abstract: If centrifuged lipoaspirate is used, the pellet (rich in adipose-derived mesenchymal stem cells) and the middle layer should be employed to increase fat graft survival.

Cited by 112 publications

References 21 publications

Processing of Lipoaspirate Samples for Optimal Mesenchymal Stem Cells Isolation

Processing of Lipoaspirate Samples for Optimal Mesenchymal Stem Cells Isolation

Rapid, label‐free enrichment of lymphocytes in a closed system using a flow‐through microfluidic device

FlexMetric bone marrow aspirator yields laboratory and clinically improved results from mesenchymal stem and progenitor cells without centrifugation

Contact Info

Product

Resources

About