Effects of central administration of beta-endorphin on brain and liver DNA synthesis in preweanling rats

Bartolomé, Jorge; Bartolome, Maria B.; Lorber, Brian A.; Dileo, S.J.; Schanberg, Saul M.

doi:10.1016/0306-4522(91)90191-p

Cited by 44 publications

(20 citation statements)

References 29 publications

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“…Opioids have been implicated in the modulation of proliferation of neural cells in vivo and in vitro (Johannesson et al, 1972;Vertes et al, 1982;McLaughlin, 1983, 1991;Kornblum et al, 1987;Bartolome et al, 1991;Stiene-Martin and Hauser, 1991;Barg et al, 1992Barg et al, , 1993a for review, see Hammer and Hauser, 1992). Like many other G protein-coupled receptor systems (Blumer and Johnson, 1994), activation of opioid signal transduction appears to influence cell growth and differentiation by interacting with growth factor-signaling pathways with convergence at the mitogen-activated protein kinase phosphorylation cascade (Avidor-Reiss et al, 1996).…”

mentioning

confidence: 99%

Evidence for κ‐ and μ‐Opioid Receptor Expression in C6 Glioma Cells

Bohn

Belcheva

Coscia

1998

Journal of Neurochemistry

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Abstract:The astrocytoma cell line rat C6 glioma has been used as a model system to study the mechanism of various opioid actions. Nevertheless, the type of opioid receptor(s) involved has not been established. Here we demonstrate the presence of high-affinity U69,593, endomorphin-1, morphine, and /3-endorphin binding in desipramine (DMI)-treated 06 cell membranes by performing homologous and heterologous binding assays with [ 3H}-U69,593, [3H]morphine,or 125l-~3-endorphin.Naive C6 cell membranes displayed U69,593 but neither endomorphin-1, morphine, nor~3-endorphin binding. Cross-linking of 1251-/1-endorphin to C6 membranes gave labeled bands characteristic of opioid receptors. Moreover, RT-PCR analysis of opioid receptor expression in ôontrol and DM1-treated 06 cells indicate that both K-and~t-opioidreceptors are expressed. There does not appear to be a significant difference in the level of~snor K receptor expression in naive versus 06 cells treated with DM1 over a 20-h period. Collectively, the data indicate that K-and ft-opioid receptors are present in 06 glioma cells. Key Words: Opioid receptor-C6 glioma-/3-Endorphin-PCR-U69,593-Morphine-Desipramine.

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mentioning

confidence: 99%

Evidence for κ‐ and μ‐Opioid Receptor Expression in C6 Glioma Cells

Bohn

Belcheva

Coscia

1998

Journal of Neurochemistry

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“…Opioids can function to control growth directly, and through an opioid receptor-mediated interface (e.g., Isayama et al, 1991;Zagon et al, 1996Zagon et al, , 1997, or by way of indirect effects that may or may not be dependent on opioid receptors (e.g., Bartolome et al, 1991). In the case of opioid receptormediated activity, one peptide has emerged as a prominent growth factor: the naturally occurring pentapeptide [Met 5 ]-enkephalin (Murgo, 1985;Isayama et al, 1991;Zagon and McLaughlin, 1993;McLaughlin, 1996;Zagon et al, 1996Zagon et al, , 1997, which is encoded by the proenkephalin A gene.…”

Section: Introductionmentioning

confidence: 99%

“…Endogenous opioid peptides participate in governing the growth of developing, neoplastic, renewing, and healing tissues, and are active in both prokaryotes and eukaryotes McLaughlin, 1983a, b, 1993;Murgo, 1985;Zagon et al, 1986Zagon et al, , 1994Zagon et al, , 1996Zagon et al, , 1997Hauser et al, 1989;Bartolome et al, 1991;Isayama et al, 1991;Meriney et al, 1991;Villiger and Lotz, 1992;Barg et al, 1993;McLaughlin, 1994McLaughlin, , 1996Shahabi and Sharp, 1995;Vertes et al, 1996). Opioids can function to control growth directly, and through an opioid receptor-mediated interface (e.g., Isayama et al, 1991;Zagon et al, 1996Zagon et al, , 1997, or by way of indirect effects that may or may not be dependent on opioid receptors (e.g., Bartolome et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Ontogeny of the opioid growth factor, [Met5]-enkephalin, preproenkephalin gene expression, and the ζ opioid receptor in the developing and adult aorta of rat

McLaughlin

Zagon

1998

Dev. Dyn.

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“…did not alter lung ODC activity, indicating that the effect results indirectly from CNS actions of BE. Centrally mediated effects of CNS BE on other peripheral tissues have been recently reported by our laboratory (14,43,44).…”

Section: Discussionmentioning

confidence: 68%

Effect of Central Administration of β-Endorphin on Lung Ornithine Decarboxylase Activity in Developing Rats

1991

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ABSTRACT. Results from a number of studies suggest a indicates that endogenous opioid systems (i.e. opioids and opioid role for endogenous opioids in the regulation of lung devel-receptors) tonically modulate fetal lung maturation. opment and function. Although it is not known which opioid Accumulated knowledge suggests that BE may influence pulpeptides are involved in these processes, accumulated evi-monary development. Pituitary and hypothalamic levels of BE dence suggests a prominent role for /3-endorphin (BE). Our are elevated in fetal and neonatal rats after maternal morphine study examines the effect of BE on lung ornithine decar-treatment (8). Plasma BE levels in normal newborn babies are boxylase (ODC) activity in preweanling rats. ODC cata-three to four times higher than the concentrations found in their lyzes the rate-limiting step in the synthesis of the poly-mothers, and decline to adult levels by the 5th day of life (9, 10). amines spermidine and spermine, key regulators of cell In infants of heroin-addicted mothers, plasma BE levels show growth, multiplication, and differentiation. Central (but not even larger increases after birth (up to 1000 times adult levels) peripheral) administration of BE reduced lung ODC activ-and remain elevated at 40 d of age (9). These and other obserity by as much as 80% in the 6-d-old rat. Significant vations support the hypothesis that BE may have a prominent decreases in ODC activity were seen at doses of BE as low role in the modulation of lung maturation (1 1-14). as 0.5 pgjg brain wt. In contrast to the reductions in ODC Studies of the consequences of opioid exposure on lung funcactivity, plasma levels of corticosterone in animals admin-tion have predominantly addressed prenatal effects, primarily istered BE were approximately five times higher than those due to their relevance to respiratory distress syndrome. However, seen in control animals. BE'S actions on ODC activity and it is equally important to investigate opioid actions on the plasma corticosterone levels were prevented by naloxone postnatal lung. Both the newborn human and rat lungs are or naltrexone, indicating that both responses are mediated extremely immature and undergo vast morphologic restructuring by opioid receptors. Studies of ODC kinetics showed a after birth; the majority of alveoli are formed postnatally, increasprofound reduction in V,,, (70% below control values), but ing from approximately 20 million at birth to 300 million in the no change in Km. The effect was observed only during the mature lung (15-18). The neonatal lung is highly responsive to first 2 wk of postnatal age, a period of time in lung trophic hormones (19-21). Finally, as the early postnatal lung maturation that is characterized by active alveolarization. development (saccular stage) is fundamentally a continuation of Because changes in ODC levels during early postnatal life the late fetal maturation, the findings obtained during the first are associated with perturbations in tissue growth and/or days of age essentially ...

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Effects of central administration of beta-endorphin on brain and liver DNA synthesis in preweanling rats

Cited by 44 publications

References 29 publications

Evidence for κ‐ and μ‐Opioid Receptor Expression in C6 Glioma Cells

Evidence for κ‐ and μ‐Opioid Receptor Expression in C6 Glioma Cells

Ontogeny of the opioid growth factor, [Met5]-enkephalin, preproenkephalin gene expression, and the ζ opioid receptor in the developing and adult aorta of rat

Effect of Central Administration of β-Endorphin on Lung Ornithine Decarboxylase Activity in Developing Rats

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