Effects of Cellular Senescence on Dental Follicle Cells

Morsczeck, Christian

doi:10.1159/000510014

Cited by 12 publications

(10 citation statements)

References 61 publications

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“…Sox-2, Nanog1 and Nestin, are negative for hematopoietic stem cell markers such as CD11b, CD34, CD45 and HLA-DR [ 46 , 47 ]. Originating from the neural crest origin, hDFPCs can differentiate into osteoblasts, adipocytes, chondrocytes, cementoblasts and periodontal ligament cells as well as neuronal cells [ 47 , 48 ].…”

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

“…hSCAPs are negative for CD14, CD34, CD45, CD150 and HLA-DR expression. These stem cells are also plastic adherent and clonogenic and express low levels of odontoblast markers such as dentin sialoprotein (DSP), matrix extracellular phosphoglycoprotein [ 48 ]. They also have multiple differentiation potentials and can be induced into osteo/odontoblasts, lipoblasts and neuroblasts in vitro [ 49 ].…”

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

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Dental Pulp Stem Cell-Derived Secretome and Its Regenerative Potential

Bär

Lis-Nawara

Grelewski

2021

IJMS

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The therapeutic potential of the dental pulp stem (DSC) cell-derived secretome, consisting of various biomolecules, is undergoing intense research. Despite promising in vitro and in vivo studies, most DSC secretome-based therapies have not been implemented in human medicine because the paracrine effect of the bioactive factors secreted by human dental pulp stem cells (hDPSCs) and human exfoliated deciduous teeth (SHEDs) is not completely understood. In this review, we outline the current data on the hDPSC- and SHED-derived secretome as a potential candidate in the regeneration of bone, cartilage, and nerve tissue. Published reports demonstrate that the dental MSC-derived secretome/conditional medium may be effective in treating neurodegenerative diseases, neural injuries, cartilage defects, and repairing bone by regulating neuroprotective, anti-inflammatory, antiapoptotic, and angiogenic processes through secretome paracrine mechanisms. Dental MSC-secretomes, similarly to the bone marrow MSC-secretome activate molecular and cellular mechanisms, which determine the effectiveness of cell-free therapy. Many reports emphasize that dental MSC-derived secretomes have potential application in tissue-regenerating therapy due to their multidirectional paracrine effect observed in the therapy of many different injured tissues.

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Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

Dental Pulp Stem Cell-Derived Secretome and Its Regenerative Potential

Bär

Lis-Nawara

Grelewski

2021

IJMS

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“…Here, decreased cell proliferation is associated with induction of β-Galactosidase activity and increased cell size, which is typical for senescent cells. Interestingly, while preliminary data showed that dental stem cell markers such as CD105 did not change significantly after induction of cellular senescence, the differentiation potential in senescent DFCs decreased [ 110 , 111 ]. In general, senescent cells control the progression of the cell cycle in the G1 phase by inhibition of cyclin-dependent kinases (CDKs).…”

Section: Molecular Mechanisms Of the Osteogenic Differentiation Of Dfcsmentioning

confidence: 99%

Mechanisms during Osteogenic Differentiation in Human Dental Follicle Cells

Morsczeck

2022

IJMS

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Human dental follicle cells (DFCs) as periodontal progenitor cells are used for studies and research in regenerative medicine and not only in dentistry. Even if innovative regenerative therapies in medicine are often considered the main research area for dental stem cells, these cells are also very useful in basic research and here, for example, for the elucidation of molecular processes in the differentiation into mineralizing cells. This article summarizes the molecular mechanisms driving osteogenic differentiation of DFCs. The positive feedback loop of bone morphogenetic protein (BMP) 2 and homeobox protein DLX3 and a signaling pathway associated with protein kinase B (AKT) and protein kinase C (PKC) are presented and further insights related to other signaling pathways such as the WNT signaling pathway are explained. Subsequently, some works are presented that have investigated epigenetic modifications and non-coding ncRNAs and their connection with the osteogenic differentiation of DFCs. In addition, studies are presented that have shown the influence of extracellular matrix molecules or fundamental biological processes such as cellular senescence on osteogenic differentiation. The putative role of factors associated with inflammatory processes, such as interleukin 8, in osteogenic differentiation is also briefly discussed. This article summarizes the most important insights into the mechanisms of osteogenic differentiation in DFCs and is intended to be a small help in the direction of new research projects in this area.

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“…Therefore, successful bone tissue engineering should involve a combination of abundant MSCs/ osteoprogenitor cells, a suitable mixture of biofactors to induce osteogenic differentiation, and scaffolds based on biomaterials. For dental tissue regeneration, the most eligible are MSCs derived from various dental sources, such as dental pulp-dental pulp stem cells (DPSCs) [13]; periodontal ligament-periodontal ligament stem cells (PDLSCs) [14,15]; gingiva-gingival mesenchymal stem cells (GMSCs) [16,17]; dental follicle or bilayered Hertwig's epithelial root sheath-dental follicle stem cells (DFSCs) [18]; subepithelial palatal soft tissue-palatal-derived stem cells (paldSCs) [19,20]; periapical cyst tissue-human periapical cyst mesenchymal stem cells (hPCy-MSCs) [21,22]; and stem cells from exfoliated deciduous teeth (SHED cells) and from human root apical papilla (SCAP) [23] (Table 1).…”

Section: Osteogenic Potential Of Dental Tissue-derived Mscsmentioning

confidence: 99%

Stem Cells and Their Derivatives—Implications for Alveolar Bone Regeneration: A Comprehensive Review

Hollý

Klein

Mazreku

et al. 2021

IJMS

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Oral and craniofacial bone defects caused by congenital disease or trauma are widespread. In the case of severe alveolar bone defect, autologous bone grafting has been considered a “gold standard”; however, the procedure has several disadvantages, including limited supply, resorption, donor site morbidity, deformity, infection, and bone graft rejection. In the last few decades, bone tissue engineering combined with stem cell-based therapy may represent a possible alternative to current bone augmentation techniques. The number of studies investigating different cell-based bone tissue engineering methods to reconstruct alveolar bone damage is rapidly rising. As an interdisciplinary field, bone tissue engineering combines the use of osteogenic cells (stem cells/progenitor cells), bioactive molecules, and biocompatible scaffolds, whereas stem cells play a pivotal role. Therefore, our work highlights the osteogenic potential of various dental tissue-derived stem cells and induced pluripotent stem cells (iPSCs), the progress in differentiation techniques of iPSCs into osteoprogenitor cells, and the efforts that have been made to fabricate the most suitable and biocompatible scaffold material with osteoinductive properties for successful bone graft generation. Moreover, we discuss the application of stem cell-derived exosomes as a compelling new form of “stem-cell free” therapy.

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Effects of Cellular Senescence on Dental Follicle Cells

Cited by 12 publications

References 61 publications

Dental Pulp Stem Cell-Derived Secretome and Its Regenerative Potential

Dental Pulp Stem Cell-Derived Secretome and Its Regenerative Potential

Mechanisms during Osteogenic Differentiation in Human Dental Follicle Cells

Stem Cells and Their Derivatives—Implications for Alveolar Bone Regeneration: A Comprehensive Review

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