Effects of cellular calcium and magnesium contents on growth of ionophore A23187-treated Lactobacillus lactis.

10 Algal cell biology – important tools to understand metal and herbicide toxicity

“…The antibiotic calcimycin, A23187 , a calcium ionophore has long been used to document calcium signaling in diverse biological systems (Entman et al 1972;Reed and Lardy 1972;Wong et al 1973). While A23187 also transports magnesium , which is another bivalent ion involved in cell signaling (Alatossava 1988;Hand et al 1977;Jeacocke 1993;Marinetti et al 1993;Tissier et al 1993;Zhang et al 1995), its affi nity for calcium is an order of magnitude greater. The calcium channel blocker Verapamil has been used extensively to study calcium channel mediated responses in algae (Alexandrov et al 1990;Ermilova et al 1998;Harz and Hegemann 1991;Love et al 1997;Maier and Calenberg 1994) and Lanthanum , a cation that interferes with calcium binding through competitive interactions with calcium (Jencks and Fujimori 1990;Weiss 1973) have also been used extensively to delineate calcium-mediated processes from other ion-regulated ones (Weiss 1973).…”

Interaction of the calcium ionophore A.23187 (Calcimycin) with Bacillus cereus and Escherichia coli

“…This ionophore has been used to modify the intracellular levels of CaZ+ and Mgz+ both in Gram positive (Alatossava 1988) and Gram negative bacteria. However, in the case of Gram negative rods, it was necessary to make the bacteria permeable to the ionophore by treatment ' COOH 0 * Corresponding author.…”