Effects of cell-penetrating peptides on transduction efficiency of PEGylated adenovirus

Nigatu, Adane S.; Vupputuri, Sravanthi; Flynn, Nicholas; Ramsey, Joshua D.

doi:10.1016/j.biopha.2015.02.015

Cited by 25 publications

(8 citation statements)

References 43 publications

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“…13A). 329 This study provides an example for enhanced TE via virus disassembly, which was suggested in an earlier report covering Pen, Tat and R8 311 and provides the basis for further explanations of observed differences between CPPs. 320 Peptide fibrils.…”

Section: Biomaterials Science Reviewsupporting

confidence: 61%

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Materials promoting viral gene delivery

Kaygisiz

Synatschke

2020

Biomater. Sci.

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Section: Biomaterials Science Reviewsupporting

confidence: 61%

“…11). 311 Coating of CRRR-co-PEG-co-CRRR onto an oncolytic Ad (Fig. 12A) enhanced transduction in tumor sites with a longer blood circulation time and lower liver sequestration was achieved.…”

Section: Review Biomaterials Sciencementioning

confidence: 99%

Materials promoting viral gene delivery

Kaygisiz

Synatschke

2020

Biomater. Sci.

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“…VPs are frequently functionalized with CPPs by the means of a covalent linkage (Figure 2b) via reactive groups of lysine, cysteine, aspartate, or glutamate or via “click chemistry” involving the incorporation of unnatural amino acid residues into a protein sequence [64,65,66,67,68,69,70,71,72,73,74] (Table 4). Although it has been suggested that the noncovalent attachment of CPPs to cargo molecules is preferable for the intracellular delivery of a cargo [27,75], Ad with covalently conjugated Tat has exhibited a 1.5 log higher transduction rate than Ad with a noncovalently associated Tat peptide in the same ratio [65].…”

Section: The Association Of Vps and Cppsmentioning

confidence: 99%

The Utilization of Cell-Penetrating Peptides in the Intracellular Delivery of Viral Nanoparticles

et al. 2019

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Viral particles (VPs) have evolved so as to efficiently enter target cells and to deliver their genetic material. The current state of knowledge allows us to use VPs in the field of biomedicine as nanoparticles that are safe, easy to manipulate, inherently biocompatible, biodegradable, and capable of transporting various cargoes into specific cells. Despite the fact that these virus-based nanoparticles constitute the most common vectors used in clinical practice, the need remains for further improvement in this area. The aim of this review is to discuss the potential for enhancing the efficiency and versatility of VPs via their functionalization with cell-penetrating peptides (CPPs), short peptides that are able to translocate across cellular membranes and to transport various substances with them. The review provides and describes various examples of and means of exploitation of CPPs in order to enhance the delivery of VPs into permissive cells and/or to allow them to enter a broad range of cell types. Moreover, it is possible that CPPs are capable of changing the immunogenic properties of VPs, which could lead to an improvement in their clinical application. The review also discusses strategies aimed at the modification of VPs by CPPs so as to create a useful cargo delivery tool.

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“…Consequently, capsid VLPs have been engineered to take advantage of the unique endosomal environment for triggered drug release in acidic [176,177] and reducing conditions [178,179] typically found within an endosome. In another strategy, fusogenic peptides can be displayed on the capsid VLP surface to insert themselves into the lipid bilayer of the host cell membrane, bypassing endocytosis to achieve direct entry into the cytosol [180,181]. This approach may be useful for sensitive cargos such as siRNA that are not stable in the harsh conditions of the endosomal environment [182].…”

Section: Drug Releasementioning

confidence: 99%

Engineering Virus-like Particles for Antigen and Drug Delivery

Hill

Zak

Khera

et al. 2017

CPPS

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Virus-like particles (VLPs) are nanoscale biological structures consisting of viral proteins assembled in a morphology that mimic the native virion but do not contain the viral genetic material. The possibility of chemically and genetically modifying the proteins contained within VLPs makes them an attractive system for numerous applications. As viruses are potent immune activators as well as natural delivery vehicles of genetic materials to their host cells, VLPs are especially well suited for antigen and drug delivery applications. Despite the great potential, very few VLP designs have made it through clinical trials. In this review, we will discuss the challenges of developing VLPs for antigen and drug delivery, strategies being explored to address these challenges, and the genetic and chemical approaches available for VLP engineering.

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Effects of cell-penetrating peptides on transduction efficiency of PEGylated adenovirus

Cited by 25 publications

References 43 publications

Materials promoting viral gene delivery

Materials promoting viral gene delivery

The Utilization of Cell-Penetrating Peptides in the Intracellular Delivery of Viral Nanoparticles

Engineering Virus-like Particles for Antigen and Drug Delivery

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