Effects of cell-mediated osteoprotegerin gene transfer and mesenchymal stem cell applications on orthodontically induced root resorption of rat teeth

Amuk, Nisa Gül; Kurt, Gökmen; Baran, Yusuf; Seyrantepe, Volkan; Yandım, Melis Kartal; Adan, Aysun; Demir, Secil Akyildiz; Kiraz, Yağmur; Sönmez, Mehmet Fatih

doi:10.1093/ejo/cjw054

Cited by 8 publications

(6 citation statements)

References 27 publications

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“…An orthodontic appliance was applied to all experimental animals and 100 g heavy force was exerted to create OIRR using an orthodontic elastic closed‐coil spring (Grikin Advanced Materials, Beijing, China) fixed between the maxillary left first molar and the incisor (Amuk et al, 2017; Wan Hassan, Stephenson, Waddington, & Sloan, 2012). After 2 weeks, all the coil springs were removed and orthodontic wires were fixed between the maxillary left first molars and the incisors passively to maintain the results of tooth movement (Figure 1a,b).…”

Section: Methodsmentioning

confidence: 99%

Intermittent parathyroid hormone promotes cementogenesis via ephrinB2‐EPHB4 forward signaling

Wang

et al. 2020

Journal Cellular Physiology

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Intermittent parathyroid hormone (PTH) promotes periodontal repair, but the underlying mechanisms remained unclear. Recent studies found that ephrinB2-EPHB4 forward signaling mediated the anabolic effect of PTH in bone homeostasis. Considering the similarities between cementum and bone, we aimed to examine the therapeutic effect of PTH on resorbed roots and explore the role of forward signaling in this process. In vivo experiments showed that intermittent PTH significantly accelerated the regeneration of root resorption and promoted expression of EPHB4 and ephrinB2. When the signaling was blocked, the resorption repair was also delayed. In vitro studies showed that intermittent PTH promoted the expression of EPHB4 and ephrinB2 in OCCM-30 cells. The effects of PTH on the mineralization capacity of OCCM-30 cells was mediated through the ephrinB2-EPHB4 forward signaling. These results support the premise that the anabolic effects of intermittent PTH on the regeneration of root resorption is via the ephrinB2-EPHB4 forward signaling pathway.

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Section: Methodsmentioning

confidence: 99%

Intermittent parathyroid hormone promotes cementogenesis via ephrinB2‐EPHB4 forward signaling

Wang

et al. 2020

Journal Cellular Physiology

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“…The result showed that the cells containing OPG package grew in the animals' PDL and the number of osteoclasts, level of RANKL and bone resorption were reduced significantly after single injection. The level of OPG was highest in the gene therapy group [108].…”

Section: Studies On Genetic Manipulation Of Tooth Movementmentioning

confidence: 88%

“…The gene therapy experiments in orthodontic treatment are still limited to cell cultures or animal experiments [103]. The purposes of previous gene therapy in orthodontic treatment were to investigate the possibility of acceleration of tooth movement or reduction of root resorption by modification of osteoclast differentiation factors such as RANKL or OPG [104][105][106][107][108][109]. The first attempt for gene therapy in orthodontic treatment aimed to transfer OPG gene into periodontal tissue to reduce osteoclast activity and inhibit tooth movement.…”

Section: Studies On Genetic Manipulation Of Tooth Movementmentioning

confidence: 99%

Advances in Orthodontic Tooth Movement: Gene Therapy and Molecular Biology Aspect

Atsawasuwan¹,

Shirazi²

2019

Current Approaches in Orthodontics

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Accelerated orthodontic tooth movement has been recently the topic of interest for orthodontic practitioners. Increased numbers of both clinical and research articles associated with the accelerated orthodontic treatment have been published in peer-reviewed journals in the last couple of years. Biochemical approaches such as administration of drugs, vitamins, and proteins and/or physical approaches such as surgery, vibration, and photobiomodulation have been widely reported and demonstrated the predicted outcome; however, the results are controversial. Very few reports addressed on genetic background of patients or utilization of molecular biological approach on the accelerated orthodontic treatment. In this chapter, we will discuss about biology of tooth movement and how the advances in gene therapy and molecular biology technology would shape the future of orthodontic treatment.

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“…Additionally, BM-MSCs could reduce alveolar bone loss, osteoclast number, inflammatory cell infiltration, and RANKL/OPG ratio in periodontitis-induced bone defect [123,124]. In orthodontic movement, BM-MSC administration, especially BM-MSCs transfected with OPG plasmids, significantly decreased orthodontic force-induced root resorption lacunae, osteoclast number, RANKL, and COX-2 [128].…”

Section: Experimental Therapeutic Applications Of Oral Mscs Inmentioning

confidence: 97%

Oral Mesenchymal Stem/Progenitor Cells: The Immunomodulatory Masters

Zhou

Liu

et al. 2020

Stem Cells International

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Oral mesenchymal stem/progenitor cells (MSCs) are renowned in the field of tissue engineering/regeneration for their multilineage differentiation potential and easy acquisition. These cells encompass the periodontal ligament stem/progenitor cells (PDLSCs), the dental pulp stem/progenitor cells (DPSCs), the stem/progenitor cells from human exfoliated deciduous teeth (SHED), the gingival mesenchymal stem/progenitor cells (GMSCs), the stem/progenitor cells from the apical papilla (SCAP), the dental follicle stem/progenitor cells (DFSCs), the bone marrow mesenchymal stem/progenitor cells (BM-MSCs) from the alveolar bone proper, and the human periapical cyst-mesenchymal stem cells (hPCy-MSCs). Apart from their remarkable regenerative potential, oral MSCs possess the capacity to interact with an inflammatory microenvironment. Although inflammation might affect the properties of oral MSCs, they could inversely exert a multitude of immunological actions to the local inflammatory microenvironment. The present review discusses the current understanding about the immunomodulatory role of oral MSCs both in periodontitis and systemic diseases, their “double-edged sword” uniqueness in inflammatory regulation, their affection of the immune system, and the underlying mechanisms, involving oral MSC-derived extracellular vesicles.

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Effects of cell-mediated osteoprotegerin gene transfer and mesenchymal stem cell applications on orthodontically induced root resorption of rat teeth

Abstract: Taken together all these results, MSCs and GT showed marked inhibition and/or repair effects on OIIRR during orthodontic treatment on rats.

Cited by 8 publications

References 27 publications

Intermittent parathyroid hormone promotes cementogenesis via ephrinB2‐EPHB4 forward signaling

Intermittent parathyroid hormone promotes cementogenesis via ephrinB2‐EPHB4 forward signaling

Advances in Orthodontic Tooth Movement: Gene Therapy and Molecular Biology Aspect

Oral Mesenchymal Stem/Progenitor Cells: The Immunomodulatory Masters

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