Effects of cell concentration, time of fresh storage, and cryopreservation on peripheral blood stem cells

Non‐cryopreserved peripheral blood stem cells as a safe and effective alternative for autologous transplantation in multiple myeloma

“…In our study, all fresh transplanted patients received PBSC after 72 h of cool storage. Our previous laboratorial study substantiated our clinical validation, considering evaluation of graft quality in different storage times and conditions; we have previously validated the best conditions for maintenance of PBSC in refrigerated storage 2 . We emphasize that many factors before, during, and after infusion may interfere in the transplantation outcomes.…”

“…Regarding laboratory implications, we emphasize the importance of monitoring the storage conditions of the PBSC. Besides the time of cool storage, temperature and TNC concentration 2,21 are essential to guarantee the quality maintenance of the product. Factors related to cryopreservation protocols must be considered for cryopreserved grafts 22 .…”

“…In conclusion, the infusion of fresh PBSC stored for up to three days at 4°C (± 2°C) is effective and safe for MM patients under autologous transplantation. We emphasize the importance of TNC/μL quantification up to 24 h, diluted when necessary, considering our previous laboratorial study 2 . We only included grafts maintained cooled with TNC up to 300.000/μL.…”

“…After collection, PBSC was transported to the cellular processing laboratory and stored in the refrigerator at 4°C (± 2°C). Fresh PBSC were maintained in the refrigerator for three days in a concentration up to 300 × 10 3 TNC/μL 2 . For cryopreservation, PBSC were processed until 24 h. PBSC were concentrated by plasma reduction after centrifugation (950 g/10 min/4°C) for a concentration of 600 × 10 3 TNC/μL.…”

“…They are regularly collected by apheresis (peripheral blood stem cells – PBSC), and the product is infused after cryopreservation and storage. However, the fresh maintenance of PBSC is possible while the patient receives the conditioning regimen 2 . Moreover, studies have shown that autologous transplantation with non‐cryopreserved PBSC can be safe and present better outcomes, such as earlier neutrophilic grafting, fewer cases of infusion‐related complications, 3,4 early platelets engraftment, lower incidence of neutropenic fever, less mucositis, fewer days using antibiotic, and less requirement for parenteral nutrition and opioids 3 .…”