Effects of celastrol on human cervical cancer cells as revealed by ion-trap gas chromatography–mass spectrometry based metabolic profiling

Hu, Yingyu; Qi, Yunpeng; Liu, Hua; Fan, Guorong; Chai, Yifeng

doi:10.1016/j.bbagen.2012.10.024

Cited by 25 publications

(17 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Celastrol is a kind natural drug for autoimmune diseases, which can induce apoptosis of various cancer cells, including HeLa cells [ 27 , 28 ]. Therefore, celastrol was chosen as the positive control of apoptosis in this experiment.…”

Section: Resultsmentioning

confidence: 99%

Titanium Dioxide Nanoparticles Induced HeLa Cell Necrosis under UVA Radiation through the ROS-mPTP Pathway

et al. 2020

View full text Add to dashboard Cite

Titanium dioxide nanoparticles (nano-TiO2), as a common nanomaterial, are widely used in water purification, paint, skincare and sunscreens. Its safety has always been a concern. Prior studies have shown that ultraviolet A (UVA) can exacerbate the toxicity of nano-TiO2, including inducing cell apoptosis, changing glycosylation levels, arresting cell cycle, inhibiting tumor cell and bacterial growth. However, whether the combination of UVA and nano-TiO2 cause cell necrosis and its mechanism are still rarely reported. In this study, we investigated the cytotoxicity and phototoxicity of mixture crystalline nano-TiO2 (25% rutile and 75% anatase, 21 nm) under UVA irradiation in HeLa cells. Our results showed that the abnormal membrane integrity and the ultrastructure of HeLa cells, together with the decreased viability induced by nano-TiO2 under UVA irradiation, were due to cell necrosis rather than caspase-dependent apoptosis. Furthermore, nano-TiO2 and UVA generated the reactive oxygen species (ROS) and caused the mitochondrial permeability transition pore (mPTP) of HeLa cells to abnormally open. Cell viability was significantly increased after adding vitamin C (VC) or cyclosporin A (CsA) individually to inhibit ROS and mPTP. Clearance of ROS could not only impede the opening of mPTP but also reduce the rate of cell necrosis. The results suggest the possible mechanism of HeLa cell necrosis caused by nano-TiO2 under UVA irradiation through the ROS-mPTP pathway.

show abstract

Section: Resultsmentioning

confidence: 99%

Titanium Dioxide Nanoparticles Induced HeLa Cell Necrosis under UVA Radiation through the ROS-mPTP Pathway

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In a study of on the effects of celastrol treatment in human cervical cancer cells, Hu et al [28] combined a GC-Ion Trap/MS based metabolomics platform with multivariate statistical analysis and devised a rapid, effective methodology to simultaneously screen multiple metabolic pathways for characteristic anti-cancer drug perturbations.…”

Section: Instrumentation and Toolsmentioning

confidence: 99%

The utility of metabolomics in natural product and biomarker characterization

Cox

Keasling

et al. 2014

Biochimica et Biophysica Acta (BBA) - General Subjects

View full text Add to dashboard Cite

Background Metabolomics is a well-established rapidly developing research field involving quantitative and qualitative metabolite assessment within biological systems. Recent improvements in metabolomics technologies reveal the unequivocal value of metabolomics tools in natural products discovery, gene-function analysis, systems biology and diagnostic platforms. Scope of review We review of some of the prominent metabolomics methodologies employed in data acquisition and analysis of natural products and disease-related biomarkers. Major conclusions This review demonstrates that metabolomics represents a highly adaptable technology with diverse applications ranging from environmental toxicology to disease diagnosis. Metabolomic analysis is shown to provide a unique snapshot of the functional genetic status of an organism by examining its biochemical profile, with relevance toward resolving phylogenetic associations involving horizontal gene transfer and distinguishing subgroups of genera possessing high genetic homology, as well as an increasing role in both elucidating biosynthetic transformations of natural products and detecting preclinical biomarkers of numerous disease states. General significance This review expands the interest in multiplatform combinatorial metabolomic analysis. The applications reviewed range from phylogenetic assignment, biosynthetic transformations of natural products, and the detection of preclinical biomarkers.

show abstract

“…Trypsinization introduces artifacts into metabolomic data (Bi et al 2013 ; Dettmer et al 2011 ; Teng et al 2009 ), imaging is difficult to apply for cells growing in clumps or 3D culture, and common staining agents often display cytotoxic effects (Bielawski et al 2001 ) making downstream applications problematic. Additionally, both methods usually require parallel sampling (Hu et al 2013 ) and are labor-intensive, which is unpractical and inefficient, especially in regard to large studies. To guarantee the immediate quenching of the metabolism, adherent cells are often harvested by scraping the cellular layer in organic extraction solvent (Bi et al 2013 ; Sapcariu et al 2014 ; Dettmer et al 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Removing the bottlenecks of cell culture metabolomics: fast normalization procedure, correlation of metabolites to cell number, and impact of the cell harvesting method

et al. 2016

View full text Add to dashboard Cite

IntroductionAlthough cultured cells are nowadays regularly analyzed by metabolomics technologies, some issues in study setup and data processing are still not resolved to complete satisfaction: a suitable harvesting method for adherent cells, a fast and robust method for data normalization, and the proof that metabolite levels can be normalized to cell number.ObjectivesWe intended to develop a fast method for normalization of cell culture metabolomics samples, to analyze how metabolite levels correlate with cell numbers, and to elucidate the impact of the kind of harvesting on measured metabolite profiles.MethodsWe cultured four different human cell lines and used them to develop a fluorescence-based method for DNA quantification. Further, we assessed the correlation between metabolite levels and cell numbers and focused on the impact of the harvesting method (scraping or trypsinization) on the metabolite profile.ResultsWe developed a fast, sensitive and robust fluorescence-based method for DNA quantification showing excellent linear correlation between fluorescence intensities and cell numbers for all cell lines. Furthermore, 82–97 % of the measured intracellular metabolites displayed linear correlation between metabolite concentrations and cell numbers. We observed differences in amino acids, biogenic amines, and lipid levels between trypsinized and scraped cells.ConclusionWe offer a fast, robust, and validated normalization method for cell culture metabolomics samples and demonstrate the eligibility of the normalization of metabolomics data to the cell number. We show a cell line and metabolite-specific impact of the harvesting method on metabolite concentrations.Electronic supplementary materialThe online version of this article (doi:10.1007/s11306-016-1104-8) contains supplementary material, which is available to authorized users.

show abstract

Effects of celastrol on human cervical cancer cells as revealed by ion-trap gas chromatography–mass spectrometry based metabolic profiling

Cited by 25 publications

References 55 publications

Titanium Dioxide Nanoparticles Induced HeLa Cell Necrosis under UVA Radiation through the ROS-mPTP Pathway

Titanium Dioxide Nanoparticles Induced HeLa Cell Necrosis under UVA Radiation through the ROS-mPTP Pathway

The utility of metabolomics in natural product and biomarker characterization

Removing the bottlenecks of cell culture metabolomics: fast normalization procedure, correlation of metabolites to cell number, and impact of the cell harvesting method

Contact Info

Product

Resources

About