Effects of ceftriaxone induced intestinal dysbacteriosis on lymphocytes in different tissues in mice

Luo, Xia; Zheng, Yue; Wen, Ruyan; Deng, Xiangliang; Zhou, Lian; Liao, Haifeng

doi:10.1016/j.imbio.2016.04.003

Cited by 26 publications

(19 citation statements)

References 18 publications

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“…Splenocytes were prepared by gently pressing the spleen through a sterile 200-gauge steel mesh with the plunger of a syringe. 14 The cytotoxicity of natural killer (NK) cells was assayed by FCM as described previously. 15 Briefly, splenocytes were collected and washed twice with precold PBS at 200× g for 5 minutes at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Fraction From Lycium barbarum Polysaccharides Reduces Immunotoxicity and Enhances Antitumor Activity of Doxorubicin in Mice

Deng

Luo

et al. 2018

Integr Cancer Ther

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The aim of the present study was to investigate whether fraction from Lycium barbarum polysaccharide (LBP) could reduce immunotoxicity and enhance antitumor activity of doxorubicin (Dox) in mice. A water-soluble LBP fraction, designated LBP3, was isolated from edible Chinese herbal Lycium barbarum and used in this study. To investigate the effect of LBP3 on Dox-induced immunotoxicity, tumor-free mice were used and treated with either normal saline, Dox, or Dox plus LBP3. To investigate the effect of LBP3 on antitumor activity of Dox, H22 tumor-bearing mice were used and treated with either normal saline, Dox, LBP3, or Dox plus LBP3. The results showed that LBP3 did not protect against the body weight loss caused by Dox, but it promoted the recovery of body weight starting at day 5 after Dox treatment in tumor-free mice. LBP3 also improved peripheral blood lymphocyte counts, promoted cell cycle recovery in bone marrow cells, and restored the cytotoxicity of natural killer cells. Furthermore, in H22 tumor-bearing mice, LBP3 enhanced antitumor activity of Dox and improved peripheral blood lymphocyte counts and the cytotoxicity of splenocytes. In brief, our results demonstrated that LBP3 could reduce the immunotoxicity and enhance antitumor activity of Dox.

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Section: Methodsmentioning

confidence: 99%

Fraction From Lycium barbarum Polysaccharides Reduces Immunotoxicity and Enhances Antitumor Activity of Doxorubicin in Mice

Deng

Luo

et al. 2018

Integr Cancer Ther

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“…Splenocytes were prepared as described previously with minor modifications [14]. Briefly, splenocytes were isolated from BALB/c mice, and the erythrocytes were lysed with red blood cell lysis buffer (0.829 mg/mL NH 4 Cl, 0.037 mg/mL Na 2 EDTA and 0.100 mg/mL KHCO 3 ).…”

Section: In Vitro Antitumor Assaymentioning

confidence: 99%

Antitumor activity of Lycium barbarum polysaccharides with different molecular weights: an in vitro and in vivo study

Deng

Li²,

Luo

et al. 2017

Food & Nutrition Research

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The antitumor activity of Lycium barbarum polysaccharide (LBP) has been reported, but the structure-bioactivity relationship has still not been fully elucidated. In this study, four watersoluble LBP fractions with serial different molecular weights (MWs) were separated from LBP, designated LBP-2, LBP-3, LBP-4, and LBP-5. After a characteristic analysis, the relationship between MW and antitumor activity of LBP was investigated both in vitro using murine hepatoma H22 cells and in vivo using H22 tumor-bearing mice. In vitro, the results showed that all the LBP fractions had significant inhibition on H22 cells, in which LBP-3 had the best activity. LBP-3 could induce apoptosis, mitochondrial membrane potential destruction, and S phase arrest in H22 cells. In vivo, the results showed that LBP-2, LBP-3, LBP-4, and LBP-5 could inhibit the tumor growth in H22 tumor-bearing mice by 18.18%, 37.97%, 9.09%, and 14.44%, respectively. However, only LBP-3 was able to decrease the tumor weight significantly in H22 tumor-bearing mice. Meanwhile, all the LBP fractions did not show significant toxicity to murine splenocytes, thymus, and spleen. Taken together, these results demonstrated that the antitumor activity of LBP was closely related to its MW, and LBP-3 with medium MW (40-350 kDa) was the main active fraction. ARTICLE HISTORY

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“…Many autoimmune diseases as well as intestinal diseases are related to either declines in the number of lymphocyte cells or to their dysfunction [41][42][43] . In our study, the number of lymphocyte cells increased from the duodenum to ileum in all treatments on all sampling dates.…”

Section: Discussionmentioning

confidence: 99%

Karasinekteki (Musca domestica) Antimikrobiyal Peptidler Salmonella pullorum ile Enfekte Tavuklarda Bağırsak Lactobacillus acidophilus ve Mukozal Epitel Hücrelerini Etkiler

Wang¹,

Wang²,

Zhang³

et al. 2017

Kafkas Univ Vet Fak Derg

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Pullorum disease, which is one the most serious intestinal diseases in poultry production, is generally treated by adding antibiotics to the feed of infected chickens. Although antibiotics are generally quite effective against the disease, they can harm small intestinal flora and mucosa. The objective of this experiment was to determine if antimicrobial peptides (AMPs) from housefly (Musca domestica) larvae can be used to treat pullorum disease. The study included AMPs extracted from Salmonella enteric serovar Pullorum-infected larvae as well as non-infected ones (referred to as induced-AMPs and non-induced AMPs, respectively). Tests were then conducted to determine (i) the activity of these AMPs against S. pullorum and (ii) the effects of the AMPs on intestinal Lactobacillus acidophilus and mucosa epithelial cells in S. pullorum-infected chicks. The results showed that S. pullorum-induced AMPs and non-induced AMPs both exhibited antimicrobial activity against S. pullorum. Small intestinal L. acidophilus populations in convalescent chicks that had been treated with induced AMPs showed similar patterns to those in healthy chicks. Induced AMPs also had relatively little effect on the number of mast cells, lymphocyte cells, and goblet cells in the small intestine of convalescent chicks compared with healthy chicks. In contrast, treatment with antibiotics generally reduced the number of all three cell types, especially in the duodenum. In conclusion, AMPs from housefly larvae offer potential for effective treatment of S. pullorum-infected chickens without the harmful side effects of antibiotics. Keywords: Larvae, Antimicrobial peptide, Salmonella Pullorum, Intestinal, Epithelial cells Karasinekteki (Musca domestica) Antimikrobiyal Peptidler Salmonella pullorum ile Enfekte Tavuklarda Bağırsak Lactobacillus acidophilus ve Mukozal Epitel Hücrelerini Etkiler ÖzetKanatlı üretiminde en ciddi bağırsak hastalıklarından birine neden olan Pullorum hastalığı genellikle enfekte tavukların yemlerine antibiyotik ilavesi ile tedavi edilir. Antibiyotikler genellikle hastalığa karşı oldukça etkili olmakla birlikte ince bağırsak florasına ve mukozaya zarar vermektedir. Bu çalışmanın amacı; karasinek (Musca domestica) larvasındaki antimikrobiyal peptidlerin (AMP) pullorum hastalığının tedavisinde kullanılıp kullanılmayacağının belirlenmesidir. Çalışmada Salmonella enteric serovar Pullorum-enfekte (indüklenmiş AMP) ve enfekte olmayan (indüklenmemiş AMP) larvalardan ekstrakte edilen AMP kullanıldı. Çalışmada; (i) S. pullorum'a karşı AMP aktivitesi ve (ii) S. pullorum-enfekte civcivlerde bağırsak Lactobacillus acidophilus ve mukoza epitel hücrelerinde AMP etkileri araştırıldı. Elde edilen sonuçlar S. pullorum indüklenmiş AMP ve indüklenmemiş AMP'in her ikisinin de S. pullorum'a karşı antimikrobiyal aktivite gösterdiğini ortaya koymuştur. İndüklenmiş AMP uygulanarak tedavi edilen civcivlerin ince bağırsak L. acidophilus popülasyonu sağlıklı civcivlerinki ile benzerlik göstermekteydi. İndüklenmiş AMP; tedavi edilen civcivlerin ince...

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Effects of ceftriaxone induced intestinal dysbacteriosis on lymphocytes in different tissues in mice

Cited by 26 publications

References 18 publications

Fraction From Lycium barbarum Polysaccharides Reduces Immunotoxicity and Enhances Antitumor Activity of Doxorubicin in Mice

Fraction From Lycium barbarum Polysaccharides Reduces Immunotoxicity and Enhances Antitumor Activity of Doxorubicin in Mice

Antitumor activity of Lycium barbarum polysaccharides with different molecular weights: an in vitro and in vivo study

Karasinekteki (Musca domestica) Antimikrobiyal Peptidler Salmonella pullorum ile Enfekte Tavuklarda Bağırsak Lactobacillus acidophilus ve Mukozal Epitel Hücrelerini Etkiler

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