Effects of Cecropin Transgenesis and Interspecific Hybridization on the Resistance to <i>Ichthyophthirius multifiliis</i> in Channel Catfish and Female Channel Catfish × Male Blue Catfish Hybrids

Elaswad, Ahmed; Khalil, Karim; Liu, Yang; Alsaqufi, Ahmed Saud; Abdelrahman, Hisham A.; Su, Baofeng; Perera, Dayan A.; Dong, Sheng; Abass, Nermeen Y.; Dunham, Rex A.

doi:10.1002/naaq.10096

Cited by 10 publications

(11 citation statements)

References 38 publications

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“…The disease resistance against F. columnare and E. ictaluri of F 1 channel catfish possessing cecropin gene was increased by 27.3% and 14.8%, respectively, 162 and cecropintransgenic channel catfish conferred the similar resistance to Ich in hybrid catfish (channel catfish female x blue catfish male). 172 Sarmasik et al 163 applied the same construct into medaka, the result was in agreement with Dunham et al, 162 as the F 2 progeny of cecropintransgenic medaka acquired enhanced resistance to Pseudomonas fluorescens and Vibrio anguillarum infection. In addition, two cecropin constructs, porcine cecropin and one synthetic cecropin analog, were used to generate transgenic rainbow trout, exhibiting resistance against both bacteria (A. salmonicida) and virus (infectious haematopoietic necrosis virus).…”

Section: Transgenessupporting

confidence: 71%

“…The cecropin B gene, first discovered from the moth ( Hyalophora cecropia ), was the first exogenous AMG to be integrated into the genome of fish. The disease resistance against F. columnare and E. ictaluri of F 1 channel catfish possessing cecropin gene was increased by 27.3% and 14.8%, respectively, 162 and cecropin‐transgenic channel catfish conferred the similar resistance to Ich in hybrid catfish (channel catfish female x blue catfish male) 172 . Sarmasik et al 163 applied the same construct into medaka, the result was in agreement with Dunham et al, 162 as the F 2 progeny of cecropin‐transgenic medaka acquired enhanced resistance to Pseudomonas fluorescens and Vibrio anguillarum infection.…”

Section: Application Of Exogenous Amps/amgs In Fishmentioning

confidence: 98%

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Genome‐wide identification of catfish antimicrobial peptides: A new perspective to enhance fish disease resistance

Wang

Dunham

2022

Reviews in Aquaculture

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Antimicrobial peptides (AMPs), also known as host defence peptides, are evolutionarily ancient defensive weapons that can combat infections caused by pathogens. Fish‐derived AMPs have shown microbicidal properties, host immunomodulatory responses, and are induced by a variety of factors, especially pathogenic infection and abiotic stress, which may activate downstream signalling pathways to initiate the expression of antimicrobial peptide genes. Large‐scale genome sequencing has been applied in many aquatic animals, providing databases for the systematic identification of putative AMPs in these species. Recently, the whole genome and the proteome of channel catfish (Ictalurus punctatus) were released; by taking advantage of the antimicrobial peptide database and combining the proteome of catfish, we established a bioinformatic analysis pipeline to identify catfish AMP repertoire. This review introduces an effective method for the identification and development of putative AMPs based on protein datasets, and summarizes the structural properties, immunomodulation and molecular responses in vivo of catfish AMPs. In addition, the evaluation of the antibacterial activity of synthetic or isolated AMPs in vitro, and the applications of AMPs in fish via genetic engineering and clustered regularly interspaced short palindromic repeats associated proteins system (CRISPR/Cas9) were presented, which supports antimicrobial research and aquaculture therapy. The summary and comparison of these AMPs will enhance our understanding and their cross‐species applications. Combined with current genetic engineering and genome editing techniques, it is possible to promote the development of immune processes to protect valuable aquatic animals from infectious diseases.

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Section: Transgenessupporting

confidence: 71%

Section: Application Of Exogenous Amps/amgs In Fishmentioning

confidence: 98%

Genome‐wide identification of catfish antimicrobial peptides: A new perspective to enhance fish disease resistance

Wang

Dunham

2022

Reviews in Aquaculture

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“…plans to expand the population to allow the testing of disease resistance. Cecropin transgenic Channel Catfish exhibited enhanced disease resistance compared to nontransgenic controls (Dunham et al 2002;Elaswad et al 2019). Based on the results of the current study, the cathelicidin transgenic approach should be more effective than the cecropin transgenic approach.…”

Section: Peptidementioning

confidence: 66%

“…2002; Elaswad et al. 2019). Based on the results of the current study, the cathelicidin transgenic approach should be more effective than the cecropin transgenic approach.…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of Cathelicidin Antimicrobial Peptide against Ictalurid Catfish Bacterial Pathogens

et al. 2021

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One of the major goals in aquaculture is to protect fish against infectious diseases as disease outbreaks could lead to economic losses if not controlled. Antimicrobial peptides (AMPs), a class of highly conserved peptides known to possess direct antimicrobial activities against invading pathogens, were evaluated for their ability to protect Channel Catfish Ictalurus punctatus and hybrid catfish (female Channel Catfish × male Blue Catfish I. furcatus) against infection caused by the fish pathogen Aeromonas hydrophila ML09‐119. To identify effective peptides, the minimum inhibitory concentrations against bacterial pathogens Edwardsiella ictaluri S97‐773, Edwardsiella piscicida E22‐10, A. hydrophila ML09‐119, Aeromonas veronii 03X03876, and Flavobacterium columnare GL‐001 were determined in vitro. In general and overall, cathelicidins derived from alligator and sea snake exhibited more potent and rapid antimicrobial activities against the tested catfish pathogens as compared to cecropin and pleurocidin AMPs and ampicillin, the antibiotic control. When the peptides (2.5 µg of peptide/g of fish) were injected into fish and simultaneously challenged with A. hydrophila through immersion, increased survival rates in Channel Catfish and hybrid catfish were observed in both cathelicidin (alligator and sea snake) treatments as compared to other peptides and the infected control (P < 0.001) with alligator cathelicidin being the overall best treatment. Bacterial numbers in the kidney and liver of Channel Catfish and hybrid catfish also decreased (P < 0.05) for cathelicidin‐injected groups at 24 and 48 h after challenge infection. These results show the potential of cathelicidin to protect catfish against bacterial infections and suggest that an approach overexpressing the peptide in transgenic fish, which is the long‐term goal of this research program, may provide a method of decreasing bacterial disease problems in catfish as delivering the peptides via individual injection or feeding would not be economically feasible.

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“…(Zhong et al, 2002;Chiou et al, 2014). However, Elaswad et al (2019) reported that there was a slight increase without significant differences in CSR of cecropintransgenic catfish compared to the wild-type group after the Ichthyophthirius multifiliis infection (55.75% vs 48.70%). Therefore, our meta-findings were supported by these individual studies.…”

Section: High Cumulative Survival Rates Denote Enhanced Resistance To...mentioning

confidence: 99%

Harnessing antimicrobial peptide genes to expedite disease-resistant enhancement in aquaculture: Transgenesis and genome editing

Wang

Cheng

2023

Preprint

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Numerous studies have demonstrated that genome editing and transgenesis by integrating vector-engineered antimicrobial peptide genes (AMGs) is effective to modulate the fish's innate immune system. To generalize the knowledge of AMG application in aquaculture, here we recruited 540 data entries from a pool of empirical studies, which included 18 peer-reviewed publications and spanning 12 diseases. We systematically re-processed and re-analyzed these data by harnessing a cross-disease meta-analysis. On aggregate, AMG-genetic engineering aimed at enhancing disease resistance was shown to decrease the number of colony-forming units of bacteria, improve lysozyme activity, increase the post-infection survival rates, and alter the expression of AMGs and immune-related genes in aquatic animals. Furthermore, the AMG-pathogen combating activity was triggered within two hours after infection and lasted 48 hours, and the overexpression of AMGs was dominant in the spleen and skin, followed by the kidney and liver during this period. Typically, regardless of the type of AMGs, the synergistic expression of AMGs with IL, IKβ, TGFβ, C3b and TLR in AMG-integrated fish contributed to activating inflammatory/immune responses against pathogens. In addition, innovative CRISPR/Cas9-mediated systems enabling the site-directed knock-in of foreign genes at multiple loci were presented and prospected for disease-resistant enhancement in combination with other favorable fish-producing traits, including fast-growing, sterility, and enriched fatty acid. Altogether, our findings indicated that AMGs as transgenes have substantial potential to modulate the fish's innate immune system and accelerate disease-resistant enhancement combined with genetic engineering.

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Effects of Cecropin Transgenesis and Interspecific Hybridization on the Resistance to Ichthyophthirius multifiliis in Channel Catfish and Female Channel Catfish × Male Blue Catfish Hybrids

Cited by 10 publications

References 38 publications

Genome‐wide identification of catfish antimicrobial peptides: A new perspective to enhance fish disease resistance

Genome‐wide identification of catfish antimicrobial peptides: A new perspective to enhance fish disease resistance

Effectiveness of Cathelicidin Antimicrobial Peptide against Ictalurid Catfish Bacterial Pathogens

Harnessing antimicrobial peptide genes to expedite disease-resistant enhancement in aquaculture: Transgenesis and genome editing

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