Effects of Catechin-rich Green Tea Extract on the MMP-1 Activity of HaCaT Keratinocyte Cells and on UVB-induced Skin Damage in Hairless Mice

Abstract

Background: Skin is an organ that protects the human body from various environmental stimuli that can induce immune system activation. Skin aging can be largely divided into two categories: physiological aging, which is caused by the a decreased physiological function of the skin and structural changes with aging, and photoaging, which is caused by the chemical stress induced by external stimuli such as ultraviolet (UV) radiation. Methods and Results: The objective of this study was to investigate the anti-wrinkle and UV protective effect of catechin-rich green tea extract (CGTE) in activated keratinocyte (HaCaT cells) and UV-induced skin damage in hairless mice. The results showed that CGTE inhibits the tumor necrosis factor-alpha interferon-gamma (TNF-α + IFN-γ)-induced expression of matrix metalloproteinase (MMP)-1 in HaCaT cells. In addition, the CGTE treatment significantly reduced wrinkle formation, epidermal thickness, collagen deposition, and transepidermal water loss in dorsal skin irradiated with UVB. However, the β-glucosidase activity was significantly increased. The CGTE treatment inhibits mRNA expression and enzyme activity of MMP-2 and MMP-9 in the dorsal skin irradiated with UVB. Conclusions: It is expected that CGTE can be effectively used as a functional food and cosmetic ingredient to improve skin moisture retention and reduce wrinkle formation.

Protective Effect of Lemon Peel Polyphenols on Oxidative Stress-Induced Damage to Human Keratinocyte HaCaT Cells Through Activation of the Nrf2/HO-1 Signaling Pathway

“…MMP-1 is closely related to human skin aging, and its over-expression negatively affects skin health. Studies have shown that catechins can inhibit MMP-1 activity, thereby protecting the skin (38). (−)-Catechin gallate possesses a good antioxidant effect and promoting cell transport (39).…”

Possible Mechanisms of Oxidative Stress-Induced Skin Cellular Senescence, Inflammation, and Cancer and the Therapeutic Potential of Plant Polyphenols

“…According to Wagn et al, when combined with YAG laser, catechin can reduce wrinkles and pigmentation, increase collagen, and enhance SOD activity in the skin of photodamaged mice [ 233 ]. Catechins isolated from green tea and elm root bark can inhibit MMP-1 expression and may have antiaging effects on fibroblasts, HaCaT cells, and mice [ 164 , 165 , 166 , 167 ]. Green tea catechins were found to prevent skin cellular senescence by regulating NF-κB, AP-1, and MAPK signaling pathways [ 164 ].…”