Effects of Cascading Optical Processes: Part II: Impacts on Experimental Quantification of Sample Absorption and Scattering Properties

Wathudura, Pathum; Wamsley, Max; Wang, Ankai; Chen, Kexun; Nawalage, Samadhi; Wang, Hui; Zou, Shengli; Zhang, Dongmao

doi:10.1021/acs.analchem.2c05055

Cited by 2 publications

(18 citation statements)

References 41 publications

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“…The dPSNP 530 used in this work are simultaneous light absorbers and scatterers with no significant fluorescence activities. 2 The asacquired EOY fluorescence decreases with increasing dPSNP 530 concentration (Figure 4A−F, H). After the absorption IFE on EOY fluorescence is corrected, however, EOY fluorescence increases with increasing dPSNP 530 concentration (Figure 4I).…”

Section: ■ Results and Discussionmentioning

confidence: 96%

“…We demonstrated earlier that the forward scattered light causes a deviation of experimental UV−vis intensity from Beer's law. 1,2 However, eq 3 and eq 4 show that the forward scattered and fluorescence light also introduces spectral distortion due to the fact that the degree of scattering and fluorescence interference can be strongly wavelength dependent. Such interferences are shown with the extinction spectra acquired with both the conventional UV−vis and the recently reported ISUV method for KMnO 4 , EOY, and dPSNP 180 (Figure 1).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…1,3,4 The interplay between light absorption and scattering adds complexity to cascading optical processes in samples that contain both light absorbers and scatterers, making it far more complicated compared to scatterer-only solutions. 2 Light absorption invariably reduces the scattering intensity due to the absorption inner-filter effect (IFE). 5−7 However, the impact of scattering on light absorption is complicated depending on whether the scattered light is taken into consideration.…”

Section: ■ Introductionmentioning

confidence: 99%

“…In such a case, the total light absorption of the scatterer-containing samples is approximately the same as their respective scattererfree counterparts, with the same absorption extinction. 2 The T h i s c o n t e n t i s presence of scatterers in those samples changes the locations where light absorption occurs but does not significantly modify the amount of light absorbed. 2 The present work (Part III) focuses on the cascading optical processes in fluorescent solutions and their impacts on sample UV−vis, scattering, and fluorescence measurements.…”

Section: ■ Introductionmentioning

confidence: 99%

“…2 The T h i s c o n t e n t i s presence of scatterers in those samples changes the locations where light absorption occurs but does not significantly modify the amount of light absorbed. 2 The present work (Part III) focuses on the cascading optical processes in fluorescent solutions and their impacts on sample UV−vis, scattering, and fluorescence measurements. Mechanistically, the cascading optical processes in fluorescent samples can be extraordinarily complicated.…”

Section: ■ Introductionmentioning

confidence: 99%

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Effects of Cascading Optical Processes: Part III. Impacts on Spectroscopic Measurements of Fluorescent Samples

et al. 2023

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Cascading optical processes involve sequential photon–matter interactions triggered by the same individual excitation photons. Parts I and II of this series explored cascading optical processes in scattering-only solutions (Part I) and solutions with light scatterers and absorbers but no emitters (Part II). The current work (Part III) focuses on the effects of cascading optical processes on spectroscopic measurements of fluorescent samples. Four types of samples were examined: (1) eosin Y (EOY), an absorber and emitter; (2) EOY mixed with plain polystyrene nanoparticles (PSNPs), which are pure scatterers; (3) EOY mixed with dyed PSNPs, which scatter and absorb light but do not emit; and (4) fluorescent PSNPs that are simultaneous light absorbers, scatterers, and emitters. Interference from both forward scattered and emitted photons can cause nonlinearity and spectral distortion in UV–vis extinction measurements. Sample absorption by nonfluorogenic chromophores reduces fluorescence intensity, while the effect of scattering on fluorophore fluorescence is complicated by several competing factors. A revised first-principles model is developed for correlating the experimental fluorescence intensity with the sample absorbance in solutions containing both scatterers and absorbers. The optical properties of fluorescent PSNPs of three different sizes were systematically investigated by using integrating-sphere-assisted resonance synchronous spectroscopy, linearly polarized resonance synchronous spectroscopy, UV–vis, and fluorescence spectroscopy. The insights and methodology provided in this work should help improve the reliability of spectroscopic analyses of fluorescent samples, where the interplay among light absorption, scattering, and emission can be complex.

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Section: ■ Results and Discussionmentioning

confidence: 96%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

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Effects of Cascading Optical Processes: Part III. Impacts on Spectroscopic Measurements of Fluorescent Samples

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Advancing Evidence-Based Data Interpretation in UV–Vis and Fluorescence Analysis for Nanomaterials: An Analytical Chemistry Perspective

Wamsley,

Zou,

Zhang

2023

Anal. Chem.

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UV–vis spectrophotometry and spectrofluorometry are indispensable tools in education, research, and industrial process controls with widespread applications in nanoscience encompassing diverse nanomaterials and fields. Nevertheless, the prevailing spectroscopic interpretations and analyses often exhibit ambiguity and errors, particularly evident in the nanoscience literature. This analytical chemistry Perspective focuses on fostering evidence-based data interpretation in experimental studies of materials’ UV–vis absorption, scattering, and fluorescence properties. We begin by outlining common issues observed in UV–vis and fluorescence analysis. Subsequently, we provide a summary of recent advances in commercial UV–vis spectrophotometric and spectrofluorometric instruments, emphasizing their potential to enhance scientific rigor in UV–vis and fluorescence analysis. Furthermore, we propose potential avenues for future developments in spectroscopic instrumentation and measurement strategies, aiming to further augment the utility of optical spectroscopy in nano research for samples where optical complexity surpasses existing tools. Through a targeted focus on the critical issues related to UV–vis and fluorescence properties of nanomaterials, this Perspective can serve as a valuable resource for researchers, educators, and practitioners.

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Effects of Cascading Optical Processes: Part II: Impacts on Experimental Quantification of Sample Absorption and Scattering Properties

Cited by 2 publications

References 41 publications

Effects of Cascading Optical Processes: Part III. Impacts on Spectroscopic Measurements of Fluorescent Samples

Effects of Cascading Optical Processes: Part III. Impacts on Spectroscopic Measurements of Fluorescent Samples

Advancing Evidence-Based Data Interpretation in UV–Vis and Fluorescence Analysis for Nanomaterials: An Analytical Chemistry Perspective

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