Effects of Cartilage Progenitor Cells, Bone Marrow Mesenchymal Stem Cells and Chondrocytes on Cartilage Repair as Seed Cells: An in vitro Study

Gu, Jiaxiang; Wang, Bin; Wang, Tianliang; Zhang, Naichen; Liu, Hongjun; Gui, Jian‐Fang; Lu, Yong

doi:10.2147/dddt.s356936

Cited by 11 publications

(3 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A recent study showed that the chondrogenic differentiation ability of CPCs was higher than that of bone marrow derived-MSCs, indicating that CPCs exhibit a great chondrogenic capacity [ 19 ]. Additionally, a comparative study revealed CPCs showed lower expression of the hypertrophy biomarker Collagen X in cartilage than MSCs [ 20 ]. Furthermore, previous have shown that CPCs could form stratified tissues in cartilage repair and regeneration [ 12 , 21 , 53 , 54 ].…”

Section: Discussionmentioning

confidence: 99%

“…The detection of collagen and GAG content in each group were applied as previously described [ 20 , 41 ]. After CPCs-EVs treatment in cultured mouse cartilage explants for 48 h, the culture medium was removed, washed with PBS twice, and replaced with complete culture medium for 3 days.…”

Section: Methodsmentioning

confidence: 99%

“…Additionally, in vivo studies further confirmed that CPCs combined with platelet-rich plasma (PRP) scaffold showed superiority over bone marrow derived-MSCs combined with PRP scaffold in cartilage regeneration by using a rabbit articular cartilage defect model (critical size, 5 mm) [ 19 ]. Moreover, another study has supported the idea that CPCs have dominance in cell proliferative ability and chondrogenic differentiation capacity as seeding cells on cartilage repair and regeneration, compared with mature chondrocytes and bone marrow derived-MSCs [ 20 ]. Previous comparative research also demonstrated that CPCs are superior to MSCs for application in cartilage regeneration because of their lower expression of collagen X (a biomarker of hypertrophy cartilage) [ 19 – 21 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cartilage progenitor cells derived extracellular vesicles-based cell-free strategy for osteoarthritis treatment by efficient inflammation inhibition and extracellular matrix homeostasis restoration

Feng,

Wang,

Chen

et al. 2024

J Nanobiotechnol

View full text Add to dashboard Cite

Osteoarthritis (OA) is a common degenerative joint disease which currently lacks of effective agents. It is therefore urgent and necessary to seek an effective approach that can inhibit inflammation and promote cartilage matrix homeostasis. Cartilage progenitor cells (CPCs) are identified as a cell population of superficial zone in articular cartilage which possess strong migration ability, proliferative capacity, and chondrogenic potential. Recently, the application of CPCs may represent a novel cell therapy strategy for OA treatment. There is growing evidence that extracellular vesicles (EVs) are primary mediators of the benefits of stem cell-based therapy. In this study, we explored the protective effects of CPCs-derived EVs (CPCs-EVs) on IL-1β-induced chondrocytes. We found CPCs-EVs exhibited chondro-protective effects in vitro. Furthermore, our study demonstrated that CPCs-EVs promoted matrix anabolism and inhibited inflammatory response at least partially via blocking STAT3 activation. In addition, liquid chromatography-tandem mass spectrometry analysis identified 991 proteins encapsulated in CPCs-EVs. By bioinformatics analysis, we showed that STAT3 regulatory proteins were enriched in CPCs-EVs and could be transported to chondrocytes. To promoting the protective function of CPCs-EVs in vivo, CPCs-EVs were modified with cationic peptide ε-polylysine-polyethylene-distearyl phosphatidylethanolamine (PPD) for surface charge reverse. In posttraumatic OA mice, our results showed PPD modified CPCs-EVs (PPD-EVs) effectively inhibited extracellular matrix catabolism and attenuated cartilage degeneration. Moreover, PPD-EVs down-regulated inflammatory factors expressions and reduced OA-related pain in OA mice. In ex-vivo cultured OA cartilage explants, PPD-EVs successfully promoted matrix anabolism and inhibited inflammation. Collectively, CPCs-EVs-based cell-free therapy is a promising strategy for OA treatment.

show abstract