Effects of carrier, sterilisation method, and incubation on survival of Bradyrhizobium japonicum in soybean (Glycine max L.) inoculants

Khavazi, K; Rejali, Farhad; Séguin, Philippe; Miransari, Mohammad

doi:10.1016/j.enzmictec.2007.06.011

Cited by 56 publications

(52 citation statements)

References 14 publications

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“…These findings disagree with those of Khavazi et al (2007); who reported that the number of rhizobia cells in carrier was not significantly different after six months of storage. Lupwayi et al (2000) expressed concern that inoculant quality declines quickly if contaminated.…”

Section: Resultscontrasting

confidence: 57%

Shelf-life of legume inoculants in different carrier materials available in East Africa

Balume¹,

Keya²,

Karanja³

et al. 2015

Afr. Crop Sci. J.

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Adoption of legume inoculation with rhizobia by small-scale farmers in East Africa, and the resultant increase in biological nitrogen fixation requires that quality inoculants meet minimum standards. BIOFIX is one of the commercially available rhizobia/legume inoculants in East Africa, whose standard is at least 10 9 rhizobia g -1. We examined the effect of carrier material and storage conditions on the populations of two industry standard rhizobia, Bradyrhizobium japonicum USDA 110 for soybean (Glycine max) and Rhizobium tropici CIAT 899 for common bean (Phaseolus vulgaris) over 165 days, using the drop plate method on Congo Red Yeast Extract Mannitol Agar. Viable populations of rhizobia differed significantly between carriers and rhizobia strains (P<0.05). Rhizobium tropici CIAT899, prepared with filter mud carrier, achieved a shelf-life of 135 days and B. japonicum USDA110 contained over 10 9 cells g -1 for 105 days. Both of these results fall below the stated six months expiry period of BIOFIX. Replacing filter mud carrier with vermiculite, resulted in an inferior product; although, both more thorough sterilisation and refrigerated storage, after a 14 day curing stage, improved the shelf-life thizobia in the inoculant packet.

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Section: Resultscontrasting

confidence: 57%

Shelf-life of legume inoculants in different carrier materials available in East Africa

Balume¹,

Keya²,

Karanja³

et al. 2015

Afr. Crop Sci. J.

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“…A similar trial on the efficacy of B. cepacia (B3) in the form of bacterial suspension was reported by Sapak et al (2006). The efficacy of using endophytic biological control agent in the field is determined largely by its formulation, shelf life, and delivery techniques (Stephens & Rask 2000;Khavanzi et al 2007;Albareda et al 2008). Successful powder formulation of Burkholderia GanoEB2 using vermiculite as carrier was developed by Nasyaruddin and Idris (2011).…”

Section: Discussionmentioning

confidence: 99%

Effect of formulated bioorganic containing Burkholderia GanoEB2 in suppressing Ganoderma disease in oil palm seedlings

et al. 2015

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“…The maintenance of initial concentrations of Bradyrhizobium cells in inoculant formulations with alternative carrier materials depends on their chemical and physical characteristics (Figueiredo et al, 1992;Khavazi et al, 2007).…”

Section: Resultsmentioning

confidence: 99%

Polymers as carriers for rhizobial inoculant formulations

Fernandes‐Júnior

Rohr

Oliveira

et al. 2009

Pesq. agropec. bras.

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-The aim of this work was to evaluate the effi ciency of carboxymethyl cellulose (CMC) and starch blends as carrier materials of rhizobial inoculants regarding their capacity to maintain viable cells and promote cowpea (Vigna unguiculata) nodulation. The experimental design adopted was completely randomized, with three replicates. Forty different compositions of carboxymethyl cellulose (CMC) with starch, compatibilized or not with different proportions of MgO or ZnO, were evaluated regarding their ability of maintaining rhizobial viable cells during the storage period of one month at room temperature, in an initial screening. Thereafter, selected inoculant carrier blends were evaluated regarding their ability to maintain viable rhizobial cells for a period of 165 days, and their performance as inoculant carriers was compared to a peat-based inoculant carrier under greenhouse conditions. Rhizobial cells were better maintained in blends containing 50-60% CMC. Compatibilizing agents did not increase survival of rhizobial cells for 30 days of storage. The cowpea nodulation of polymer blends was statistically the same of peat-based inoculants. CMC/starch polymer blends are effi cient carriers to rhizobial inoculants for up to 165 days of storage, when compatibilized with MgO (1%).Index terms: Bradyrhizobium japonicum, Vigna unguiculata, biological nitrogen fi xation, carboxymethyl cellulose, inoculant technology, polymer blends. Polímeros como veículos para formulações de inoculantes rizobianosResumo -O objetivo deste trabalho foi avaliar a efi ciência de misturas poliméricas de carboximetilcelulose (CMC) e amido, como veículos de inoculante para rizóbios, quanto à sua capacidade de manter células rizobianas viáveis e promover a nodulação em feijão-caupi (Vigna unguiculata). O delineamento experimental foi completamente casualizado, com três repetições. Quarenta diferentes composições poliméricas de carboximetilcelulose (CMC) e amido, compatibilizadas ou não com proporções de MgO ou ZnO, foram inicialmente avaliadas quanto à sua capacidade de manter células rizobianas viáveis pelo período de um mês. Posteriormente, veículos de inoculantes selecionados foram avaliados quanto à capacidade de manter células rizobianas viáveis pelo período de 165 dias, e seu desempenho como veículos de inoculantes foi comparado com os de inoculantes turfosos, em casa de vegetação. Células rizobianas sobreviveram melhor em misturas com 50-60% de CMC. Os agentes compatibilizantes não aumentaram a sobrevivência das células rizobianas após 30 dias de estocagem. A nodulação do feijão-caupi com o uso das misturas poliméricas não diferiu estatisticamente da nodulação com o uso da turfa. As misturas de CMC/amido, quando compatibilizadas com MgO (1%), são veículos efi cientes para inoculantes rizobianos por até 165 dias de armazenamento.Termos para indexação: Bradyrhizobium japonicum, Vigna unguiculata, fi xação biológica de nitrogênio, carboximetilcelulose, tecnologia de inoculantes, misturas poliméricas.

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Effects of carrier, sterilisation method, and incubation on survival of Bradyrhizobium japonicum in soybean (Glycine max L.) inoculants

Cited by 56 publications

References 14 publications

Shelf-life of legume inoculants in different carrier materials available in East Africa

Shelf-life of legume inoculants in different carrier materials available in East Africa

Effect of formulated bioorganic containing Burkholderia GanoEB2 in suppressing Ganoderma disease in oil palm seedlings

Polymers as carriers for rhizobial inoculant formulations

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