Effects of Carrier and Temperature on Survival of
            <i>Rhizobium</i>
            spp. in Legume Inocula: Development of an Improved Type of Inoculant

Kremer, Robert J.; Peterson, H. L.

doi:10.1128/aem.45.6.1790-1794.1983

Cited by 57 publications

(15 citation statements)

References 11 publications

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“…These results confirm previous works (Mary et al 1985;Mugnier and Jung 1985;Mary et al 1986). The harmful effects of temperature on survival of Rhizobiaceae in the dried state currently described in the literature (Kremer and Peterson 1983;Juwarkar and Rewari 1988;Pesenti-Barili et al 1991) were also observed in this study although satisfactory survival rates were obtained during storage at 3% RH. The conjunction of high RHs ( > 44%) and relatively high temperature resulted in linear decreases in survival rates as previously described (Mary et al 1985;Juwarkar and Rewari 1988).…”

Section: Nodulation and Nitrogen Fixation By Spray-dried Bradyrhizobisupporting

confidence: 75%

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Production and survival during storage of spray‐dried Bradyrhizobium japonicum cell concentrates

Mary

Moschetto

Tailliez

1993

Journal of Applied Bacteriology

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Production of Bradyrhizobium japonicum cell concentrates by spray‐drying in skim milk plus sucrose medium and the feasability of storing dried inocula over long periods were investigated. Storage of spray‐dried cells under mild vacuum was equivalent to storage under nitrogen. Oxygen and ambient temperature were found detrimental for survival of dried cells. High initial cell concentration and storage under low relative humidities (< 23% RH) at 4°C increased the longevity of the inocula (> 109 cfu g‐1 during at least a 25 week storage period) without altering the symbiotic properties of B. japonicum.

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Section: Nodulation and Nitrogen Fixation By Spray-dried Bradyrhizobisupporting

confidence: 75%

“…Drying and protracted storage did not affect the symbiotic properties of B. japonicum. Dye (1982) and Kremer and Peterson (1983) came to similar conclusions although in the report of Dommergues et al (1979) loss of infectiveness has been observed.…”

Section: Nodulation and Nitrogen Fixation By Spray-dried Bradyrhizobisupporting

confidence: 60%

Production and survival during storage of spray‐dried Bradyrhizobium japonicum cell concentrates

Mary

Moschetto

Tailliez

1993

Journal of Applied Bacteriology

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“…In some reports, the rhizobium population fell markedly as most of the water was lost. This rapid decline in survival was followed by a period with a slower death rate (11,17,20,24).…”

Section: Discussionmentioning

confidence: 99%

Rates of Drying and Survival of Rhizobium meliloti Strains During Storage at Different Relative Humidities

Mary

Ochin²,

Tailliez³

1985

Appl Environ Microbiol

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An investigation was made of the survival of six strains of Rhizobium meliloti filtered on membrane filters and held in atmospheres of controlled relative humidities (RH) of from 0 to 100% at 30°C in the presence of air. The rate of water loss in the desiccator was determined by the humidity-controlling solution used. Drying was accelerated by a mild evacuation of the desiccator during the drying step. Survival rates of R. meliloti strains were much higher after slow drying to 0% RH than immediately after rapid drying. Fast drying (drying period less than 3.4 h) was shown to adversely affect the tolerance to storage at all RH values tested (no survival after 2 to 5 days of storage). When survival during storage was measurable (after slow drying), the optimum RH values for storage were 43% for strains A145 and Wu498, 22 to 43% for strains RCR2011, Wu499, and Arl6, and 83% for strain RCR2004. The most favorable drying periods were 8, 9.2, 14.2, and 50.1 h for the subsequent storage of strain RCR2011 at RH values of 0, 22, 43, and 83%, respectively. The damaging effects of rapid drying on the tolerance of strain RCR2011 to storage at different RH values could be prevented either by rehydration and subsequent slow redrying or incomplete rapid drying followed by slow drying. It is suggested that R. meliloti strains are susceptible to desiccation stresses. However, the quantitative differences among strains appear to be large enough to permit selection with regard to tolerance to desiccation and storage in dried states. survival of semidried microorganisms. Can.

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“…This probably accounts for the failure to recover cells grown on MSY, which supported polysaccharide production, and for the considerable improvement in recovery yields when yeast extract alone was the growth medium. Freeze-drying has been used successfully in the long term storage of stock cultures (Dye 1982) and in the production of inoculants (McLeod & Roughley 1961;Kremer & Peterson 1983). It is not widely used for this purpose, however, although it has the advantage of producing a concentrated inocu-lant with a long shelf-life particularly at higher temperatures.…”

Section: Discussionmentioning

confidence: 99%

Production and storage of Rhizobium leguminosarum cell concentrates for use as inoculants

Meade

Higgins

O’Gara

1985

Journal of Applied Bacteriology

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1985. Production and storage of Rhizobium leguminosarum cell concentrates for use as inoculants. Journal of Applied Bacteriology 58, 517-524.Recovery of Rhizobium leguminosurum cells by centrifugation after growth in an industrial fermenter was 100-fold higher when cells were grown on yeast extract (5 g/l) as sole source of carbon and nitrogen when compared with the yields recovered when cells were grown in standard mannitol-yeast extract medium. Methods of storing concentrated suspensions of R. leguminosarum were investigated. Freezedrying caused a marked decrease in viable cell numbers. Viable cell numbers of bacterial concentrates stored in peat decreased steadily from 10"-10'* cfu/g to lo9 cfu/g or less during 26 weeks storage at room temperature or at 4°C. Cell concentrates stored in 40% glycerol at -20°C maintained viable numbers higher than 10" cfu/ml during a 76 week storage period.

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Effects of Carrier and Temperature on Survival of Rhizobium spp. in Legume Inocula: Development of an Improved Type of Inoculant

Cited by 57 publications

References 11 publications

Production and survival during storage of spray‐dried Bradyrhizobium japonicum cell concentrates

Production and survival during storage of spray‐dried Bradyrhizobium japonicum cell concentrates

Rates of Drying and Survival of Rhizobium meliloti Strains During Storage at Different Relative Humidities

Production and storage of Rhizobium leguminosarum cell concentrates for use as inoculants

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