Effects of carboxylesterase gene silence on wheat aphid Sitobion avenae (Fabricius)

Zhang, Yifan; Deng, Fei; Fan, Yongliang; Zhao, Zhangwu

doi:10.1016/j.aspen.2016.03.011

Cited by 3 publications

(4 citation statements)

References 16 publications

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“…[22][23][24] For example, a distal-less gene (Tetur20g03080) was verified as being involved in appendage specification in mites by microinjecting Tetur20g03080-dsRNA and -siRNA. 25 Moreover, RNAi is also beneficial in uncovering the roles of detoxification genes, including CarE, P450 and GST genes, in insects and mites (Sitobion avenae, 26,27 Spodoptera litura, 28 T. cinnabarinus, 29,30 and Helicoverpa armigera 31 ).…”

Section: Introductionmentioning

confidence: 99%

Functional analysis of four upregulated carboxylesterase genes associated with fenpropathrin resistance in Tetranychus cinnabarinus (Boisduval)

Peng

Liu

et al. 2018

Pest Management Science

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Section: Introductionmentioning

confidence: 99%

Functional analysis of four upregulated carboxylesterase genes associated with fenpropathrin resistance in Tetranychus cinnabarinus (Boisduval)

Peng

Liu

et al. 2018

Pest Management Science

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“…NlCarE showed high expression in all stages of Nilaparvata lugens (Lu et al, 2017). The expression level of the Sitobion miscanthi carboxylesterase gradually increased from first to fourth instar larvae and peaked in adults (Zhang et al, 2016). The expression levels of carboxylesterases in different developmental stages are closely related to the functions of the enzymes.…”

Section: Discussionmentioning

confidence: 99%

“…dsRNA has been successfully used for RNAi in a variety of insects, such as Helicoverpa armigera of the Lepidoptera (Gong et al, 2017), Leptinotarsa decemlineata (Yang et al, 2016), and Cylas puncticollis of the Coleoptera (Prentice et al, 2017), Acyrthosiphon pisum (Christiaens et al, 2014), and Adelphocoris suturalis of the Hemiptera (Luo et al, 2017). siRNA has been used for RNAi in H. armigera (Xu et al, 2015), Tribolium castaneum (Wang et al, 2013), Sitobion miscanthi (Zhang et al, 2016), and others. Compared with dsRNA, the synthesis of siRNA is simpler and the cost is lower.…”

Section: Discussionmentioning

confidence: 99%

Decreased carboxylesterase expression associated with increased susceptibility to insecticide in Mythimna separata

Liu

Yang

et al. 2021

Arch Insect Biochem Physiol

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Carboxylesterases are one of the three major types of detoxification enzyme in insects. In this study, we screened 12 full‐length carboxylesterase cDNA sequences from the oriental armyworm Mythimna separata; they were named MsCarE1–MsCarE12 and registered in GenBank with accession numbers MK440541–MK440552. Treatment of fourth instar larvae of M. separata with the LD50 of the insecticide chlorantraniliprole increased the expression levels of MsCarE3 and MsCarE4, while treatment with the LD50 of lambda‐cyhalothrin significantly increased the expression levels of MsCarE5 and MsCarE10. Spatiotemporal expression detection showed that MsCarE3, MsCarE4, MsCarE5, and MsCarE10 were expressed at different developmental stages and in different tissues of M. separata and their expression levels were different. Induction using a high dose of chlorantraniliprole resulted in lower expression of MsCarE3 and MsCarE4. LD50 of lambda‐cyhalothrin induced higher expression of MsCarE5 and MsCarE10, while LD70 induced higher MsCarE10 expression at 3, 6, and 12 h after treatment. RNA interference successfully inhibited the expression of MsCarE3, MsCarE4, MsCarE5, and MsCarE10, to different degrees at different time points. Silencing of MsCarE5, or MsCarE5 and MsCarE10 simultaneously changed carboxylesterase activity and increased the susceptibility of M. separata larvae to lambda‐cyhalothrin. This study provides a new method to increase the insect susceptibility to insecticide.

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“…Two other aphids, the green peach aphid M. persicae and the wheat aphid Sitobion avenae have been extensively studied and efficient oral RNAi was reported in both species. In S. avenae, very low doses of dsRNA, as low as 3-20 ng dsRNA/µL diet were sufficient to elicit successful gene knockdown (Deng and Zhao, 2014;Fan et al, 2015;Zhang et al, 2013;Zhang et al, 2016). In M. persicae, all studies which were retrieved via the systematic literature search used GM plants to elicit RNAi silencing.…”

Section: Hemipteramentioning

confidence: 99%

Literature review of baseline information on RNAi to support the environmental risk assessment of RNAi‐based GM plants

Christiaens

Dzhambazova

Kostov

et al. 2018

EFS3

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This report is the outcome of an EFSA procurement (OC/EFSA/GMO/2015/02) which aims at reviewing relevant scientific information on RNA interference (RNAi) that could serve as baseline information for the environmental risk assessment of RNAi‐based genetically modified (GM) plants. The report is based on a systematic literature search on the use of RNAi molecules in arthropods, nematodes, annelids and molluscs with dsRNA, siRNA and miRNA applied primarily through feeding and soaking (oral ingestion). The numbers of retrieved publications covering these areas are reported, along with the species name, life stages tested, the target gene and its function, details of the test substances and their concentrations used, methods of delivery and effects. Separate sections discuss the available information on: (1) the uptake and systematic spread of RNAi activity, including a description of the various components involved in this process; (2) the mechanisms of dsRNA‐, siRNA‐ and miRNA‐elicited gene silencing and the different factors involved in RNAi efficiency; (3) routes of exposure of the biotic and abiotic environment to dsRNA, siRNA and miRNA from GM plants; (4) the environmental fate of dsRNA, siRNA and miRNA; and (5) the various factors that may limit non‐target effects including exposure, factors influencing the silencing efficiency of dsRNA, siRNA and miRNA, possible unintended and off‐target effects, and their mechanisms. Finally, an overview of the species of arthropods, nematodes, annelids and molluscs for which genomic data are available is also presented. The report identifies some of the challenges involved in developing plants with RNAi systems which affect invertebrate gene expression. The report also concludes that, currently, knowledge on issues such as exposure, specificity, offtarget effects, sequence similarities and bioinformatics is very limited, as only a few RNAi expressing plants which specifically target invertebrate species have been developed and comprehensively studied.

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Effects of carboxylesterase gene silence on wheat aphid Sitobion avenae (Fabricius)

Cited by 3 publications

References 16 publications

Functional analysis of four upregulated carboxylesterase genes associated with fenpropathrin resistance in Tetranychus cinnabarinus (Boisduval)

Functional analysis of four upregulated carboxylesterase genes associated with fenpropathrin resistance in Tetranychus cinnabarinus (Boisduval)

Decreased carboxylesterase expression associated with increased susceptibility to insecticide in Mythimna separata

Literature review of baseline information on RNAi to support the environmental risk assessment of RNAi‐based GM plants

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