Effects of Carbon Sources on Cell Differentiation of Lactobacillus rhamnosus PN04 and Applications

Abstract

Lactobacillus strains are commonly used as probiotics. Formulation for the probiotics is essential. The study has studied the effects of carbon sources including in glucose, fructose, maltose, lactose, saccharose at the different concentrations as 5, 10, 15, 20, 30, 40, 50 g/L on the cell differentiation of Lactobacillus rhamnosus PN04. As results, L. rhamnosus PN04 formed differentiated cells those were called minicells with highly significant number as 7870 minicells in one ml starting culture with the density of cells of 2.57×10 9 cellsin modified MRS broth with lactose 50 g/l.The minicells incubated with paclitaxel (10µg/mL) and cephalosporin (10µg/mL) in 10 hours were detected on antimicrobial activities on Staphylococcus aureus ATCC 25923,Escherichia coli ATCC 9637, Salmonella typhi ATCC 19430, Candida albicans ATCC 14053, Pseudomonas aeruginosa ATCC 27853.By scanning electron microscope, the minicell size was less than 400 nm. The study reported the L. rhamnosus PN04 differentiated cells produced in lactose containing MRS which will play a role in probiotic preparation and drug delivery.