Effects of carbon dioxide on mitochondrial enzymes from <i>Ricinus</i>

Ranson, S. L.; Walker, David A.; Clarke, Ian D.

doi:10.1042/bj0760216

Cited by 46 publications

(23 citation statements)

References 6 publications

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“…Thus, the possibility exists that the slightly expanded pool of CO2 at the slower air flow rate served in the sense of a feedback inhibitor on respiration. However, strong inhibitions of mitochondrial reactions have been observed only at CO2 concentrations which are considerably higher than those which existed in the present study (9,11). More relevant are the studies of Young et al (14) on the effects of 02 and CO2 levels on the climacteric of intact fruits of several species.…”

Section: Aeration Effect On Climacteric and Ripeningmentioning

confidence: 59%

Effects of Air Flow Rate, Storage Temperature, and Harvest Maturity on Respiration and Ripening of Tomato Fruits

Pharr

Kattan

1971

Plant Physiol.

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The interactive effects of aeration rate, storage temperature, harvest maturity, and storage duration on respiration and ripening of tomato fruits (Lycopersicum esculentum var. Roma) were studied. Slow aeration rate strongly reduced the climacteric but did not affect ripening. Low Two replications were conducted. Each sample (15 fruits, about 1 kg) was enclosed in a 3.78-liter jar with gas flow connections. Rates of CO2 evolution were determined daily for 16 consecutive days. In preliminary experiments we observed that both 02 uptake and CO2 evolution were suppressed by slow air flow rates. The suppressed respiration due to slow air flow rate was not accompanied by a shift in the respiratory quotient. Therefore, only CO2 evolution was measured as an index of respiration in the present study.On the 4th, 8th, 12th, and 16th days of storage one sample was removed from each experimental condition for determination of firmness, color, and the concentration of CO2 in the internal atmosphere of the fruits.Respiration. CO, was determined by gas chromatography. A rubber septum was present in the effluent line, and samples were withdrawn into a gas-tight syringe. The 1-ml samples were chromatographed on a column of 60 to 80 mesh silica gel, 24 inches long and one-fourth inch in diameter. Helium was used as the carrier gas at 13 psi, and the column and thermal conductivity detector were maintained at 130 C. CO, production rates were calculated by the formula of Biale (1).Analysis of Internal Gas Atmosphere. Five fruits from each treatment were removed from the respiratory chamber and immediately submerged under water. A hypodermic needle was inserted approximately 1 cm into the core tissue of the stem scar, and a 0.65 ml sample was withdrawn. Samples of 0.5 ml were analyzed under the same chromatographic conditions previously described.Other Analyses. Firmness of five fruits was determined with an Asco firmness meter. A 1 kg prestress load was applied for 10 sec, and a stress load of 1.5 kg was applied for 30 sec (6). All 15 fruits of each sample were frozen intact and later thawed and pulped through a 0.028 mesh screen in a laboratory pulper. Color of the deaerated samples was determined with a Gardner color difference meter standardized with a standard having the values: L, 24.1; a, 24.2; b, 11.6. The data were analyzed by statistical methods of Snedecor (13). RESULTS AND DISCUSSIONTemperature and air flow rate were the strongest variables affecting respiration. Analysis of variance revealed their effects to be independent. All experimental effects were described by two second order interactions: air flow rate X maturity X days of storage and temperature X maturity X days of storage. Certain regression analyses were also conducted.Effect of Air Flow Rate on Respiration and Ripening. The air flow rates which were used in this study were intentionally quite slow. Especially at the slower air flow rate, the system is never in complete equilibrium. We believe that this does not introduce error of sufficient magnitu...

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Section: Aeration Effect On Climacteric and Ripeningmentioning

confidence: 59%

Effects of Air Flow Rate, Storage Temperature, and Harvest Maturity on Respiration and Ripening of Tomato Fruits

Pharr

Kattan

1971

Plant Physiol.

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“…High CO.. concentration in the external atmosplhere was shown to retard the climacteric an'd the ripening of several fruits (12,14,27). At the enzymnatic level it was reported that 10 % CO., inhibite(d markedly succinate oxidation (1,17) and phosphoenolpyruvate carboxylase activity (23). Ranson et al (17) relatedl inhibition of the succinooxidase enzyme to previous observations on succinate accumulation when inhibitory levels of CO., were encountered in fruits.…”

Section: Discussionmentioning

confidence: 99%

Respiration & Internal Atmosphere of Avocado Fruit

1963

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“…Ishikawa et al made a prediction model of gas composition in packaging 7 . The idea of respiratory suppression under high CO 2 condition has been explained by the fact that CO 2 strongly affects mitochondrial activity 4,11,21 . Inaba et al developed an automated system for the simultaneous measurement of respiration rate of horticultural products even in atmospheres containing a considerable amount of CO 2 6 , and Kubo et al reported that the respiratory suppression by high CO 2 was observed on certain crops involving C 2 H 4 production 12,13 .…”

Section: Introductionmentioning

confidence: 99%