Effects of carbon dioxide on the oxidation of succinate and reduced diphosphopyridine nucleotide by <i>Ricinus</i> mitochondria

Bendall, Derek S.; Ranson, S. L.; Walker, David A.

doi:10.1042/bj0760221

Cited by 59 publications

(24 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The alternative oxidase was often fully expressed, probably as a result of the inhibition (30-40%) of the Cyt pathway activity (vcy1) by high levels of carbon dioxide (Table IV). This behavior is consistent with the model of Bahr and Bonner (2) Tables III and IV), and by a quantitatively similar inhibition of the enzymic activity of Cyt c oxidase in callus homogenates (Table V) (3,27,31), and both ATP and PPi dependent phosphofructokinases (9, 10). Miller and Hsu (17) suggested a general sensitivity of mitochondrial dehydrogenases to CO2-bicarbonate mixtures.…”

Section: Discussionsupporting

confidence: 77%

Short-Term Effects of Carbon Dioxide on Carnation Callus Cell Respiration

Palet

Ribas‐Carbó²,

Argilés³

et al. 1991

Plant Physiol.

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 77%

Short-Term Effects of Carbon Dioxide on Carnation Callus Cell Respiration

Palet

Ribas‐Carbó²,

Argilés³

et al. 1991

Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…He observed a strong inhibition of ADP-induced oxygen uptake with succinate but not with other respiratory substrates. Later work of Zeylemaker ( [49]; see also [50]) showed that the bicarbonate ion (HCO;) inhibits isolated succinate dehydrogenase in a competitive manner. Since during glutamate oxidation the oxaloacetate necessary for transamination has to be generated from the oxidation of 2-oxoglutarate via part of the citric-acid cycle (see Fig.…”

Section: P Imentioning

confidence: 99%

Bicarbonate and the Pathway of Glutamate Oxidation in Isolated Rat‐Liver Mitochondria

Wanders¹,

Meijer²,

Groen³

et al. 1983

European Journal of Biochemistry

View full text Add to dashboard Cite

1. The factors affecting the pathway of glutamate oxidation were studied in isolated rat-liver mitochondria in incubations of 2-3 min.2. It was found that bicarbonate at a physiological concentration has a profound effect on the pathway of glutamate oxidation. Ammonia formation via glutamate dehydrogenase is stimulated by bicarbonate [from 5.48 0.73 (n = 8) nmol . min-' . mg protein-'], whereas aspartate formation via the transamination pathway is inhibited [from 38.41 5 2.24 (n = 9) to 24.56 3.28 (n = 6) nmol . min-l . mg protein-' 3.0.29 (n = 10) to 9.57 3. Bicarbonate has no effect on the rate of transport of glutamate via the glutamate-hydroxyl translocator. 4. The interaction of bicarbonate with the pathway of glutamate oxidation occurs primarily at the level of succinate dehydrogenase, due to competitive inhibition of the enzyme by bicarbonate.5. Inhibition by bicarbonate of the transamination pathway leads to a decrease in intramitochondrial 2-oxoglutarate, so that the deamination pathway is stimulated.6. Using an equation which describes flux through glutamate dehydrogenase kinetically, it could be shown that the bicarbonate-induced decrease in intramitochondrial2-oxoglutarate quantitatively accounts for the enhanced rate of deamination.7. It is concluded that in the intact liver flux through glutamate dehydrogenase is sufficient to account for the ammonia formation required for urea synthesis from substrates such as alanine.

show abstract

“…Since CO2 concentration has effects on photosynthesis, respiration, photorespiration, and stomatal behaviour (1,3,(6)(7)(8)10) and inasmuch as it has been suggested that CO2 concentration is involved in the regulation of CAM (2) Gas Sampling. Disposable hypodermic syringes (2.0-ml capacity, 22-gauge needle) were used to withdraw gas samples (0.5-2.0 ml) from photosynthetic tissue.…”

mentioning

confidence: 99%

Relationships between Stomatal Behavior and Internal Carbon Dioxide Concentration in Crassulacean Acid Metabolism Plants

1979

View full text Add to dashboard Cite

Measurements of internal gas phase CO2 concentration, stomatal resistance, and acid content were made in Crassulacean acid metabolism plants growing under The physiological significance of CAM is believed to result from the temporal separation of photosynthetic CO2 reduction and gas exchange with the atmosphere. Gas exchange with the atmosphere is restricted to the hours of darkness when the inevitable associated water loss is likely to be minimized. Photosynthetic CO2 reduction occurs without gas exchange with the atmosphere and hence without water loss. CO2 acquired at night is temporarily stored in malic acid and is consumed in photosynthetic reactions the following day.Earlier work (4, 5) has shown that CO2 may be released to the atmosphere surrounding CAM plants following illumination. This indicates that release of CO2 from malic acid is not directly caused by photosynthetic consumption of CO2 and suggests that there might be an accumulation of CO2 inside illuminated CAM tissues.Since CO2 concentration has effects on photosynthesis, respiration, photorespiration, and stomatal behaviour (1, 3, 6-8, 10) and inasmuch as it has been suggested that CO2 concentration is involved in the regulation of CAM (2) Gas Sampling. Disposable hypodermic syringes (2.0-ml capacity, 22-gauge needle) were used to withdraw gas samples (0.5-2.0 ml) from photosynthetic tissue. The syringe needle was inserted into the tissue through a globule of water standing on the surface of the plant or contained in a cup of Plasticine adhering to the plant. Taking samples by this method ensured, providing that stomata were closed, that any gas entering the syringe came from inside the plant and not from leakage around the needle. Furthermore, as the syringe was withdrawn from the plant this procedure ensured that any partial vacuum in the syringe was filled by water and not by gas from the atmosphere. If the syringe was sealed by plunging the needle deeply into a rubber stopper the sample could be stored for periods of up to 30 min without significant change in CO2 concentration. For the longer periods of storage required for field experiments (up to 30 h) gas samples, usually 1.0 ml, were injected through a serum bottle stopper into small glass vials which contained C02-free N2 and also sand to reduce the internal gas space.Measurement of CO2 Concentration. CO2 was separated from other atmospheric gases by GC of 0.5-ml samples at 70 C on a 2.15-m Porapak Q column using helium at a flow rate of 40 ml/

show abstract

Effects of carbon dioxide on the oxidation of succinate and reduced diphosphopyridine nucleotide by Ricinus mitochondria

Cited by 59 publications

References 7 publications

Short-Term Effects of Carbon Dioxide on Carnation Callus Cell Respiration

Short-Term Effects of Carbon Dioxide on Carnation Callus Cell Respiration

Bicarbonate and the Pathway of Glutamate Oxidation in Isolated Rat‐Liver Mitochondria

Relationships between Stomatal Behavior and Internal Carbon Dioxide Concentration in Crassulacean Acid Metabolism Plants

Contact Info

Product

Resources

About